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Autologous cartilage and fibrin sealant may be superior to conventional fat grafting in preventing physeal bone bridge formation – a pilot study in porcines
PURPOSE: The article compares physeal recovery after insertion of autologous cartilage and a conventional fat graft in a standardized porcine physeal gap model. Presence of a bone bridge was the primary outcome. METHODS: Ten porcines in two groups of five were included in a paired design. A standard...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The British Editorial Society of Bone & Joint Surgery
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666797/ https://www.ncbi.nlm.nih.gov/pubmed/33204354 http://dx.doi.org/10.1302/1863-2548.14.200024 |
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author | Abood, Ahmed A. Møller-Madsen, Bjarne Shiguetomi-Medina, Juan Manuel Stødkilde-Jørgensen, Hans Foldager, Casper Rahbek, Ole |
author_facet | Abood, Ahmed A. Møller-Madsen, Bjarne Shiguetomi-Medina, Juan Manuel Stødkilde-Jørgensen, Hans Foldager, Casper Rahbek, Ole |
author_sort | Abood, Ahmed A. |
collection | PubMed |
description | PURPOSE: The article compares physeal recovery after insertion of autologous cartilage and a conventional fat graft in a standardized porcine physeal gap model. Presence of a bone bridge was the primary outcome. METHODS: Ten porcines in two groups of five were included in a paired design. A standardized physeal gap in the distal femur was made in all animals. One group (n = 5) was randomized for deposition of autologous cartilage and a Tisseel(®) or Tisseel(®) alone. The autologous cartilage was harvested from the femoral articular surface. The other group was randomized for fat grafting or no grafts at all. All animals were housed for 14 weeks. Magnetic resonance imaging (MRI) was performed at 14 weeks prior to euthanasia. The physis was harvested for histology. RESULTS: MRI – Three bone bridges were seen in the fat grafted gaps. All empty gaps formed a bone bridge. No gaps filled with autologous cartilage and Tisseel(®) resulted in bone bridges. One gap filled with Tisseel(®) only caused a bone bridge. Histology – The cartilage grafted gaps recovered with physeal-like cartilaginous tissue in histological analysis. CONCLUSIONS: Fat grafts seems ineffective in preventing bone bridges. The use of autologous cartilage may be superior to the current treatment. However, donor site complications were not investigated. The study serves as a proof of concept study and requires further investigation. LEVEL OF EVIDENCE: III |
format | Online Article Text |
id | pubmed-7666797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The British Editorial Society of Bone & Joint Surgery |
record_format | MEDLINE/PubMed |
spelling | pubmed-76667972020-11-16 Autologous cartilage and fibrin sealant may be superior to conventional fat grafting in preventing physeal bone bridge formation – a pilot study in porcines Abood, Ahmed A. Møller-Madsen, Bjarne Shiguetomi-Medina, Juan Manuel Stødkilde-Jørgensen, Hans Foldager, Casper Rahbek, Ole J Child Orthop Basic Science PURPOSE: The article compares physeal recovery after insertion of autologous cartilage and a conventional fat graft in a standardized porcine physeal gap model. Presence of a bone bridge was the primary outcome. METHODS: Ten porcines in two groups of five were included in a paired design. A standardized physeal gap in the distal femur was made in all animals. One group (n = 5) was randomized for deposition of autologous cartilage and a Tisseel(®) or Tisseel(®) alone. The autologous cartilage was harvested from the femoral articular surface. The other group was randomized for fat grafting or no grafts at all. All animals were housed for 14 weeks. Magnetic resonance imaging (MRI) was performed at 14 weeks prior to euthanasia. The physis was harvested for histology. RESULTS: MRI – Three bone bridges were seen in the fat grafted gaps. All empty gaps formed a bone bridge. No gaps filled with autologous cartilage and Tisseel(®) resulted in bone bridges. One gap filled with Tisseel(®) only caused a bone bridge. Histology – The cartilage grafted gaps recovered with physeal-like cartilaginous tissue in histological analysis. CONCLUSIONS: Fat grafts seems ineffective in preventing bone bridges. The use of autologous cartilage may be superior to the current treatment. However, donor site complications were not investigated. The study serves as a proof of concept study and requires further investigation. LEVEL OF EVIDENCE: III The British Editorial Society of Bone & Joint Surgery 2020-10-01 /pmc/articles/PMC7666797/ /pubmed/33204354 http://dx.doi.org/10.1302/1863-2548.14.200024 Text en Copyright © 2020, The author(s) http://creativecommons.org/licenses/by-nc/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (CC BY-NC 4.0) licence (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed. |
spellingShingle | Basic Science Abood, Ahmed A. Møller-Madsen, Bjarne Shiguetomi-Medina, Juan Manuel Stødkilde-Jørgensen, Hans Foldager, Casper Rahbek, Ole Autologous cartilage and fibrin sealant may be superior to conventional fat grafting in preventing physeal bone bridge formation – a pilot study in porcines |
title | Autologous cartilage and fibrin sealant may be superior to conventional fat grafting in preventing physeal bone bridge formation – a pilot study in porcines |
title_full | Autologous cartilage and fibrin sealant may be superior to conventional fat grafting in preventing physeal bone bridge formation – a pilot study in porcines |
title_fullStr | Autologous cartilage and fibrin sealant may be superior to conventional fat grafting in preventing physeal bone bridge formation – a pilot study in porcines |
title_full_unstemmed | Autologous cartilage and fibrin sealant may be superior to conventional fat grafting in preventing physeal bone bridge formation – a pilot study in porcines |
title_short | Autologous cartilage and fibrin sealant may be superior to conventional fat grafting in preventing physeal bone bridge formation – a pilot study in porcines |
title_sort | autologous cartilage and fibrin sealant may be superior to conventional fat grafting in preventing physeal bone bridge formation – a pilot study in porcines |
topic | Basic Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666797/ https://www.ncbi.nlm.nih.gov/pubmed/33204354 http://dx.doi.org/10.1302/1863-2548.14.200024 |
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