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The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms
Aureochromes represent a unique type of blue light photoreceptors that possess a blue light sensing flavin-binding LOV-domain and a DNA-binding bZIP domain, thus being light-driven transcription factors. The diatom Phaeodactylum tricornutum, a member of the essential marine primary producers, posses...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670200/ https://www.ncbi.nlm.nih.gov/pubmed/33235981 http://dx.doi.org/10.1016/j.isci.2020.101730 |
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author | Mann, Marcus Serif, Manuel Wrobel, Thomas Eisenhut, Marion Madhuri, Shvaita Flachbart, Samantha Weber, Andreas P.M. Lepetit, Bernard Wilhelm, Christian Kroth, Peter G. |
author_facet | Mann, Marcus Serif, Manuel Wrobel, Thomas Eisenhut, Marion Madhuri, Shvaita Flachbart, Samantha Weber, Andreas P.M. Lepetit, Bernard Wilhelm, Christian Kroth, Peter G. |
author_sort | Mann, Marcus |
collection | PubMed |
description | Aureochromes represent a unique type of blue light photoreceptors that possess a blue light sensing flavin-binding LOV-domain and a DNA-binding bZIP domain, thus being light-driven transcription factors. The diatom Phaeodactylum tricornutum, a member of the essential marine primary producers, possesses four aureochromes (PtAUREO1a, 1b, 1c, 2). Here we show a dramatic change in the global gene expression pattern of P. tricornutum wild-type cells after a shift from red to blue light. About 75% of the genes show significantly changed transcript levels already after 10 and 60 min of blue light exposure, which includes genes of major transcription factors as well as other photoreceptors. Very surprisingly, this light-induced regulation of gene expression is almost completely inhibited in independent PtAureo1a knockout lines. Such a massive and fast transcriptional change depending on one single photoreceptor is so far unprecedented. We conclude that PtAUREO1a plays a key role in diatoms upon blue light exposure. |
format | Online Article Text |
id | pubmed-7670200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-76702002020-11-23 The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms Mann, Marcus Serif, Manuel Wrobel, Thomas Eisenhut, Marion Madhuri, Shvaita Flachbart, Samantha Weber, Andreas P.M. Lepetit, Bernard Wilhelm, Christian Kroth, Peter G. iScience Article Aureochromes represent a unique type of blue light photoreceptors that possess a blue light sensing flavin-binding LOV-domain and a DNA-binding bZIP domain, thus being light-driven transcription factors. The diatom Phaeodactylum tricornutum, a member of the essential marine primary producers, possesses four aureochromes (PtAUREO1a, 1b, 1c, 2). Here we show a dramatic change in the global gene expression pattern of P. tricornutum wild-type cells after a shift from red to blue light. About 75% of the genes show significantly changed transcript levels already after 10 and 60 min of blue light exposure, which includes genes of major transcription factors as well as other photoreceptors. Very surprisingly, this light-induced regulation of gene expression is almost completely inhibited in independent PtAureo1a knockout lines. Such a massive and fast transcriptional change depending on one single photoreceptor is so far unprecedented. We conclude that PtAUREO1a plays a key role in diatoms upon blue light exposure. Elsevier 2020-10-24 /pmc/articles/PMC7670200/ /pubmed/33235981 http://dx.doi.org/10.1016/j.isci.2020.101730 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mann, Marcus Serif, Manuel Wrobel, Thomas Eisenhut, Marion Madhuri, Shvaita Flachbart, Samantha Weber, Andreas P.M. Lepetit, Bernard Wilhelm, Christian Kroth, Peter G. The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms |
title | The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms |
title_full | The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms |
title_fullStr | The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms |
title_full_unstemmed | The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms |
title_short | The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms |
title_sort | aureochrome photoreceptor ptaureo1a is a highly effective blue light switch in diatoms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670200/ https://www.ncbi.nlm.nih.gov/pubmed/33235981 http://dx.doi.org/10.1016/j.isci.2020.101730 |
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