Cargando…

Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China

BACKGROUND: Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. The coypu (Myocastor coypus) is a semi-aquatic rodent, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown....

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Fuchang, Cao, Yangwenna, Wang, Haiyan, Liu, Qiang, Zhao, Aiyun, Qi, Meng, Zhang, Longxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667729/
https://www.ncbi.nlm.nih.gov/pubmed/33198788
http://dx.doi.org/10.1186/s13071-020-04436-0
_version_ 1783610370069364736
author Yu, Fuchang
Cao, Yangwenna
Wang, Haiyan
Liu, Qiang
Zhao, Aiyun
Qi, Meng
Zhang, Longxian
author_facet Yu, Fuchang
Cao, Yangwenna
Wang, Haiyan
Liu, Qiang
Zhao, Aiyun
Qi, Meng
Zhang, Longxian
author_sort Yu, Fuchang
collection PubMed
description BACKGROUND: Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. The coypu (Myocastor coypus) is a semi-aquatic rodent, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown. METHODS: A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. RESULTS: Enterocytozoon bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes (CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7)) and a novel genotype named CNCP1 (n = 1). CONCLUSIONS: The rare genotype CHN4 was the most common genotype in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. To the best of our knowledge, this is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be a potential infection source to humans. [Image: see text]
format Online
Article
Text
id pubmed-7667729
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-76677292020-11-17 Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China Yu, Fuchang Cao, Yangwenna Wang, Haiyan Liu, Qiang Zhao, Aiyun Qi, Meng Zhang, Longxian Parasit Vectors Research BACKGROUND: Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. The coypu (Myocastor coypus) is a semi-aquatic rodent, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown. METHODS: A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. RESULTS: Enterocytozoon bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes (CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7)) and a novel genotype named CNCP1 (n = 1). CONCLUSIONS: The rare genotype CHN4 was the most common genotype in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. To the best of our knowledge, this is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be a potential infection source to humans. [Image: see text] BioMed Central 2020-11-16 /pmc/articles/PMC7667729/ /pubmed/33198788 http://dx.doi.org/10.1186/s13071-020-04436-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Yu, Fuchang
Cao, Yangwenna
Wang, Haiyan
Liu, Qiang
Zhao, Aiyun
Qi, Meng
Zhang, Longxian
Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China
title Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China
title_full Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China
title_fullStr Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China
title_full_unstemmed Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China
title_short Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China
title_sort host-adaptation of the rare enterocytozoon bieneusi genotype chn4 in myocastor coypus (rodentia: echimyidae) in china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667729/
https://www.ncbi.nlm.nih.gov/pubmed/33198788
http://dx.doi.org/10.1186/s13071-020-04436-0
work_keys_str_mv AT yufuchang hostadaptationoftherareenterocytozoonbieneusigenotypechn4inmyocastorcoypusrodentiaechimyidaeinchina
AT caoyangwenna hostadaptationoftherareenterocytozoonbieneusigenotypechn4inmyocastorcoypusrodentiaechimyidaeinchina
AT wanghaiyan hostadaptationoftherareenterocytozoonbieneusigenotypechn4inmyocastorcoypusrodentiaechimyidaeinchina
AT liuqiang hostadaptationoftherareenterocytozoonbieneusigenotypechn4inmyocastorcoypusrodentiaechimyidaeinchina
AT zhaoaiyun hostadaptationoftherareenterocytozoonbieneusigenotypechn4inmyocastorcoypusrodentiaechimyidaeinchina
AT qimeng hostadaptationoftherareenterocytozoonbieneusigenotypechn4inmyocastorcoypusrodentiaechimyidaeinchina
AT zhanglongxian hostadaptationoftherareenterocytozoonbieneusigenotypechn4inmyocastorcoypusrodentiaechimyidaeinchina