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Osmotic stress alters circadian cytosolic Ca(2+) oscillations and OSCA1 is required in circadian gated stress adaptation

The circadian clock is a universal timing system that involved in plant physical responses to abiotic stresses. Moreover, OSCA1 is an osmosensor responsible for [Ca(2+)](i) increases induced by osmotic stress in plants. However, there is little information on osmosensor involved osmotic stress-trigg...

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Autores principales: Zhang, Shu, Wu, Qian-Rong, Liu, Lu-Lu, Zhang, Hui-Min, Gao, Jian-Wei, Pei, Zhen-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671097/
https://www.ncbi.nlm.nih.gov/pubmed/33100175
http://dx.doi.org/10.1080/15592324.2020.1836883
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author Zhang, Shu
Wu, Qian-Rong
Liu, Lu-Lu
Zhang, Hui-Min
Gao, Jian-Wei
Pei, Zhen-Ming
author_facet Zhang, Shu
Wu, Qian-Rong
Liu, Lu-Lu
Zhang, Hui-Min
Gao, Jian-Wei
Pei, Zhen-Ming
author_sort Zhang, Shu
collection PubMed
description The circadian clock is a universal timing system that involved in plant physical responses to abiotic stresses. Moreover, OSCA1 is an osmosensor responsible for [Ca(2+)](i) increases induced by osmotic stress in plants. However, there is little information on osmosensor involved osmotic stress-triggered circadian clock responses. Using an aequorin-based Ca(2+) imaging assay, we found the gradient (0 mM, 200 mM, 500 mM) osmotic stress (induced by sorbitol) both altered the primary circadian parameter of WT and osca1 mutant. This means the plant switch to a fast day/night model to avoid energy consumption. In contrast, the period of WT and osca1 mutant became short since the sorbitol concentration increased from 0 mM to 500 mM. As the sorbitol concentration increased, the phase of the WT becomes more extensive compared with osca1 mutant, which means WT is more capable of coping with the environmental change. Moreover, the amplitude of WT also becomes broader than osca1 mutant, especially in high (500 mM) sorbitol concentration, indicate the WT shows more responses in high osmotic stress. In a word, the WT has much more flexibility to cope with the osmotic stress than osca1 mutant. It implies the OSCA1 might be involved in the circadian gated plant adaptation to the environmental osmotic stress, which opens an avenue to study Ca(2+) processes with other circadian signaling pathways.
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spelling pubmed-76710972020-11-23 Osmotic stress alters circadian cytosolic Ca(2+) oscillations and OSCA1 is required in circadian gated stress adaptation Zhang, Shu Wu, Qian-Rong Liu, Lu-Lu Zhang, Hui-Min Gao, Jian-Wei Pei, Zhen-Ming Plant Signal Behav Research Paper The circadian clock is a universal timing system that involved in plant physical responses to abiotic stresses. Moreover, OSCA1 is an osmosensor responsible for [Ca(2+)](i) increases induced by osmotic stress in plants. However, there is little information on osmosensor involved osmotic stress-triggered circadian clock responses. Using an aequorin-based Ca(2+) imaging assay, we found the gradient (0 mM, 200 mM, 500 mM) osmotic stress (induced by sorbitol) both altered the primary circadian parameter of WT and osca1 mutant. This means the plant switch to a fast day/night model to avoid energy consumption. In contrast, the period of WT and osca1 mutant became short since the sorbitol concentration increased from 0 mM to 500 mM. As the sorbitol concentration increased, the phase of the WT becomes more extensive compared with osca1 mutant, which means WT is more capable of coping with the environmental change. Moreover, the amplitude of WT also becomes broader than osca1 mutant, especially in high (500 mM) sorbitol concentration, indicate the WT shows more responses in high osmotic stress. In a word, the WT has much more flexibility to cope with the osmotic stress than osca1 mutant. It implies the OSCA1 might be involved in the circadian gated plant adaptation to the environmental osmotic stress, which opens an avenue to study Ca(2+) processes with other circadian signaling pathways. Taylor & Francis 2020-10-24 /pmc/articles/PMC7671097/ /pubmed/33100175 http://dx.doi.org/10.1080/15592324.2020.1836883 Text en © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Research Paper
Zhang, Shu
Wu, Qian-Rong
Liu, Lu-Lu
Zhang, Hui-Min
Gao, Jian-Wei
Pei, Zhen-Ming
Osmotic stress alters circadian cytosolic Ca(2+) oscillations and OSCA1 is required in circadian gated stress adaptation
title Osmotic stress alters circadian cytosolic Ca(2+) oscillations and OSCA1 is required in circadian gated stress adaptation
title_full Osmotic stress alters circadian cytosolic Ca(2+) oscillations and OSCA1 is required in circadian gated stress adaptation
title_fullStr Osmotic stress alters circadian cytosolic Ca(2+) oscillations and OSCA1 is required in circadian gated stress adaptation
title_full_unstemmed Osmotic stress alters circadian cytosolic Ca(2+) oscillations and OSCA1 is required in circadian gated stress adaptation
title_short Osmotic stress alters circadian cytosolic Ca(2+) oscillations and OSCA1 is required in circadian gated stress adaptation
title_sort osmotic stress alters circadian cytosolic ca(2+) oscillations and osca1 is required in circadian gated stress adaptation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671097/
https://www.ncbi.nlm.nih.gov/pubmed/33100175
http://dx.doi.org/10.1080/15592324.2020.1836883
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