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Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress

BACKGROUND: For microorganisms on a paper surface, the lack of water is one of the most important stress factors. A strain of Bacillus megaterium FDU301 was isolated from plaques on a paper surface using culture medium with polyethylene glycol 200 (PEG200) to simulate an arid condition. Global trans...

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Autores principales: Zhao, Lei, Zhou, Yanjun, Li, Jianbei, Xia, Yucheng, Wang, Weiyun, Luo, Xiuqi, Yin, Juan, Zhong, Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670681/
https://www.ncbi.nlm.nih.gov/pubmed/33198631
http://dx.doi.org/10.1186/s12866-020-02039-4
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author Zhao, Lei
Zhou, Yanjun
Li, Jianbei
Xia, Yucheng
Wang, Weiyun
Luo, Xiuqi
Yin, Juan
Zhong, Jiang
author_facet Zhao, Lei
Zhou, Yanjun
Li, Jianbei
Xia, Yucheng
Wang, Weiyun
Luo, Xiuqi
Yin, Juan
Zhong, Jiang
author_sort Zhao, Lei
collection PubMed
description BACKGROUND: For microorganisms on a paper surface, the lack of water is one of the most important stress factors. A strain of Bacillus megaterium FDU301 was isolated from plaques on a paper surface using culture medium with polyethylene glycol 200 (PEG200) to simulate an arid condition. Global transcriptomic analysis of B. megaterium FDU301 grown under normal and simulated arid conditions was performed via RNA-seq technology to identify genes involved in arid stress adaptation. RESULTS: The transcriptome of B. megaterium FDU301 grown in LB medium under arid (15% PEG200 (w/w)) and normal conditions were compared. A total of 2941 genes were differentially expressed, including 1422 genes upregulated and 1519 genes downregulated under arid conditions. Oxidative stress-responsive regulatory genes perR, fur, and tipA were significantly upregulated, along with DNA protecting protein (dps), and catalase (katE). Genes related to Fe(2+) uptake (feoB), sporulation stage II (spoIIB, spoIIE, spoIIGA), small acid-soluble spore protein (sspD), and biosynthesis of compatible solute ectoine (ectB, ectA) were also highly expressed to various degrees. Oxidative phosphorylation-related genes (atpB, atpE, atpF, atpH, atpA, atpG, atpD, atpC) and glycolysis-related genes (pgk, tpiA, frmA) were significantly downregulated. CONCLUSION: This is the first report about transcriptomic analysis of a B. megaterium to explore the mechanism of arid resistance. Major changes in transcription were seen in the arid condition simulated by PEG200 (15%), with the most important one being genes related to oxidative stress. The results showed a complex mechanism for the bacteria to adapt to arid stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-020-02039-4.
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spelling pubmed-76706812020-11-18 Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress Zhao, Lei Zhou, Yanjun Li, Jianbei Xia, Yucheng Wang, Weiyun Luo, Xiuqi Yin, Juan Zhong, Jiang BMC Microbiol Research Article BACKGROUND: For microorganisms on a paper surface, the lack of water is one of the most important stress factors. A strain of Bacillus megaterium FDU301 was isolated from plaques on a paper surface using culture medium with polyethylene glycol 200 (PEG200) to simulate an arid condition. Global transcriptomic analysis of B. megaterium FDU301 grown under normal and simulated arid conditions was performed via RNA-seq technology to identify genes involved in arid stress adaptation. RESULTS: The transcriptome of B. megaterium FDU301 grown in LB medium under arid (15% PEG200 (w/w)) and normal conditions were compared. A total of 2941 genes were differentially expressed, including 1422 genes upregulated and 1519 genes downregulated under arid conditions. Oxidative stress-responsive regulatory genes perR, fur, and tipA were significantly upregulated, along with DNA protecting protein (dps), and catalase (katE). Genes related to Fe(2+) uptake (feoB), sporulation stage II (spoIIB, spoIIE, spoIIGA), small acid-soluble spore protein (sspD), and biosynthesis of compatible solute ectoine (ectB, ectA) were also highly expressed to various degrees. Oxidative phosphorylation-related genes (atpB, atpE, atpF, atpH, atpA, atpG, atpD, atpC) and glycolysis-related genes (pgk, tpiA, frmA) were significantly downregulated. CONCLUSION: This is the first report about transcriptomic analysis of a B. megaterium to explore the mechanism of arid resistance. Major changes in transcription were seen in the arid condition simulated by PEG200 (15%), with the most important one being genes related to oxidative stress. The results showed a complex mechanism for the bacteria to adapt to arid stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-020-02039-4. BioMed Central 2020-11-16 /pmc/articles/PMC7670681/ /pubmed/33198631 http://dx.doi.org/10.1186/s12866-020-02039-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Zhao, Lei
Zhou, Yanjun
Li, Jianbei
Xia, Yucheng
Wang, Weiyun
Luo, Xiuqi
Yin, Juan
Zhong, Jiang
Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress
title Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress
title_full Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress
title_fullStr Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress
title_full_unstemmed Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress
title_short Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress
title_sort transcriptional response of bacillus megaterium fdu301 to peg200-mediated arid stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670681/
https://www.ncbi.nlm.nih.gov/pubmed/33198631
http://dx.doi.org/10.1186/s12866-020-02039-4
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