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Effects of TiO(2)-Coated Stainless Steel Orthodontic Wires on Streptococcus mutans Bacteria: A Clinical Study

INTRODUCTION: The aim of this study was to clinically evaluate Streptococcus mutans adhesion on titanium dioxide–coated stainless steel orthodontic wires to decrease white-spot formation. METHODS: In this study, four groups of 17 patients each (n=68) aged 12–25 years participated. A titanium dioxide...

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Autores principales: Mollabashi, Vahid, Farmany, Abbas, Alikhani, Mohammad Yousef, Sattari, Mohammad, Soltanian, Ali Reza, Kahvand, Parnian, Banisafar, Ziba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667015/
https://www.ncbi.nlm.nih.gov/pubmed/33204086
http://dx.doi.org/10.2147/IJN.S258440
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author Mollabashi, Vahid
Farmany, Abbas
Alikhani, Mohammad Yousef
Sattari, Mohammad
Soltanian, Ali Reza
Kahvand, Parnian
Banisafar, Ziba
author_facet Mollabashi, Vahid
Farmany, Abbas
Alikhani, Mohammad Yousef
Sattari, Mohammad
Soltanian, Ali Reza
Kahvand, Parnian
Banisafar, Ziba
author_sort Mollabashi, Vahid
collection PubMed
description INTRODUCTION: The aim of this study was to clinically evaluate Streptococcus mutans adhesion on titanium dioxide–coated stainless steel orthodontic wires to decrease white-spot formation. METHODS: In this study, four groups of 17 patients each (n=68) aged 12–25 years participated. A titanium dioxide coating layer was deposited on 0.4572 mm stainless steel orthodontic wires using physical vapor deposition. The coated wires were randomly assigned to one jaw, and the opposite jaw received an uncoated wire as control. Patients were divided into groups according to the duration that wires were in their mouths: A) 1 week, B) 2 weeks, C) 3 weeks, and D) 4 weeks. Block randomization was used to assign patients to each group. At the end of the experiment, 20 mm of each wire (canine-to-canine area) was cut and cultured in S. mutans–specific medium. The culture plates were placed in an incubator containing 5% CO(2) for 72 hours at 37°C, and then colonies were counted. MTT was used to test the biocompatibility of the coated and uncoated wires. To evaluate the stability of the coated titanium dioxide layer on the wires, titanium concentration on the saliva was determined using inductively coupled plasma mass spectroscopy. RESULTS: The Kruskal–Wallis test showed that there was no significant difference in colony counts among the coated wires during 1–4 weeks (p<0.48). In the uncoated-wire groups, colonys count at week 1 were higher than weeks 24 –(p<0.022). Wilcoxon’s test showed that the number of colonies was significantly different in groups A and C, but there was no significant difference in groups B or D. MTT-assay results showed that there was not a significant difference between cell viability in the coated-wire group and the control. The Kruskal–Wallis test showed that there was no significant difference in titanium concentration in the studied groups (p<0.834). CONCLUSION: Application of titanium dioxide coating is effective in reducing bacterial adhesion at wire insertion.
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spelling pubmed-76670152020-11-16 Effects of TiO(2)-Coated Stainless Steel Orthodontic Wires on Streptococcus mutans Bacteria: A Clinical Study Mollabashi, Vahid Farmany, Abbas Alikhani, Mohammad Yousef Sattari, Mohammad Soltanian, Ali Reza Kahvand, Parnian Banisafar, Ziba Int J Nanomedicine Original Research INTRODUCTION: The aim of this study was to clinically evaluate Streptococcus mutans adhesion on titanium dioxide–coated stainless steel orthodontic wires to decrease white-spot formation. METHODS: In this study, four groups of 17 patients each (n=68) aged 12–25 years participated. A titanium dioxide coating layer was deposited on 0.4572 mm stainless steel orthodontic wires using physical vapor deposition. The coated wires were randomly assigned to one jaw, and the opposite jaw received an uncoated wire as control. Patients were divided into groups according to the duration that wires were in their mouths: A) 1 week, B) 2 weeks, C) 3 weeks, and D) 4 weeks. Block randomization was used to assign patients to each group. At the end of the experiment, 20 mm of each wire (canine-to-canine area) was cut and cultured in S. mutans–specific medium. The culture plates were placed in an incubator containing 5% CO(2) for 72 hours at 37°C, and then colonies were counted. MTT was used to test the biocompatibility of the coated and uncoated wires. To evaluate the stability of the coated titanium dioxide layer on the wires, titanium concentration on the saliva was determined using inductively coupled plasma mass spectroscopy. RESULTS: The Kruskal–Wallis test showed that there was no significant difference in colony counts among the coated wires during 1–4 weeks (p<0.48). In the uncoated-wire groups, colonys count at week 1 were higher than weeks 24 –(p<0.022). Wilcoxon’s test showed that the number of colonies was significantly different in groups A and C, but there was no significant difference in groups B or D. MTT-assay results showed that there was not a significant difference between cell viability in the coated-wire group and the control. The Kruskal–Wallis test showed that there was no significant difference in titanium concentration in the studied groups (p<0.834). CONCLUSION: Application of titanium dioxide coating is effective in reducing bacterial adhesion at wire insertion. Dove 2020-11-10 /pmc/articles/PMC7667015/ /pubmed/33204086 http://dx.doi.org/10.2147/IJN.S258440 Text en © 2020 Mollabashi et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Mollabashi, Vahid
Farmany, Abbas
Alikhani, Mohammad Yousef
Sattari, Mohammad
Soltanian, Ali Reza
Kahvand, Parnian
Banisafar, Ziba
Effects of TiO(2)-Coated Stainless Steel Orthodontic Wires on Streptococcus mutans Bacteria: A Clinical Study
title Effects of TiO(2)-Coated Stainless Steel Orthodontic Wires on Streptococcus mutans Bacteria: A Clinical Study
title_full Effects of TiO(2)-Coated Stainless Steel Orthodontic Wires on Streptococcus mutans Bacteria: A Clinical Study
title_fullStr Effects of TiO(2)-Coated Stainless Steel Orthodontic Wires on Streptococcus mutans Bacteria: A Clinical Study
title_full_unstemmed Effects of TiO(2)-Coated Stainless Steel Orthodontic Wires on Streptococcus mutans Bacteria: A Clinical Study
title_short Effects of TiO(2)-Coated Stainless Steel Orthodontic Wires on Streptococcus mutans Bacteria: A Clinical Study
title_sort effects of tio(2)-coated stainless steel orthodontic wires on streptococcus mutans bacteria: a clinical study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667015/
https://www.ncbi.nlm.nih.gov/pubmed/33204086
http://dx.doi.org/10.2147/IJN.S258440
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