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Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity

The purpose of this research was to identify if Sirt3 plays a role in marrow adipogenesis and adipokines secretion, especially adiponectin using bone marrow‐derived stroma (ST2) cell model. Sirt3 overexpression leads to a significant increase in adipogenesis compared to controls. The induction of ad...

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Autores principales: Ma, Oanh, Le, Truc, Talbott, George, HoangThao Nguyen, Tram, Ha, Dorothy, Ho, Linh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667394/
https://www.ncbi.nlm.nih.gov/pubmed/33191653
http://dx.doi.org/10.1002/prp2.670
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author Ma, Oanh
Le, Truc
Talbott, George
HoangThao Nguyen, Tram
Ha, Dorothy
Ho, Linh
author_facet Ma, Oanh
Le, Truc
Talbott, George
HoangThao Nguyen, Tram
Ha, Dorothy
Ho, Linh
author_sort Ma, Oanh
collection PubMed
description The purpose of this research was to identify if Sirt3 plays a role in marrow adipogenesis and adipokines secretion, especially adiponectin using bone marrow‐derived stroma (ST2) cell model. Sirt3 overexpression leads to a significant increase in adipogenesis compared to controls. The induction of adipogenesis by Sirt3 is associated with increased gene expression of adipocyte markers as well as adiponectin/adipokines. In sharp contrast, the inhibition of Sirt3 exhibited significantly decreased adipogenesis, adipocyte markers, and adiponectin/adipokines compared to the controls. Interestingly, perilipin 1 (Plin 1) expression was decreased in Sirt3 induction but increased in Sirt3 inhibition. One hundred and fifteen mitochondrial acetylated peptides from 67 mitochondrial proteins had lower levels of acetylation in adipocytes induced by Sirt3 overexpression (Sirt3OE) compared to the control. Of the 67 proteins less enriched in acetylation, 22 acetylated proteins were decreased by more than twofold. These proteins are considered potential Sirt3 substrates in adipogenesis. In conclusion, Sirt3 has a novel, important role in modulating adipogenesis and adiponectin/adipokine expression. The connection axis among Sirt3‐adipogenesis‐adipokines was linked to its substrates by mass spectrometry analysis. These findings contribute to the efforts of revealing Sirt3 functions and Sirt3 usage as a potential target for treatment of metabolic homeostasis and diseases including type 2 diabetes.
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spelling pubmed-76673942020-11-20 Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity Ma, Oanh Le, Truc Talbott, George HoangThao Nguyen, Tram Ha, Dorothy Ho, Linh Pharmacol Res Perspect Original Articles The purpose of this research was to identify if Sirt3 plays a role in marrow adipogenesis and adipokines secretion, especially adiponectin using bone marrow‐derived stroma (ST2) cell model. Sirt3 overexpression leads to a significant increase in adipogenesis compared to controls. The induction of adipogenesis by Sirt3 is associated with increased gene expression of adipocyte markers as well as adiponectin/adipokines. In sharp contrast, the inhibition of Sirt3 exhibited significantly decreased adipogenesis, adipocyte markers, and adiponectin/adipokines compared to the controls. Interestingly, perilipin 1 (Plin 1) expression was decreased in Sirt3 induction but increased in Sirt3 inhibition. One hundred and fifteen mitochondrial acetylated peptides from 67 mitochondrial proteins had lower levels of acetylation in adipocytes induced by Sirt3 overexpression (Sirt3OE) compared to the control. Of the 67 proteins less enriched in acetylation, 22 acetylated proteins were decreased by more than twofold. These proteins are considered potential Sirt3 substrates in adipogenesis. In conclusion, Sirt3 has a novel, important role in modulating adipogenesis and adiponectin/adipokine expression. The connection axis among Sirt3‐adipogenesis‐adipokines was linked to its substrates by mass spectrometry analysis. These findings contribute to the efforts of revealing Sirt3 functions and Sirt3 usage as a potential target for treatment of metabolic homeostasis and diseases including type 2 diabetes. John Wiley and Sons Inc. 2020-11-15 /pmc/articles/PMC7667394/ /pubmed/33191653 http://dx.doi.org/10.1002/prp2.670 Text en © 2020 The Authors. Pharmacology Research & Perspectives published by John Wiley & Sons Ltd, British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Ma, Oanh
Le, Truc
Talbott, George
HoangThao Nguyen, Tram
Ha, Dorothy
Ho, Linh
Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
title Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
title_full Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
title_fullStr Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
title_full_unstemmed Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
title_short Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
title_sort sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667394/
https://www.ncbi.nlm.nih.gov/pubmed/33191653
http://dx.doi.org/10.1002/prp2.670
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