Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions

Site-directed spin labeling (SDSL) of large RNAs for electron paramagnetic resonance (EPR) spectroscopy has remained challenging to date. We here demonstrate an efficient and generally applicable posttranscriptional SDSL method for large RNAs using an expanded genetic alphabet containing the NaM-TPT...

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Detalles Bibliográficos
Autores principales: Wang, Yan, Kathiresan, Venkatesan, Chen, Yaoyi, Hu, Yanping, Jiang, Wei, Bai, Guangcan, Liu, Guoquan, Qin, Peter Z., Fang, Xianyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667596/
https://www.ncbi.nlm.nih.gov/pubmed/33224460
http://dx.doi.org/10.1039/d0sc01717e

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