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Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
INTRODUCTION. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Instituto Nacional de Salud
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666847/ https://www.ncbi.nlm.nih.gov/pubmed/33030834 http://dx.doi.org/10.7705/biomedica.5161 |
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author | Figueiredo Pacheco, Flávia Thamiris Rodrigues Silva, Renata Kelly Novaes Souza de Carvalho, Silvia Carvalho Rocha, Felipe Trindade das Chagas, Gisele Maria Chagas Gomes, Daisy da, Hugo Medrado Ribeiro, Tereza Cristina de Mattos, Ângela Peixoto Kalabric Silva, Luciano Soares, Neci Matos Aquino Teixeira, Márcia Cristina |
author_facet | Figueiredo Pacheco, Flávia Thamiris Rodrigues Silva, Renata Kelly Novaes Souza de Carvalho, Silvia Carvalho Rocha, Felipe Trindade das Chagas, Gisele Maria Chagas Gomes, Daisy da, Hugo Medrado Ribeiro, Tereza Cristina de Mattos, Ângela Peixoto Kalabric Silva, Luciano Soares, Neci Matos Aquino Teixeira, Márcia Cristina |
author_sort | Figueiredo Pacheco, Flávia Thamiris |
collection | PubMed |
description | INTRODUCTION. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epidemiology of human giardiasis is still limited in Brazil. OBJECTIVE. To characterize G. duodenalis molecular isolates in children from Salvador, Bahia, Brazil. MATERIALS AND METHODS. Giardia duodenalis positive fecal samples were obtained from 71 children from two day care centers and 39 users of a clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and beta-giardin genes and by the sequencing of beta-giardin. RESULTS. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes. Of these, 62 (77.5 %) were identified as assemblage A and 18 (22.5%) as assemblage B. The subassemblage AII was identified in 58.8% (n=47) of isolates followed by the sub-assemblage AI (18.8%, n=15), BIV (11.2%, n=9), and BIII (5.0%, n=4). The AII sub-assemblage was the most frequent in children of both day care centers whereas AI was found only in the group attended at the clinical laboratory. Sub-assemblage AII predominated in children under two years. CONCLUSIONS. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present and a change in susceptibility to different molecular patterns of Giardia may occur during child growth. |
format | Online Article Text |
id | pubmed-7666847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Instituto Nacional de Salud |
record_format | MEDLINE/PubMed |
spelling | pubmed-76668472020-11-16 Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil Figueiredo Pacheco, Flávia Thamiris Rodrigues Silva, Renata Kelly Novaes Souza de Carvalho, Silvia Carvalho Rocha, Felipe Trindade das Chagas, Gisele Maria Chagas Gomes, Daisy da, Hugo Medrado Ribeiro, Tereza Cristina de Mattos, Ângela Peixoto Kalabric Silva, Luciano Soares, Neci Matos Aquino Teixeira, Márcia Cristina Biomedica Original Article INTRODUCTION. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epidemiology of human giardiasis is still limited in Brazil. OBJECTIVE. To characterize G. duodenalis molecular isolates in children from Salvador, Bahia, Brazil. MATERIALS AND METHODS. Giardia duodenalis positive fecal samples were obtained from 71 children from two day care centers and 39 users of a clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and beta-giardin genes and by the sequencing of beta-giardin. RESULTS. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes. Of these, 62 (77.5 %) were identified as assemblage A and 18 (22.5%) as assemblage B. The subassemblage AII was identified in 58.8% (n=47) of isolates followed by the sub-assemblage AI (18.8%, n=15), BIV (11.2%, n=9), and BIII (5.0%, n=4). The AII sub-assemblage was the most frequent in children of both day care centers whereas AI was found only in the group attended at the clinical laboratory. Sub-assemblage AII predominated in children under two years. CONCLUSIONS. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present and a change in susceptibility to different molecular patterns of Giardia may occur during child growth. Instituto Nacional de Salud 2020-06-30 /pmc/articles/PMC7666847/ /pubmed/33030834 http://dx.doi.org/10.7705/biomedica.5161 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Original Article Figueiredo Pacheco, Flávia Thamiris Rodrigues Silva, Renata Kelly Novaes Souza de Carvalho, Silvia Carvalho Rocha, Felipe Trindade das Chagas, Gisele Maria Chagas Gomes, Daisy da, Hugo Medrado Ribeiro, Tereza Cristina de Mattos, Ângela Peixoto Kalabric Silva, Luciano Soares, Neci Matos Aquino Teixeira, Márcia Cristina Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title | Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_full | Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_fullStr | Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_full_unstemmed | Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_short | Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_sort | predominance of giardia duodenalis aii sub-assemblage in young children from salvador, bahia, brazil |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666847/ https://www.ncbi.nlm.nih.gov/pubmed/33030834 http://dx.doi.org/10.7705/biomedica.5161 |
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