Gain-of-function assay for SARS-CoV-2 M(pro) inhibition in living cells

The main protease, M(pro), of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M(pro) function in living cells, in which protease inhibition by genetic or chemical methods resu...

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Detalles Bibliográficos
Autores principales: Moghadasi, Seyad Arad, Becker, Jordan T., Belica, Christopher, Wick, Chloe, Brown, William L., Harris, Reuben S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668733/
https://www.ncbi.nlm.nih.gov/pubmed/33200129
http://dx.doi.org/10.1101/2020.11.09.375139
Descripción
Sumario:The main protease, M(pro), of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M(pro) function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing.

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