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Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato

BACKGROUND: Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant g...

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Autores principales: Steiner, Evyatar, Israeli, Alon, Gupta, Rupali, Shwartz, Ido, Nir, Ido, Leibman-Markus, Meirav, Tal, Lior, Farber, Mika, Amsalem, Ziva, Ori, Naomi, Müller, Bruno, Bar, Maya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670716/
https://www.ncbi.nlm.nih.gov/pubmed/33292327
http://dx.doi.org/10.1186/s13007-020-00694-2
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author Steiner, Evyatar
Israeli, Alon
Gupta, Rupali
Shwartz, Ido
Nir, Ido
Leibman-Markus, Meirav
Tal, Lior
Farber, Mika
Amsalem, Ziva
Ori, Naomi
Müller, Bruno
Bar, Maya
author_facet Steiner, Evyatar
Israeli, Alon
Gupta, Rupali
Shwartz, Ido
Nir, Ido
Leibman-Markus, Meirav
Tal, Lior
Farber, Mika
Amsalem, Ziva
Ori, Naomi
Müller, Bruno
Bar, Maya
author_sort Steiner, Evyatar
collection PubMed
description BACKGROUND: Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development. RESULTS: Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato. CONCLUSIONS: The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers.
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spelling pubmed-76707162020-11-18 Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato Steiner, Evyatar Israeli, Alon Gupta, Rupali Shwartz, Ido Nir, Ido Leibman-Markus, Meirav Tal, Lior Farber, Mika Amsalem, Ziva Ori, Naomi Müller, Bruno Bar, Maya Plant Methods Methodology BACKGROUND: Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development. RESULTS: Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato. CONCLUSIONS: The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers. BioMed Central 2020-11-16 /pmc/articles/PMC7670716/ /pubmed/33292327 http://dx.doi.org/10.1186/s13007-020-00694-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Methodology
Steiner, Evyatar
Israeli, Alon
Gupta, Rupali
Shwartz, Ido
Nir, Ido
Leibman-Markus, Meirav
Tal, Lior
Farber, Mika
Amsalem, Ziva
Ori, Naomi
Müller, Bruno
Bar, Maya
Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato
title Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato
title_full Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato
title_fullStr Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato
title_full_unstemmed Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato
title_short Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato
title_sort characterization of the cytokinin sensor tcsv2 in arabidopsis and tomato
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670716/
https://www.ncbi.nlm.nih.gov/pubmed/33292327
http://dx.doi.org/10.1186/s13007-020-00694-2
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