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Longitudinal Serological Analysis and Neutralizing Antibody Levels in Coronavirus Disease 2019 Convalescent Patients
BACKGROUND: Understanding the longitudinal trajectory of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is crucial for diagnosis of prior infection and predicting future immunity. METHODS: We conducted a longitudinal analysis of coronavirus disease 2019 convalescent patients...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665595/ https://www.ncbi.nlm.nih.gov/pubmed/33140086 http://dx.doi.org/10.1093/infdis/jiaa659 |
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author | Muecksch, Frauke Wise, Helen Batchelor, Becky Squires, Maria Semple, Elizabeth Richardson, Claire McGuire, Jacqueline Clearly, Sarah Furrie, Elizabeth Greig, Neil Hay, Gordon Templeton, Kate Lorenzi, Julio C C Hatziioannou, Theodora Jenks, Sara Bieniasz, Paul D |
author_facet | Muecksch, Frauke Wise, Helen Batchelor, Becky Squires, Maria Semple, Elizabeth Richardson, Claire McGuire, Jacqueline Clearly, Sarah Furrie, Elizabeth Greig, Neil Hay, Gordon Templeton, Kate Lorenzi, Julio C C Hatziioannou, Theodora Jenks, Sara Bieniasz, Paul D |
author_sort | Muecksch, Frauke |
collection | PubMed |
description | BACKGROUND: Understanding the longitudinal trajectory of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is crucial for diagnosis of prior infection and predicting future immunity. METHODS: We conducted a longitudinal analysis of coronavirus disease 2019 convalescent patients, with neutralizing antibody assays and SARS-CoV-2 serological assay platforms using SARS-CoV-2 spike (S) or nucleocapsid (N) antigens. RESULTS: Sensitivities of serological assays in diagnosing prior SARS-CoV-2 infection changed with time. One widely used commercial platform that had an initial sensitivity of >95% declined to 71% at 81–100 days after diagnosis. The trajectories of median binding antibody titers measured over approximately 3–4 months were not dependent on the use of SARS-CoV-2 N or S proteins as antigen. The median neutralization titer decreased by approximately 45% per month. Each serological assay gave quantitative antibody titers that were correlated with SARS-CoV-2 neutralization titers, but S-based serological assay measurements better predicted neutralization potency. Correlation between S-binding and neutralization titers deteriorated with time, and decreases in neutralization titers were not predicted by changes in S-binding antibody titers. CONCLUSIONS: Different SARS-CoV-2 serological assays are more or less well suited for surveillance versus prediction of serum neutralization potency. Extended follow-up should facilitate the establishment of appropriate serological correlates of protection against SARS-CoV-2 reinfection. |
format | Online Article Text |
id | pubmed-7665595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-76655952020-11-16 Longitudinal Serological Analysis and Neutralizing Antibody Levels in Coronavirus Disease 2019 Convalescent Patients Muecksch, Frauke Wise, Helen Batchelor, Becky Squires, Maria Semple, Elizabeth Richardson, Claire McGuire, Jacqueline Clearly, Sarah Furrie, Elizabeth Greig, Neil Hay, Gordon Templeton, Kate Lorenzi, Julio C C Hatziioannou, Theodora Jenks, Sara Bieniasz, Paul D J Infect Dis Major Articles and Brief Reports BACKGROUND: Understanding the longitudinal trajectory of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is crucial for diagnosis of prior infection and predicting future immunity. METHODS: We conducted a longitudinal analysis of coronavirus disease 2019 convalescent patients, with neutralizing antibody assays and SARS-CoV-2 serological assay platforms using SARS-CoV-2 spike (S) or nucleocapsid (N) antigens. RESULTS: Sensitivities of serological assays in diagnosing prior SARS-CoV-2 infection changed with time. One widely used commercial platform that had an initial sensitivity of >95% declined to 71% at 81–100 days after diagnosis. The trajectories of median binding antibody titers measured over approximately 3–4 months were not dependent on the use of SARS-CoV-2 N or S proteins as antigen. The median neutralization titer decreased by approximately 45% per month. Each serological assay gave quantitative antibody titers that were correlated with SARS-CoV-2 neutralization titers, but S-based serological assay measurements better predicted neutralization potency. Correlation between S-binding and neutralization titers deteriorated with time, and decreases in neutralization titers were not predicted by changes in S-binding antibody titers. CONCLUSIONS: Different SARS-CoV-2 serological assays are more or less well suited for surveillance versus prediction of serum neutralization potency. Extended follow-up should facilitate the establishment of appropriate serological correlates of protection against SARS-CoV-2 reinfection. Oxford University Press 2020-11-03 /pmc/articles/PMC7665595/ /pubmed/33140086 http://dx.doi.org/10.1093/infdis/jiaa659 Text en © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Major Articles and Brief Reports Muecksch, Frauke Wise, Helen Batchelor, Becky Squires, Maria Semple, Elizabeth Richardson, Claire McGuire, Jacqueline Clearly, Sarah Furrie, Elizabeth Greig, Neil Hay, Gordon Templeton, Kate Lorenzi, Julio C C Hatziioannou, Theodora Jenks, Sara Bieniasz, Paul D Longitudinal Serological Analysis and Neutralizing Antibody Levels in Coronavirus Disease 2019 Convalescent Patients |
title | Longitudinal Serological Analysis and Neutralizing Antibody Levels in Coronavirus Disease 2019 Convalescent Patients |
title_full | Longitudinal Serological Analysis and Neutralizing Antibody Levels in Coronavirus Disease 2019 Convalescent Patients |
title_fullStr | Longitudinal Serological Analysis and Neutralizing Antibody Levels in Coronavirus Disease 2019 Convalescent Patients |
title_full_unstemmed | Longitudinal Serological Analysis and Neutralizing Antibody Levels in Coronavirus Disease 2019 Convalescent Patients |
title_short | Longitudinal Serological Analysis and Neutralizing Antibody Levels in Coronavirus Disease 2019 Convalescent Patients |
title_sort | longitudinal serological analysis and neutralizing antibody levels in coronavirus disease 2019 convalescent patients |
topic | Major Articles and Brief Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665595/ https://www.ncbi.nlm.nih.gov/pubmed/33140086 http://dx.doi.org/10.1093/infdis/jiaa659 |
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