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Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion (Neophoca cinerea) and other mammals

Measurement of cytokine gene expression by reverse transcription quantitative polymerase chain reaction (RT-qPCR) is used widely to assess the immune system of animals and to identify biomarkers of disease, but its application is limited in wildlife species due to a lack of species-specific reagents...

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Detalles Bibliográficos
Autores principales: Meza Cerda, María-Ignacia, Gray, Rachael, Higgins, Damien P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668205/
https://www.ncbi.nlm.nih.gov/pubmed/33240637
http://dx.doi.org/10.7717/peerj.10306