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Sperm miRNAs— potential mediators of bull age and early embryo development

BACKGROUND: Sperm miRNAs were reported to regulate spermatogenesis and early embryonic development in some mammals including bovine. The dairy cattle breeding industry now tends to collect semen from younger bulls under high selection pressure at a time when semen quality may be suboptimal compared...

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Autores principales: Wu, Chongyang, Blondin, Patrick, Vigneault, Christian, Labrecque, Rémi, Sirard, Marc-André
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667858/
https://www.ncbi.nlm.nih.gov/pubmed/33198638
http://dx.doi.org/10.1186/s12864-020-07206-5
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author Wu, Chongyang
Blondin, Patrick
Vigneault, Christian
Labrecque, Rémi
Sirard, Marc-André
author_facet Wu, Chongyang
Blondin, Patrick
Vigneault, Christian
Labrecque, Rémi
Sirard, Marc-André
author_sort Wu, Chongyang
collection PubMed
description BACKGROUND: Sperm miRNAs were reported to regulate spermatogenesis and early embryonic development in some mammals including bovine. The dairy cattle breeding industry now tends to collect semen from younger bulls under high selection pressure at a time when semen quality may be suboptimal compared to adult bulls. Whether the patterns of spermatic miRNAs are affected by paternal age and/or impact early embryogenesis is not clear. Hence, we generated small non-coding RNA libraries of sperm collected from same bulls at 10, 12, and 16 months of age, using 16 months as control for differential expression and functional analysis. RESULTS: We firstly excluded all miRNAs present in measurable quantity in oocytes according to the literature. Of the remaining miRNAs, ten sperm-borne miRNAs were significantly differentially expressed in younger bulls (four in the 10 vs 16 months contrast and six in the 12 vs 16 months contrast). Targets of miRNAs were identified and compared to the transcriptomic database of two-cell embryos, to genes related to two-cell competence, and to the transcriptomic database of blastocysts. Ingenuity pathway analysis of the targets of these miRNAs suggested potential influence on the developmental competence of two-cell embryos and on metabolism and protein synthesis in blastocysts. CONCLUSIONS: The results showed that miRNA patterns in sperm are affected by the age of the bull and may mediate the effects of paternal age on early embryonic development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-020-07206-5.
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spelling pubmed-76678582020-11-17 Sperm miRNAs— potential mediators of bull age and early embryo development Wu, Chongyang Blondin, Patrick Vigneault, Christian Labrecque, Rémi Sirard, Marc-André BMC Genomics Research Article BACKGROUND: Sperm miRNAs were reported to regulate spermatogenesis and early embryonic development in some mammals including bovine. The dairy cattle breeding industry now tends to collect semen from younger bulls under high selection pressure at a time when semen quality may be suboptimal compared to adult bulls. Whether the patterns of spermatic miRNAs are affected by paternal age and/or impact early embryogenesis is not clear. Hence, we generated small non-coding RNA libraries of sperm collected from same bulls at 10, 12, and 16 months of age, using 16 months as control for differential expression and functional analysis. RESULTS: We firstly excluded all miRNAs present in measurable quantity in oocytes according to the literature. Of the remaining miRNAs, ten sperm-borne miRNAs were significantly differentially expressed in younger bulls (four in the 10 vs 16 months contrast and six in the 12 vs 16 months contrast). Targets of miRNAs were identified and compared to the transcriptomic database of two-cell embryos, to genes related to two-cell competence, and to the transcriptomic database of blastocysts. Ingenuity pathway analysis of the targets of these miRNAs suggested potential influence on the developmental competence of two-cell embryos and on metabolism and protein synthesis in blastocysts. CONCLUSIONS: The results showed that miRNA patterns in sperm are affected by the age of the bull and may mediate the effects of paternal age on early embryonic development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-020-07206-5. BioMed Central 2020-11-16 /pmc/articles/PMC7667858/ /pubmed/33198638 http://dx.doi.org/10.1186/s12864-020-07206-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Wu, Chongyang
Blondin, Patrick
Vigneault, Christian
Labrecque, Rémi
Sirard, Marc-André
Sperm miRNAs— potential mediators of bull age and early embryo development
title Sperm miRNAs— potential mediators of bull age and early embryo development
title_full Sperm miRNAs— potential mediators of bull age and early embryo development
title_fullStr Sperm miRNAs— potential mediators of bull age and early embryo development
title_full_unstemmed Sperm miRNAs— potential mediators of bull age and early embryo development
title_short Sperm miRNAs— potential mediators of bull age and early embryo development
title_sort sperm mirnas— potential mediators of bull age and early embryo development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667858/
https://www.ncbi.nlm.nih.gov/pubmed/33198638
http://dx.doi.org/10.1186/s12864-020-07206-5
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