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Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors

Cancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent eviden...

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Autores principales: Choi, Jung-Won, Lim, Soyeon, Kang, Jung Hwa, Hwang, Sung Hwan, Hwang, Ki-Chul, Kim, Sang Woo, Lee, Seahyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664935/
https://www.ncbi.nlm.nih.gov/pubmed/33182448
http://dx.doi.org/10.3390/molecules25215216
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author Choi, Jung-Won
Lim, Soyeon
Kang, Jung Hwa
Hwang, Sung Hwan
Hwang, Ki-Chul
Kim, Sang Woo
Lee, Seahyoung
author_facet Choi, Jung-Won
Lim, Soyeon
Kang, Jung Hwa
Hwang, Sung Hwan
Hwang, Ki-Chul
Kim, Sang Woo
Lee, Seahyoung
author_sort Choi, Jung-Won
collection PubMed
description Cancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent evidence indicates that even immune-cell-released vesicles such as NK-cell-derived exosomes also exert anticancer effect. Nevertheless, the underlying mechanisms remain elusive. In the present study, the anticancer potential of isolated extracellular vesicles (EVs) from expanded and activated NK-cell-enriched lymphocytes (NKLs) prepared by house-developed protocol was evaluated both in vitro and in vivo. Moreover, isolated EVs were characterized by using two-dimensional electrophoresis (2-DE)-based proteome and network analysis, and functional study using identified factors was performed. Our data indicated that the EVs from expanded and active NKLs had anticancer properties, and a number of molecules, such as Fas ligand, TRAIL, NKG2D, β-actin, and fibrinogen, were identified as effector candidates based on the proteome analysis and functional study. The results of the present study suggest the possibility of NK-cell-derived EVs as a viable immunotherapeutic strategy for cancer.
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spelling pubmed-76649352020-11-14 Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors Choi, Jung-Won Lim, Soyeon Kang, Jung Hwa Hwang, Sung Hwan Hwang, Ki-Chul Kim, Sang Woo Lee, Seahyoung Molecules Article Cancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent evidence indicates that even immune-cell-released vesicles such as NK-cell-derived exosomes also exert anticancer effect. Nevertheless, the underlying mechanisms remain elusive. In the present study, the anticancer potential of isolated extracellular vesicles (EVs) from expanded and activated NK-cell-enriched lymphocytes (NKLs) prepared by house-developed protocol was evaluated both in vitro and in vivo. Moreover, isolated EVs were characterized by using two-dimensional electrophoresis (2-DE)-based proteome and network analysis, and functional study using identified factors was performed. Our data indicated that the EVs from expanded and active NKLs had anticancer properties, and a number of molecules, such as Fas ligand, TRAIL, NKG2D, β-actin, and fibrinogen, were identified as effector candidates based on the proteome analysis and functional study. The results of the present study suggest the possibility of NK-cell-derived EVs as a viable immunotherapeutic strategy for cancer. MDPI 2020-11-09 /pmc/articles/PMC7664935/ /pubmed/33182448 http://dx.doi.org/10.3390/molecules25215216 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Choi, Jung-Won
Lim, Soyeon
Kang, Jung Hwa
Hwang, Sung Hwan
Hwang, Ki-Chul
Kim, Sang Woo
Lee, Seahyoung
Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
title Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
title_full Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
title_fullStr Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
title_full_unstemmed Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
title_short Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
title_sort proteome analysis of human natural killer cell derived extracellular vesicles for identification of anticancer effectors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664935/
https://www.ncbi.nlm.nih.gov/pubmed/33182448
http://dx.doi.org/10.3390/molecules25215216
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