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Persistence of pdm2009-H1N1 internal genes of swine influenza in pigs, Thailand
Swine influenza is one of the important zoonotic diseases of pigs. We conducted a longitudinal survey of swine influenza A viruses (S-IAV) circulating in a pig farm with history of endemic S-IAV infection from 2017 to 2018. The samples were collected from 436 pigs including nasal swab samples (n = 4...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669897/ https://www.ncbi.nlm.nih.gov/pubmed/33199784 http://dx.doi.org/10.1038/s41598-020-76771-2 |
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author | Nasamran, Chanakarn Janetanakit, Taveesak Chiyawong, Supasama Boonyapisitsopa, Supanat Bunpapong, Napawan Prakairungnamthip, Duangduean Thontiravong, Aunyaratana Amonsin, Alongkorn |
author_facet | Nasamran, Chanakarn Janetanakit, Taveesak Chiyawong, Supasama Boonyapisitsopa, Supanat Bunpapong, Napawan Prakairungnamthip, Duangduean Thontiravong, Aunyaratana Amonsin, Alongkorn |
author_sort | Nasamran, Chanakarn |
collection | PubMed |
description | Swine influenza is one of the important zoonotic diseases of pigs. We conducted a longitudinal survey of swine influenza A viruses (S-IAV) circulating in a pig farm with history of endemic S-IAV infection from 2017 to 2018. The samples were collected from 436 pigs including nasal swab samples (n = 436) and blood samples (n = 436). Our result showed that 18.81% (82/436) were positive for influenza A virus and subsequently 57 S-IAV could be isolated. Then 24 out of 57 S-IAVs were selected for whole genome sequencing and could be subtyped as S-IAV-H1N1 (n = 18) and S-IAV-H3N2 (n = 6). Of 24 S-IAVs, we observed 3 genotypes of S-IAVs including rH1N1 (pdm + 1), rH1N1 (pdm + 2), and rH3N2 (pdm + 2). Since all genotypes of S-IAVs in this study contained internal genes from pdmH1N1-2009, it could be speculated that pdmH1N1-2009 was introduced in a pig farm and then multiple reassorted with endemic S-IAVs to generate diversify S-IAV genotypes. Our study supported and added the evidences that pdmH1N1-2009 and it reassortant have predominately persisted in pig population in Thailand. Thus, monitoring of S-IAVs in pigs, farm workers and veterinarians in pig farms is important and should be routinely conducted. |
format | Online Article Text |
id | pubmed-7669897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76698972020-11-18 Persistence of pdm2009-H1N1 internal genes of swine influenza in pigs, Thailand Nasamran, Chanakarn Janetanakit, Taveesak Chiyawong, Supasama Boonyapisitsopa, Supanat Bunpapong, Napawan Prakairungnamthip, Duangduean Thontiravong, Aunyaratana Amonsin, Alongkorn Sci Rep Article Swine influenza is one of the important zoonotic diseases of pigs. We conducted a longitudinal survey of swine influenza A viruses (S-IAV) circulating in a pig farm with history of endemic S-IAV infection from 2017 to 2018. The samples were collected from 436 pigs including nasal swab samples (n = 436) and blood samples (n = 436). Our result showed that 18.81% (82/436) were positive for influenza A virus and subsequently 57 S-IAV could be isolated. Then 24 out of 57 S-IAVs were selected for whole genome sequencing and could be subtyped as S-IAV-H1N1 (n = 18) and S-IAV-H3N2 (n = 6). Of 24 S-IAVs, we observed 3 genotypes of S-IAVs including rH1N1 (pdm + 1), rH1N1 (pdm + 2), and rH3N2 (pdm + 2). Since all genotypes of S-IAVs in this study contained internal genes from pdmH1N1-2009, it could be speculated that pdmH1N1-2009 was introduced in a pig farm and then multiple reassorted with endemic S-IAVs to generate diversify S-IAV genotypes. Our study supported and added the evidences that pdmH1N1-2009 and it reassortant have predominately persisted in pig population in Thailand. Thus, monitoring of S-IAVs in pigs, farm workers and veterinarians in pig farms is important and should be routinely conducted. Nature Publishing Group UK 2020-11-16 /pmc/articles/PMC7669897/ /pubmed/33199784 http://dx.doi.org/10.1038/s41598-020-76771-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nasamran, Chanakarn Janetanakit, Taveesak Chiyawong, Supasama Boonyapisitsopa, Supanat Bunpapong, Napawan Prakairungnamthip, Duangduean Thontiravong, Aunyaratana Amonsin, Alongkorn Persistence of pdm2009-H1N1 internal genes of swine influenza in pigs, Thailand |
title | Persistence of pdm2009-H1N1 internal genes of swine influenza in pigs, Thailand |
title_full | Persistence of pdm2009-H1N1 internal genes of swine influenza in pigs, Thailand |
title_fullStr | Persistence of pdm2009-H1N1 internal genes of swine influenza in pigs, Thailand |
title_full_unstemmed | Persistence of pdm2009-H1N1 internal genes of swine influenza in pigs, Thailand |
title_short | Persistence of pdm2009-H1N1 internal genes of swine influenza in pigs, Thailand |
title_sort | persistence of pdm2009-h1n1 internal genes of swine influenza in pigs, thailand |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669897/ https://www.ncbi.nlm.nih.gov/pubmed/33199784 http://dx.doi.org/10.1038/s41598-020-76771-2 |
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