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Antifibrotic and Regenerative Effects of Treamid in Pulmonary Fibrosis

Idiopathic pulmonary fibrosis (IPF) is a chronic progressive disease characterized by interstitial fibrosis and progressive respiratory failure. Pirfenidone and nintedanib slow down but do not stop the progression of IPF. Thus, new compounds with high antifibrotic activity and simultaneously regener...

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Autores principales: Skurikhin, Evgenii, Nebolsin, Vladimir, Widera, Darius, Ermakova, Natalia, Pershina, Olga, Pakhomova, Angelina, Krupin, Vyacheslav, Pan, Edgar, Zhukova, Mariia, Novikov, Fedor, Sandrikina, Lubov, Morozov, Sergey, Kubatiev, Aslan, Dygai, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664690/
https://www.ncbi.nlm.nih.gov/pubmed/33171668
http://dx.doi.org/10.3390/ijms21218380
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author Skurikhin, Evgenii
Nebolsin, Vladimir
Widera, Darius
Ermakova, Natalia
Pershina, Olga
Pakhomova, Angelina
Krupin, Vyacheslav
Pan, Edgar
Zhukova, Mariia
Novikov, Fedor
Sandrikina, Lubov
Morozov, Sergey
Kubatiev, Aslan
Dygai, Alexander
author_facet Skurikhin, Evgenii
Nebolsin, Vladimir
Widera, Darius
Ermakova, Natalia
Pershina, Olga
Pakhomova, Angelina
Krupin, Vyacheslav
Pan, Edgar
Zhukova, Mariia
Novikov, Fedor
Sandrikina, Lubov
Morozov, Sergey
Kubatiev, Aslan
Dygai, Alexander
author_sort Skurikhin, Evgenii
collection PubMed
description Idiopathic pulmonary fibrosis (IPF) is a chronic progressive disease characterized by interstitial fibrosis and progressive respiratory failure. Pirfenidone and nintedanib slow down but do not stop the progression of IPF. Thus, new compounds with high antifibrotic activity and simultaneously regenerative activity are an unmet clinical need. Recently, we showed that Treamid can help restoring the pancreas and testicular tissue in mice with metabolic disorders. We hypothesized that Treamid may be effective in antifibrotic therapy and regeneration of damaged lung tissue in pulmonary fibrosis. In this study, experiments were performed on male C57BL/6 mice with bleomycin-induced pulmonary fibrosis. We applied histological and immunohistochemical methods, ELISA, and assessed the expression of markers of endothelial and epithelial cells in primary cultures of CD31(+) and CD326(+) lung cells. Finally, we evaluated esterase activity and apoptosis of lung cells in vitro. Our data indicate that Treamid exhibits antifibrotic activity in mice with pulmonary fibrosis and has a positive effect on capillaries of the lungs. Treamid also increases the number of endothelial progenitor cells in the lungs of animals with pulmonary fibrosis. Lastly, Treamid increases esterase activity and decreases apoptosis of CD31(+) lung cells in vitro. Based on these findings, we suggest that Treamid may represent a promising compound for the development of new antifibrotic agents, which are capable of stimulating regeneration of lung endothelium in IPF patients.
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spelling pubmed-76646902020-11-14 Antifibrotic and Regenerative Effects of Treamid in Pulmonary Fibrosis Skurikhin, Evgenii Nebolsin, Vladimir Widera, Darius Ermakova, Natalia Pershina, Olga Pakhomova, Angelina Krupin, Vyacheslav Pan, Edgar Zhukova, Mariia Novikov, Fedor Sandrikina, Lubov Morozov, Sergey Kubatiev, Aslan Dygai, Alexander Int J Mol Sci Article Idiopathic pulmonary fibrosis (IPF) is a chronic progressive disease characterized by interstitial fibrosis and progressive respiratory failure. Pirfenidone and nintedanib slow down but do not stop the progression of IPF. Thus, new compounds with high antifibrotic activity and simultaneously regenerative activity are an unmet clinical need. Recently, we showed that Treamid can help restoring the pancreas and testicular tissue in mice with metabolic disorders. We hypothesized that Treamid may be effective in antifibrotic therapy and regeneration of damaged lung tissue in pulmonary fibrosis. In this study, experiments were performed on male C57BL/6 mice with bleomycin-induced pulmonary fibrosis. We applied histological and immunohistochemical methods, ELISA, and assessed the expression of markers of endothelial and epithelial cells in primary cultures of CD31(+) and CD326(+) lung cells. Finally, we evaluated esterase activity and apoptosis of lung cells in vitro. Our data indicate that Treamid exhibits antifibrotic activity in mice with pulmonary fibrosis and has a positive effect on capillaries of the lungs. Treamid also increases the number of endothelial progenitor cells in the lungs of animals with pulmonary fibrosis. Lastly, Treamid increases esterase activity and decreases apoptosis of CD31(+) lung cells in vitro. Based on these findings, we suggest that Treamid may represent a promising compound for the development of new antifibrotic agents, which are capable of stimulating regeneration of lung endothelium in IPF patients. MDPI 2020-11-08 /pmc/articles/PMC7664690/ /pubmed/33171668 http://dx.doi.org/10.3390/ijms21218380 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Skurikhin, Evgenii
Nebolsin, Vladimir
Widera, Darius
Ermakova, Natalia
Pershina, Olga
Pakhomova, Angelina
Krupin, Vyacheslav
Pan, Edgar
Zhukova, Mariia
Novikov, Fedor
Sandrikina, Lubov
Morozov, Sergey
Kubatiev, Aslan
Dygai, Alexander
Antifibrotic and Regenerative Effects of Treamid in Pulmonary Fibrosis
title Antifibrotic and Regenerative Effects of Treamid in Pulmonary Fibrosis
title_full Antifibrotic and Regenerative Effects of Treamid in Pulmonary Fibrosis
title_fullStr Antifibrotic and Regenerative Effects of Treamid in Pulmonary Fibrosis
title_full_unstemmed Antifibrotic and Regenerative Effects of Treamid in Pulmonary Fibrosis
title_short Antifibrotic and Regenerative Effects of Treamid in Pulmonary Fibrosis
title_sort antifibrotic and regenerative effects of treamid in pulmonary fibrosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664690/
https://www.ncbi.nlm.nih.gov/pubmed/33171668
http://dx.doi.org/10.3390/ijms21218380
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