Cargando…

Comparative Analysis of Different Isolated Oleaginous Mucoromycota Fungi for Their γ-Linolenic Acid and Carotenoid Production

γ-Linolenic acid (GLA) and carotenoids have attracted much interest due to their nutraceutical and pharmaceutical importance. Mucoromycota, typical oleaginous filamentous fungi, are known for their production of valuable essential fatty acids and carotenoids. In the present study, 81 fungal strains...

Descripción completa

Detalles Bibliográficos
Autores principales: Mohamed, Hassan, El-Shanawany, Abdel-Rahim, Shah, Aabid Manzoor, Nazir, Yusuf, Naz, Tahira, Ullah, Samee, Mustafa, Kiren, Song, Yuanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665918/
https://www.ncbi.nlm.nih.gov/pubmed/33204691
http://dx.doi.org/10.1155/2020/3621543
_version_ 1783610051998515200
author Mohamed, Hassan
El-Shanawany, Abdel-Rahim
Shah, Aabid Manzoor
Nazir, Yusuf
Naz, Tahira
Ullah, Samee
Mustafa, Kiren
Song, Yuanda
author_facet Mohamed, Hassan
El-Shanawany, Abdel-Rahim
Shah, Aabid Manzoor
Nazir, Yusuf
Naz, Tahira
Ullah, Samee
Mustafa, Kiren
Song, Yuanda
author_sort Mohamed, Hassan
collection PubMed
description γ-Linolenic acid (GLA) and carotenoids have attracted much interest due to their nutraceutical and pharmaceutical importance. Mucoromycota, typical oleaginous filamentous fungi, are known for their production of valuable essential fatty acids and carotenoids. In the present study, 81 fungal strains were isolated from different Egyptian localities, out of which 11 Mucoromycota were selected for further GLA and carotenoid investigation. Comparative analysis of total lipids by GC of selected isolates showed that GLA content was the highest in Rhizomucor pusillus AUMC 11616.A, Mucor circinelloides AUMC 6696.A, and M. hiemalis AUMC 6031 that represented 0.213, 0.211, and 0.20% of CDW, respectively. Carotenoid analysis of selected isolates by spectrophotometer demonstrated that the highest yield of total carotenoids (640 μg/g) was exhibited by M. hiemalis AUMC 6031 and M. hiemalis AUMC 6695, and these isolates were found to have a similar carotenoid profile with, β-carotene (65%), zeaxanthin (34%), astaxanthin, and canthaxanthin (5%) of total carotenoids. The total fatty acids of all tested isolates showed moderate antimicrobial activity against Staphylococcus aureus and Salmonella Typhi, and Penicillium chrysogenum. To the best of our knowledge, this is the first report on the highest yield of total lipid accumulation (51.74% CDW) by a new oleaginous fungal isolate R. pusillus AUMC 11616.A. A new scope for a further study on this strain will be established to optimize and improve its total lipids with high GLA production. So, R. pusillus AUMC 11616.A might be a potential candidate for industrial application.
format Online
Article
Text
id pubmed-7665918
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-76659182020-11-16 Comparative Analysis of Different Isolated Oleaginous Mucoromycota Fungi for Their γ-Linolenic Acid and Carotenoid Production Mohamed, Hassan El-Shanawany, Abdel-Rahim Shah, Aabid Manzoor Nazir, Yusuf Naz, Tahira Ullah, Samee Mustafa, Kiren Song, Yuanda Biomed Res Int Research Article γ-Linolenic acid (GLA) and carotenoids have attracted much interest due to their nutraceutical and pharmaceutical importance. Mucoromycota, typical oleaginous filamentous fungi, are known for their production of valuable essential fatty acids and carotenoids. In the present study, 81 fungal strains were isolated from different Egyptian localities, out of which 11 Mucoromycota were selected for further GLA and carotenoid investigation. Comparative analysis of total lipids by GC of selected isolates showed that GLA content was the highest in Rhizomucor pusillus AUMC 11616.A, Mucor circinelloides AUMC 6696.A, and M. hiemalis AUMC 6031 that represented 0.213, 0.211, and 0.20% of CDW, respectively. Carotenoid analysis of selected isolates by spectrophotometer demonstrated that the highest yield of total carotenoids (640 μg/g) was exhibited by M. hiemalis AUMC 6031 and M. hiemalis AUMC 6695, and these isolates were found to have a similar carotenoid profile with, β-carotene (65%), zeaxanthin (34%), astaxanthin, and canthaxanthin (5%) of total carotenoids. The total fatty acids of all tested isolates showed moderate antimicrobial activity against Staphylococcus aureus and Salmonella Typhi, and Penicillium chrysogenum. To the best of our knowledge, this is the first report on the highest yield of total lipid accumulation (51.74% CDW) by a new oleaginous fungal isolate R. pusillus AUMC 11616.A. A new scope for a further study on this strain will be established to optimize and improve its total lipids with high GLA production. So, R. pusillus AUMC 11616.A might be a potential candidate for industrial application. Hindawi 2020-11-05 /pmc/articles/PMC7665918/ /pubmed/33204691 http://dx.doi.org/10.1155/2020/3621543 Text en Copyright © 2020 Hassan Mohamed et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mohamed, Hassan
El-Shanawany, Abdel-Rahim
Shah, Aabid Manzoor
Nazir, Yusuf
Naz, Tahira
Ullah, Samee
Mustafa, Kiren
Song, Yuanda
Comparative Analysis of Different Isolated Oleaginous Mucoromycota Fungi for Their γ-Linolenic Acid and Carotenoid Production
title Comparative Analysis of Different Isolated Oleaginous Mucoromycota Fungi for Their γ-Linolenic Acid and Carotenoid Production
title_full Comparative Analysis of Different Isolated Oleaginous Mucoromycota Fungi for Their γ-Linolenic Acid and Carotenoid Production
title_fullStr Comparative Analysis of Different Isolated Oleaginous Mucoromycota Fungi for Their γ-Linolenic Acid and Carotenoid Production
title_full_unstemmed Comparative Analysis of Different Isolated Oleaginous Mucoromycota Fungi for Their γ-Linolenic Acid and Carotenoid Production
title_short Comparative Analysis of Different Isolated Oleaginous Mucoromycota Fungi for Their γ-Linolenic Acid and Carotenoid Production
title_sort comparative analysis of different isolated oleaginous mucoromycota fungi for their γ-linolenic acid and carotenoid production
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665918/
https://www.ncbi.nlm.nih.gov/pubmed/33204691
http://dx.doi.org/10.1155/2020/3621543
work_keys_str_mv AT mohamedhassan comparativeanalysisofdifferentisolatedoleaginousmucoromycotafungifortheirglinolenicacidandcarotenoidproduction
AT elshanawanyabdelrahim comparativeanalysisofdifferentisolatedoleaginousmucoromycotafungifortheirglinolenicacidandcarotenoidproduction
AT shahaabidmanzoor comparativeanalysisofdifferentisolatedoleaginousmucoromycotafungifortheirglinolenicacidandcarotenoidproduction
AT naziryusuf comparativeanalysisofdifferentisolatedoleaginousmucoromycotafungifortheirglinolenicacidandcarotenoidproduction
AT naztahira comparativeanalysisofdifferentisolatedoleaginousmucoromycotafungifortheirglinolenicacidandcarotenoidproduction
AT ullahsamee comparativeanalysisofdifferentisolatedoleaginousmucoromycotafungifortheirglinolenicacidandcarotenoidproduction
AT mustafakiren comparativeanalysisofdifferentisolatedoleaginousmucoromycotafungifortheirglinolenicacidandcarotenoidproduction
AT songyuanda comparativeanalysisofdifferentisolatedoleaginousmucoromycotafungifortheirglinolenicacidandcarotenoidproduction