IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in China. N6−methyladenosine (m6A) plays an important role in posttranscriptional gene regulation. METTL3 and IGF2BP2 are key genes in the m6A signal pathway and have recently been shown to play important roles in cancer devel...

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Autores principales: Pu, Jian, Wang, Jianchu, Qin, Zebang, Wang, Anmin, Zhang, Ya, Wu, Xianjian, Wu, Yi, Li, Wenchuan, Xu, Zuoming, Lu, Yuan, Tang, Qianli, Wei, Huamei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Oncology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667992/
https://www.ncbi.nlm.nih.gov/pubmed/33224879
http://dx.doi.org/10.3389/fonc.2020.578816
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author Pu, Jian
Wang, Jianchu
Qin, Zebang
Wang, Anmin
Zhang, Ya
Wu, Xianjian
Wu, Yi
Li, Wenchuan
Xu, Zuoming
Lu, Yuan
Tang, Qianli
Wei, Huamei
author_facet Pu, Jian
Wang, Jianchu
Qin, Zebang
Wang, Anmin
Zhang, Ya
Wu, Xianjian
Wu, Yi
Li, Wenchuan
Xu, Zuoming
Lu, Yuan
Tang, Qianli
Wei, Huamei
author_sort Pu, Jian
collection PubMed
description Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in China. N6−methyladenosine (m6A) plays an important role in posttranscriptional gene regulation. METTL3 and IGF2BP2 are key genes in the m6A signal pathway and have recently been shown to play important roles in cancer development and progression. In our work, higher METTL3 and IGF2BP2 expression were found in HCC tissues and were associated with a poor prognosis. In addition, IGF2BP2 overexpression promoted HCC proliferation in vitro and in vivo. Mechanistically, IGF2BP2 directly recognized and bound to the m6A site on FEN1 mRNA and enhanced FEN1 mRNA stability. Overall, our study revealed that METTL3 and IGF2BP2, acting as an oncogene, maintained FEN1 expression through an m6A-IGF2BP2-dependent mechanism in HCC cells, and indicated a potential biomarker panel for prognostic prediction in liver cancer.
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spelling pubmed-76679922020-11-20 IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism Pu, Jian Wang, Jianchu Qin, Zebang Wang, Anmin Zhang, Ya Wu, Xianjian Wu, Yi Li, Wenchuan Xu, Zuoming Lu, Yuan Tang, Qianli Wei, Huamei Front Oncol Oncology Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in China. N6−methyladenosine (m6A) plays an important role in posttranscriptional gene regulation. METTL3 and IGF2BP2 are key genes in the m6A signal pathway and have recently been shown to play important roles in cancer development and progression. In our work, higher METTL3 and IGF2BP2 expression were found in HCC tissues and were associated with a poor prognosis. In addition, IGF2BP2 overexpression promoted HCC proliferation in vitro and in vivo. Mechanistically, IGF2BP2 directly recognized and bound to the m6A site on FEN1 mRNA and enhanced FEN1 mRNA stability. Overall, our study revealed that METTL3 and IGF2BP2, acting as an oncogene, maintained FEN1 expression through an m6A-IGF2BP2-dependent mechanism in HCC cells, and indicated a potential biomarker panel for prognostic prediction in liver cancer. Frontiers Media S.A. 2020-11-02 /pmc/articles/PMC7667992/ /pubmed/33224879 http://dx.doi.org/10.3389/fonc.2020.578816 Text en Copyright © 2020 Pu, Wang, Qin, Wang, Zhang, Wu, Wu, Li, Xu, Lu, Tang and Wei http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Pu, Jian
Wang, Jianchu
Qin, Zebang
Wang, Anmin
Zhang, Ya
Wu, Xianjian
Wu, Yi
Li, Wenchuan
Xu, Zuoming
Lu, Yuan
Tang, Qianli
Wei, Huamei
IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism
title IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism
title_full IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism
title_fullStr IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism
title_full_unstemmed IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism
title_short IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism
title_sort igf2bp2 promotes liver cancer growth through an m6a-fen1-dependent mechanism
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667992/
https://www.ncbi.nlm.nih.gov/pubmed/33224879
http://dx.doi.org/10.3389/fonc.2020.578816
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