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Nontuberculous mycobacteria – clinical and laboratory diagnosis: experiences from a TB endemic country

AIM: To evaluate the performance of VITEK(®)MS with DNA sequencing for laboratory diagnosis of non-tuberculous mycobacteria (NTM) species in a resource-limited setting. METHODS: 16SrRNA sequencing and MALDI-TOF mass spectrometry (VITEK(®)MS) was performed at a tertiary-care hospital in India. MALDI-...

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Detalles Bibliográficos
Autores principales: Sam, Ann Susan, Ninan, Marilyn Mary, Ranjani, R, Devanga Raghupathi, Naveen Kumar, Balaji, Veeraraghavan, Michael, Joy Sarojini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668123/
https://www.ncbi.nlm.nih.gov/pubmed/33235807
http://dx.doi.org/10.2144/fsoa-2020-0023
Descripción
Sumario:AIM: To evaluate the performance of VITEK(®)MS with DNA sequencing for laboratory diagnosis of non-tuberculous mycobacteria (NTM) species in a resource-limited setting. METHODS: 16SrRNA sequencing and MALDI-TOF mass spectrometry (VITEK(®)MS) was performed at a tertiary-care hospital in India. MALDI-TOF results were confirmed by 16S rRNA sequencing. In addition, sequencing of the internal transcribed spacer region was performed on slowly growing NTM. RESULTS: Commonest species isolated were M. abscessus, M. intracellulare, M. avium, M. fortuitum and M. simiae. 16S rRNA sequencing and MALDI-TOF results had agreement of 94.5% for rapidly growing and 77.5% for slowly growing NTM. CONCLUSION: There is good correlation between VITEK(®)MS and sequencing for rapidly growing NTM. For slowly growing species, sequencing would be required in a third isolates.