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Identification of gene fusion events in Mycobacterium tuberculosis that encode chimeric proteins

Mycobacterium tuberculosis is a facultative intracellular pathogen responsible for causing tuberculosis. The harsh environment in which M. tuberculosis survives requires this pathogen to continuously adapt in order to maintain an evolutionary advantage. However, the apparent absence of horizontal ge...

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Autores principales: Gallant, James, Mouton, Jomien, Ummels, Roy, ten Hagen-Jongman, Corinne, Kriel, Nastassja, Pain, Arnab, Warren, Robin M, Bitter, Wilbert, Heunis, Tiaan, Sampson, Samantha L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671302/
https://www.ncbi.nlm.nih.gov/pubmed/33575588
http://dx.doi.org/10.1093/nargab/lqaa033
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author Gallant, James
Mouton, Jomien
Ummels, Roy
ten Hagen-Jongman, Corinne
Kriel, Nastassja
Pain, Arnab
Warren, Robin M
Bitter, Wilbert
Heunis, Tiaan
Sampson, Samantha L
author_facet Gallant, James
Mouton, Jomien
Ummels, Roy
ten Hagen-Jongman, Corinne
Kriel, Nastassja
Pain, Arnab
Warren, Robin M
Bitter, Wilbert
Heunis, Tiaan
Sampson, Samantha L
author_sort Gallant, James
collection PubMed
description Mycobacterium tuberculosis is a facultative intracellular pathogen responsible for causing tuberculosis. The harsh environment in which M. tuberculosis survives requires this pathogen to continuously adapt in order to maintain an evolutionary advantage. However, the apparent absence of horizontal gene transfer in M. tuberculosis imposes restrictions in the ways by which evolution can occur. Large-scale changes in the genome can be introduced through genome reduction, recombination events and structural variation. Here, we identify a functional chimeric protein in the ppe38–71 locus, the absence of which is known to have an impact on protein secretion and virulence. To examine whether this approach was used more often by this pathogen, we further develop software that detects potential gene fusion events from multigene deletions using whole genome sequencing data. With this software we could identify a number of other putative gene fusion events within the genomes of M. tuberculosis isolates. We were able to demonstrate the expression of one of these gene fusions at the protein level using mass spectrometry. Therefore, gene fusions may provide an additional means of evolution for M. tuberculosis in its natural environment whereby novel chimeric proteins and functions can arise.
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spelling pubmed-76713022021-02-10 Identification of gene fusion events in Mycobacterium tuberculosis that encode chimeric proteins Gallant, James Mouton, Jomien Ummels, Roy ten Hagen-Jongman, Corinne Kriel, Nastassja Pain, Arnab Warren, Robin M Bitter, Wilbert Heunis, Tiaan Sampson, Samantha L NAR Genom Bioinform Standard Article Mycobacterium tuberculosis is a facultative intracellular pathogen responsible for causing tuberculosis. The harsh environment in which M. tuberculosis survives requires this pathogen to continuously adapt in order to maintain an evolutionary advantage. However, the apparent absence of horizontal gene transfer in M. tuberculosis imposes restrictions in the ways by which evolution can occur. Large-scale changes in the genome can be introduced through genome reduction, recombination events and structural variation. Here, we identify a functional chimeric protein in the ppe38–71 locus, the absence of which is known to have an impact on protein secretion and virulence. To examine whether this approach was used more often by this pathogen, we further develop software that detects potential gene fusion events from multigene deletions using whole genome sequencing data. With this software we could identify a number of other putative gene fusion events within the genomes of M. tuberculosis isolates. We were able to demonstrate the expression of one of these gene fusions at the protein level using mass spectrometry. Therefore, gene fusions may provide an additional means of evolution for M. tuberculosis in its natural environment whereby novel chimeric proteins and functions can arise. Oxford University Press 2020-05-18 /pmc/articles/PMC7671302/ /pubmed/33575588 http://dx.doi.org/10.1093/nargab/lqaa033 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of NAR Genomics and Bioinformatics. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Standard Article
Gallant, James
Mouton, Jomien
Ummels, Roy
ten Hagen-Jongman, Corinne
Kriel, Nastassja
Pain, Arnab
Warren, Robin M
Bitter, Wilbert
Heunis, Tiaan
Sampson, Samantha L
Identification of gene fusion events in Mycobacterium tuberculosis that encode chimeric proteins
title Identification of gene fusion events in Mycobacterium tuberculosis that encode chimeric proteins
title_full Identification of gene fusion events in Mycobacterium tuberculosis that encode chimeric proteins
title_fullStr Identification of gene fusion events in Mycobacterium tuberculosis that encode chimeric proteins
title_full_unstemmed Identification of gene fusion events in Mycobacterium tuberculosis that encode chimeric proteins
title_short Identification of gene fusion events in Mycobacterium tuberculosis that encode chimeric proteins
title_sort identification of gene fusion events in mycobacterium tuberculosis that encode chimeric proteins
topic Standard Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671302/
https://www.ncbi.nlm.nih.gov/pubmed/33575588
http://dx.doi.org/10.1093/nargab/lqaa033
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