Cargando…
Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
Cancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent eviden...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664935/ https://www.ncbi.nlm.nih.gov/pubmed/33182448 http://dx.doi.org/10.3390/molecules25215216 |
_version_ | 1783609923453583360 |
---|---|
author | Choi, Jung-Won Lim, Soyeon Kang, Jung Hwa Hwang, Sung Hwan Hwang, Ki-Chul Kim, Sang Woo Lee, Seahyoung |
author_facet | Choi, Jung-Won Lim, Soyeon Kang, Jung Hwa Hwang, Sung Hwan Hwang, Ki-Chul Kim, Sang Woo Lee, Seahyoung |
author_sort | Choi, Jung-Won |
collection | PubMed |
description | Cancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent evidence indicates that even immune-cell-released vesicles such as NK-cell-derived exosomes also exert anticancer effect. Nevertheless, the underlying mechanisms remain elusive. In the present study, the anticancer potential of isolated extracellular vesicles (EVs) from expanded and activated NK-cell-enriched lymphocytes (NKLs) prepared by house-developed protocol was evaluated both in vitro and in vivo. Moreover, isolated EVs were characterized by using two-dimensional electrophoresis (2-DE)-based proteome and network analysis, and functional study using identified factors was performed. Our data indicated that the EVs from expanded and active NKLs had anticancer properties, and a number of molecules, such as Fas ligand, TRAIL, NKG2D, β-actin, and fibrinogen, were identified as effector candidates based on the proteome analysis and functional study. The results of the present study suggest the possibility of NK-cell-derived EVs as a viable immunotherapeutic strategy for cancer. |
format | Online Article Text |
id | pubmed-7664935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76649352020-11-14 Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors Choi, Jung-Won Lim, Soyeon Kang, Jung Hwa Hwang, Sung Hwan Hwang, Ki-Chul Kim, Sang Woo Lee, Seahyoung Molecules Article Cancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent evidence indicates that even immune-cell-released vesicles such as NK-cell-derived exosomes also exert anticancer effect. Nevertheless, the underlying mechanisms remain elusive. In the present study, the anticancer potential of isolated extracellular vesicles (EVs) from expanded and activated NK-cell-enriched lymphocytes (NKLs) prepared by house-developed protocol was evaluated both in vitro and in vivo. Moreover, isolated EVs were characterized by using two-dimensional electrophoresis (2-DE)-based proteome and network analysis, and functional study using identified factors was performed. Our data indicated that the EVs from expanded and active NKLs had anticancer properties, and a number of molecules, such as Fas ligand, TRAIL, NKG2D, β-actin, and fibrinogen, were identified as effector candidates based on the proteome analysis and functional study. The results of the present study suggest the possibility of NK-cell-derived EVs as a viable immunotherapeutic strategy for cancer. MDPI 2020-11-09 /pmc/articles/PMC7664935/ /pubmed/33182448 http://dx.doi.org/10.3390/molecules25215216 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Choi, Jung-Won Lim, Soyeon Kang, Jung Hwa Hwang, Sung Hwan Hwang, Ki-Chul Kim, Sang Woo Lee, Seahyoung Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
title | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
title_full | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
title_fullStr | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
title_full_unstemmed | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
title_short | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
title_sort | proteome analysis of human natural killer cell derived extracellular vesicles for identification of anticancer effectors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664935/ https://www.ncbi.nlm.nih.gov/pubmed/33182448 http://dx.doi.org/10.3390/molecules25215216 |
work_keys_str_mv | AT choijungwon proteomeanalysisofhumannaturalkillercellderivedextracellularvesiclesforidentificationofanticancereffectors AT limsoyeon proteomeanalysisofhumannaturalkillercellderivedextracellularvesiclesforidentificationofanticancereffectors AT kangjunghwa proteomeanalysisofhumannaturalkillercellderivedextracellularvesiclesforidentificationofanticancereffectors AT hwangsunghwan proteomeanalysisofhumannaturalkillercellderivedextracellularvesiclesforidentificationofanticancereffectors AT hwangkichul proteomeanalysisofhumannaturalkillercellderivedextracellularvesiclesforidentificationofanticancereffectors AT kimsangwoo proteomeanalysisofhumannaturalkillercellderivedextracellularvesiclesforidentificationofanticancereffectors AT leeseahyoung proteomeanalysisofhumannaturalkillercellderivedextracellularvesiclesforidentificationofanticancereffectors |