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Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals

Implementation of lateral flow devices (LFDs) for rabies antigen detection is expected to improve surveillance through the efficient detection of rabid animals in resource-limited settings; however, the use of LFDs for diagnosis remains controversial because some commercially available kits show low...

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Autores principales: Kimitsuki, Kazunori, Saito, Nobuo, Yamada, Kentaro, Park, Chun-Ho, Inoue, Satoshi, Suzuki, Motoi, Saito-Obata, Mariko, Kamiya, Yasuhiko, Manalo, Daria L., Demetria, Catalino S., Mananggit, Milagros R., Quiambao, Beatriz P., Nishizono, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671516/
https://www.ncbi.nlm.nih.gov/pubmed/33151941
http://dx.doi.org/10.1371/journal.pntd.0008844
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author Kimitsuki, Kazunori
Saito, Nobuo
Yamada, Kentaro
Park, Chun-Ho
Inoue, Satoshi
Suzuki, Motoi
Saito-Obata, Mariko
Kamiya, Yasuhiko
Manalo, Daria L.
Demetria, Catalino S.
Mananggit, Milagros R.
Quiambao, Beatriz P.
Nishizono, Akira
author_facet Kimitsuki, Kazunori
Saito, Nobuo
Yamada, Kentaro
Park, Chun-Ho
Inoue, Satoshi
Suzuki, Motoi
Saito-Obata, Mariko
Kamiya, Yasuhiko
Manalo, Daria L.
Demetria, Catalino S.
Mananggit, Milagros R.
Quiambao, Beatriz P.
Nishizono, Akira
author_sort Kimitsuki, Kazunori
collection PubMed
description Implementation of lateral flow devices (LFDs) for rabies antigen detection is expected to improve surveillance through the efficient detection of rabid animals in resource-limited settings; however, the use of LFDs for diagnosis remains controversial because some commercially available kits show low sensitivity. Therefore, we compared the diagnostic efficacy of three LFDs (ADTEC, Bionote, and Elabscience kits) paralleled with the direct fluorescent antibody test (dFAT) using fresh samples and investigated the diagnostic accuracies. To do so, we evaluated rabies-suspected samples submitted to the Regional Animal Disease Diagnostic Laboratory III, Philippines. Furthermore, we conducted real-time RT-PCR and sequencing to measure the accuracy of field laboratory diagnosis. The total number of animals submitted during this study period was 184 cases, including negative control samples. Of these, 53.9% (84 cases) were positive in the dFAT. Dogs were the most common rabies-suspected animal (n = 135). The sensitivities of the ADTEC and Bionote kits were 0.88 (74 cases) and 0.95 (80 cases), respectively. The specificity of both kits was 1.00 (100 cases). Furthermore, the sensitivity and specificity of the ADTEC kit after directly homogenizing the samples in assay buffer without dilution in phosphate-buffered saline (ADTEC kit DM) were 0.94 (79 cases) and 1.00 (100 cases), respectively. By contrast, there were no positive results using the Elabscience kit among all dFAT-positive samples. The sensitivity and specificity of LFDs make these tests highly feasible if properly used. Therefore, LFD tests can be used to strengthen the surveillance of rabies-infected animals in endemic and resource-limited settings.
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spelling pubmed-76715162020-11-19 Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals Kimitsuki, Kazunori Saito, Nobuo Yamada, Kentaro Park, Chun-Ho Inoue, Satoshi Suzuki, Motoi Saito-Obata, Mariko Kamiya, Yasuhiko Manalo, Daria L. Demetria, Catalino S. Mananggit, Milagros R. Quiambao, Beatriz P. Nishizono, Akira PLoS Negl Trop Dis Research Article Implementation of lateral flow devices (LFDs) for rabies antigen detection is expected to improve surveillance through the efficient detection of rabid animals in resource-limited settings; however, the use of LFDs for diagnosis remains controversial because some commercially available kits show low sensitivity. Therefore, we compared the diagnostic efficacy of three LFDs (ADTEC, Bionote, and Elabscience kits) paralleled with the direct fluorescent antibody test (dFAT) using fresh samples and investigated the diagnostic accuracies. To do so, we evaluated rabies-suspected samples submitted to the Regional Animal Disease Diagnostic Laboratory III, Philippines. Furthermore, we conducted real-time RT-PCR and sequencing to measure the accuracy of field laboratory diagnosis. The total number of animals submitted during this study period was 184 cases, including negative control samples. Of these, 53.9% (84 cases) were positive in the dFAT. Dogs were the most common rabies-suspected animal (n = 135). The sensitivities of the ADTEC and Bionote kits were 0.88 (74 cases) and 0.95 (80 cases), respectively. The specificity of both kits was 1.00 (100 cases). Furthermore, the sensitivity and specificity of the ADTEC kit after directly homogenizing the samples in assay buffer without dilution in phosphate-buffered saline (ADTEC kit DM) were 0.94 (79 cases) and 1.00 (100 cases), respectively. By contrast, there were no positive results using the Elabscience kit among all dFAT-positive samples. The sensitivity and specificity of LFDs make these tests highly feasible if properly used. Therefore, LFD tests can be used to strengthen the surveillance of rabies-infected animals in endemic and resource-limited settings. Public Library of Science 2020-11-05 /pmc/articles/PMC7671516/ /pubmed/33151941 http://dx.doi.org/10.1371/journal.pntd.0008844 Text en © 2020 Kimitsuki et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kimitsuki, Kazunori
Saito, Nobuo
Yamada, Kentaro
Park, Chun-Ho
Inoue, Satoshi
Suzuki, Motoi
Saito-Obata, Mariko
Kamiya, Yasuhiko
Manalo, Daria L.
Demetria, Catalino S.
Mananggit, Milagros R.
Quiambao, Beatriz P.
Nishizono, Akira
Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals
title Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals
title_full Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals
title_fullStr Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals
title_full_unstemmed Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals
title_short Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals
title_sort evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671516/
https://www.ncbi.nlm.nih.gov/pubmed/33151941
http://dx.doi.org/10.1371/journal.pntd.0008844
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