Cargando…
Nontuberculous mycobacteria – clinical and laboratory diagnosis: experiences from a TB endemic country
AIM: To evaluate the performance of VITEK(®)MS with DNA sequencing for laboratory diagnosis of non-tuberculous mycobacteria (NTM) species in a resource-limited setting. METHODS: 16SrRNA sequencing and MALDI-TOF mass spectrometry (VITEK(®)MS) was performed at a tertiary-care hospital in India. MALDI-...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668123/ https://www.ncbi.nlm.nih.gov/pubmed/33235807 http://dx.doi.org/10.2144/fsoa-2020-0023 |
Sumario: | AIM: To evaluate the performance of VITEK(®)MS with DNA sequencing for laboratory diagnosis of non-tuberculous mycobacteria (NTM) species in a resource-limited setting. METHODS: 16SrRNA sequencing and MALDI-TOF mass spectrometry (VITEK(®)MS) was performed at a tertiary-care hospital in India. MALDI-TOF results were confirmed by 16S rRNA sequencing. In addition, sequencing of the internal transcribed spacer region was performed on slowly growing NTM. RESULTS: Commonest species isolated were M. abscessus, M. intracellulare, M. avium, M. fortuitum and M. simiae. 16S rRNA sequencing and MALDI-TOF results had agreement of 94.5% for rapidly growing and 77.5% for slowly growing NTM. CONCLUSION: There is good correlation between VITEK(®)MS and sequencing for rapidly growing NTM. For slowly growing species, sequencing would be required in a third isolates. |
---|