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Single nuclei RNA-seq of mouse placental labyrinth development

The placenta is the interface between mother and fetus in all eutherian species. However, our understanding of this essential organ remains incomplete. A substantial challenge has been the syncytial cells of the placenta, which have made dissociation and independent evaluation of the different cell...

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Detalles Bibliográficos
Autores principales: Marsh, Bryan, Blelloch, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669270/
https://www.ncbi.nlm.nih.gov/pubmed/33141023
http://dx.doi.org/10.7554/eLife.60266
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author Marsh, Bryan
Blelloch, Robert
author_facet Marsh, Bryan
Blelloch, Robert
author_sort Marsh, Bryan
collection PubMed
description The placenta is the interface between mother and fetus in all eutherian species. However, our understanding of this essential organ remains incomplete. A substantial challenge has been the syncytial cells of the placenta, which have made dissociation and independent evaluation of the different cell types of this organ difficult. Here, we address questions concerning the ontogeny, specification, and function of the cell types of a representative hemochorial placenta by performing single nuclei RNA sequencing (snRNA-seq) at multiple stages of mouse embryonic development focusing on the exchange interface, the labyrinth. Timepoints extended from progenitor-driven expansion through terminal differentiation. Analysis by snRNA-seq identified transcript profiles and inferred functions, cell trajectories, signaling interactions, and transcriptional drivers of all but the most highly polyploid cell types of the placenta. These data profile placental development at an unprecedented resolution, provide insights into differentiation and function across time, and provide a resource for future study.
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spelling pubmed-76692702020-11-18 Single nuclei RNA-seq of mouse placental labyrinth development Marsh, Bryan Blelloch, Robert eLife Developmental Biology The placenta is the interface between mother and fetus in all eutherian species. However, our understanding of this essential organ remains incomplete. A substantial challenge has been the syncytial cells of the placenta, which have made dissociation and independent evaluation of the different cell types of this organ difficult. Here, we address questions concerning the ontogeny, specification, and function of the cell types of a representative hemochorial placenta by performing single nuclei RNA sequencing (snRNA-seq) at multiple stages of mouse embryonic development focusing on the exchange interface, the labyrinth. Timepoints extended from progenitor-driven expansion through terminal differentiation. Analysis by snRNA-seq identified transcript profiles and inferred functions, cell trajectories, signaling interactions, and transcriptional drivers of all but the most highly polyploid cell types of the placenta. These data profile placental development at an unprecedented resolution, provide insights into differentiation and function across time, and provide a resource for future study. eLife Sciences Publications, Ltd 2020-11-03 /pmc/articles/PMC7669270/ /pubmed/33141023 http://dx.doi.org/10.7554/eLife.60266 Text en © 2020, Marsh and Blelloch http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Marsh, Bryan
Blelloch, Robert
Single nuclei RNA-seq of mouse placental labyrinth development
title Single nuclei RNA-seq of mouse placental labyrinth development
title_full Single nuclei RNA-seq of mouse placental labyrinth development
title_fullStr Single nuclei RNA-seq of mouse placental labyrinth development
title_full_unstemmed Single nuclei RNA-seq of mouse placental labyrinth development
title_short Single nuclei RNA-seq of mouse placental labyrinth development
title_sort single nuclei rna-seq of mouse placental labyrinth development
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669270/
https://www.ncbi.nlm.nih.gov/pubmed/33141023
http://dx.doi.org/10.7554/eLife.60266
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