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Single nuclei RNA-seq of mouse placental labyrinth development
The placenta is the interface between mother and fetus in all eutherian species. However, our understanding of this essential organ remains incomplete. A substantial challenge has been the syncytial cells of the placenta, which have made dissociation and independent evaluation of the different cell...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669270/ https://www.ncbi.nlm.nih.gov/pubmed/33141023 http://dx.doi.org/10.7554/eLife.60266 |
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author | Marsh, Bryan Blelloch, Robert |
author_facet | Marsh, Bryan Blelloch, Robert |
author_sort | Marsh, Bryan |
collection | PubMed |
description | The placenta is the interface between mother and fetus in all eutherian species. However, our understanding of this essential organ remains incomplete. A substantial challenge has been the syncytial cells of the placenta, which have made dissociation and independent evaluation of the different cell types of this organ difficult. Here, we address questions concerning the ontogeny, specification, and function of the cell types of a representative hemochorial placenta by performing single nuclei RNA sequencing (snRNA-seq) at multiple stages of mouse embryonic development focusing on the exchange interface, the labyrinth. Timepoints extended from progenitor-driven expansion through terminal differentiation. Analysis by snRNA-seq identified transcript profiles and inferred functions, cell trajectories, signaling interactions, and transcriptional drivers of all but the most highly polyploid cell types of the placenta. These data profile placental development at an unprecedented resolution, provide insights into differentiation and function across time, and provide a resource for future study. |
format | Online Article Text |
id | pubmed-7669270 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-76692702020-11-18 Single nuclei RNA-seq of mouse placental labyrinth development Marsh, Bryan Blelloch, Robert eLife Developmental Biology The placenta is the interface between mother and fetus in all eutherian species. However, our understanding of this essential organ remains incomplete. A substantial challenge has been the syncytial cells of the placenta, which have made dissociation and independent evaluation of the different cell types of this organ difficult. Here, we address questions concerning the ontogeny, specification, and function of the cell types of a representative hemochorial placenta by performing single nuclei RNA sequencing (snRNA-seq) at multiple stages of mouse embryonic development focusing on the exchange interface, the labyrinth. Timepoints extended from progenitor-driven expansion through terminal differentiation. Analysis by snRNA-seq identified transcript profiles and inferred functions, cell trajectories, signaling interactions, and transcriptional drivers of all but the most highly polyploid cell types of the placenta. These data profile placental development at an unprecedented resolution, provide insights into differentiation and function across time, and provide a resource for future study. eLife Sciences Publications, Ltd 2020-11-03 /pmc/articles/PMC7669270/ /pubmed/33141023 http://dx.doi.org/10.7554/eLife.60266 Text en © 2020, Marsh and Blelloch http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Marsh, Bryan Blelloch, Robert Single nuclei RNA-seq of mouse placental labyrinth development |
title | Single nuclei RNA-seq of mouse placental labyrinth development |
title_full | Single nuclei RNA-seq of mouse placental labyrinth development |
title_fullStr | Single nuclei RNA-seq of mouse placental labyrinth development |
title_full_unstemmed | Single nuclei RNA-seq of mouse placental labyrinth development |
title_short | Single nuclei RNA-seq of mouse placental labyrinth development |
title_sort | single nuclei rna-seq of mouse placental labyrinth development |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669270/ https://www.ncbi.nlm.nih.gov/pubmed/33141023 http://dx.doi.org/10.7554/eLife.60266 |
work_keys_str_mv | AT marshbryan singlenucleirnaseqofmouseplacentallabyrinthdevelopment AT blellochrobert singlenucleirnaseqofmouseplacentallabyrinthdevelopment |