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Transcription termination and antitermination of bacterial CRISPR arrays
A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repeated sequences (‘repeats’) interspersed with short, variable sequences (‘spacers’). CRISPR arrays are transcribed and processed into individual CRISPR RNAs that each include a single spacer, and d...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665894/ https://www.ncbi.nlm.nih.gov/pubmed/33124980 http://dx.doi.org/10.7554/eLife.58182 |
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author | Stringer, Anne M Baniulyte, Gabriele Lasek-Nesselquist, Erica Seed, Kimberley D Wade, Joseph T |
author_facet | Stringer, Anne M Baniulyte, Gabriele Lasek-Nesselquist, Erica Seed, Kimberley D Wade, Joseph T |
author_sort | Stringer, Anne M |
collection | PubMed |
description | A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repeated sequences (‘repeats’) interspersed with short, variable sequences (‘spacers’). CRISPR arrays are transcribed and processed into individual CRISPR RNAs that each include a single spacer, and direct Cas proteins to complementary sequences in invading nucleic acid. Most bacterial CRISPR array transcripts are unusually long for untranslated RNA, suggesting the existence of mechanisms to prevent premature transcription termination by Rho, a conserved bacterial transcription termination factor that rapidly terminates untranslated RNA. We show that Rho can prematurely terminate transcription of bacterial CRISPR arrays, and we identify a widespread antitermination mechanism that antagonizes Rho to facilitate complete transcription of CRISPR arrays. Thus, our data highlight the importance of transcription termination and antitermination in the evolution of bacterial CRISPR-Cas systems. |
format | Online Article Text |
id | pubmed-7665894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-76658942020-11-16 Transcription termination and antitermination of bacterial CRISPR arrays Stringer, Anne M Baniulyte, Gabriele Lasek-Nesselquist, Erica Seed, Kimberley D Wade, Joseph T eLife Genetics and Genomics A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repeated sequences (‘repeats’) interspersed with short, variable sequences (‘spacers’). CRISPR arrays are transcribed and processed into individual CRISPR RNAs that each include a single spacer, and direct Cas proteins to complementary sequences in invading nucleic acid. Most bacterial CRISPR array transcripts are unusually long for untranslated RNA, suggesting the existence of mechanisms to prevent premature transcription termination by Rho, a conserved bacterial transcription termination factor that rapidly terminates untranslated RNA. We show that Rho can prematurely terminate transcription of bacterial CRISPR arrays, and we identify a widespread antitermination mechanism that antagonizes Rho to facilitate complete transcription of CRISPR arrays. Thus, our data highlight the importance of transcription termination and antitermination in the evolution of bacterial CRISPR-Cas systems. eLife Sciences Publications, Ltd 2020-10-30 /pmc/articles/PMC7665894/ /pubmed/33124980 http://dx.doi.org/10.7554/eLife.58182 Text en © 2020, Stringer et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Genetics and Genomics Stringer, Anne M Baniulyte, Gabriele Lasek-Nesselquist, Erica Seed, Kimberley D Wade, Joseph T Transcription termination and antitermination of bacterial CRISPR arrays |
title | Transcription termination and antitermination of bacterial CRISPR arrays |
title_full | Transcription termination and antitermination of bacterial CRISPR arrays |
title_fullStr | Transcription termination and antitermination of bacterial CRISPR arrays |
title_full_unstemmed | Transcription termination and antitermination of bacterial CRISPR arrays |
title_short | Transcription termination and antitermination of bacterial CRISPR arrays |
title_sort | transcription termination and antitermination of bacterial crispr arrays |
topic | Genetics and Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665894/ https://www.ncbi.nlm.nih.gov/pubmed/33124980 http://dx.doi.org/10.7554/eLife.58182 |
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