Nanopore sequencing of native adeno-associated virus (AAV) single-stranded DNA using a transposase-based rapid protocol

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Next-generation sequencing of single-stranded DNA (ssDNA) enables transgene characterization of gene therapy vectors such as adeno-associated virus (AAV), but current library generation uses complicated and potentially biased second-strand synthesis. We report that libraries for nanopore sequencing...

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Detalles Bibliográficos
Autores principales: Radukic, Marco T, Brandt, David, Haak, Markus, Müller, Kristian M, Kalinowski, Jörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Standard Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671332/
https://www.ncbi.nlm.nih.gov/pubmed/33575623
http://dx.doi.org/10.1093/nargab/lqaa074

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