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Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose

Enterococcus mundtii QU25, a non-dairy lactic acid bacterium of the phylum Firmicutes, is capable of simultaneously fermenting cellobiose and xylose, and is described as a promising strain for the industrial production of optically pure l-lactic acid (≥ 99.9%) via homo-fermentation of lignocellulosi...

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Autores principales: Shiwa, Yuh, Fujiwara, Haruko, Numaguchi, Mao, Abdel-Rahman, Mohamed Ali, Nabeta, Keisuke, Kanesaki, Yu, Tashiro, Yukihiro, Zendo, Takeshi, Tanaka, Naoto, Fujita, Nobuyuki, Yoshikawa, Hirofumi, Sonomoto, Kenji, Shimizu-Kadota, Mariko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671544/
https://www.ncbi.nlm.nih.gov/pubmed/33201910
http://dx.doi.org/10.1371/journal.pone.0242070
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author Shiwa, Yuh
Fujiwara, Haruko
Numaguchi, Mao
Abdel-Rahman, Mohamed Ali
Nabeta, Keisuke
Kanesaki, Yu
Tashiro, Yukihiro
Zendo, Takeshi
Tanaka, Naoto
Fujita, Nobuyuki
Yoshikawa, Hirofumi
Sonomoto, Kenji
Shimizu-Kadota, Mariko
author_facet Shiwa, Yuh
Fujiwara, Haruko
Numaguchi, Mao
Abdel-Rahman, Mohamed Ali
Nabeta, Keisuke
Kanesaki, Yu
Tashiro, Yukihiro
Zendo, Takeshi
Tanaka, Naoto
Fujita, Nobuyuki
Yoshikawa, Hirofumi
Sonomoto, Kenji
Shimizu-Kadota, Mariko
author_sort Shiwa, Yuh
collection PubMed
description Enterococcus mundtii QU25, a non-dairy lactic acid bacterium of the phylum Firmicutes, is capable of simultaneously fermenting cellobiose and xylose, and is described as a promising strain for the industrial production of optically pure l-lactic acid (≥ 99.9%) via homo-fermentation of lignocellulosic hydrolysates. Generally, Firmicutes bacteria show preferential consumption of sugar (usually glucose), termed carbon catabolite repression (CCR), while hampering the catabolism of other sugars. In our previous study, QU25 exhibited apparent CCR in a glucose-xylose mixture phenotypically, and transcriptional repression of the xylose operon encoding initial xylose metabolism genes, likely occurred in a CcpA-dependent manner. QU25 did not exhibit CCR phenotypically in a cellobiose-xylose mixture. The aim of the current study is to elucidate the transcriptional change associated with the simultaneous utilization of cellobiose and xylose. To this end, we performed RNA-seq analysis in the exponential growth phase of E. mundtii QU25 cells grown in glucose, cellobiose, and/or xylose as either sole or co-carbon sources. Our transcriptomic data showed that the xylose operon was weakly repressed in cells grown in a cellobiose-xylose mixture compared with that in cells grown in a glucose-xylose mixture. Furthermore, the gene expression of talC, the sole gene encoding transaldolase, is expected to be repressed by CcpA-mediated CCR. QU25 metabolized xylose without using transaldolase, which is necessary for homolactic fermentation from pentoses using the pentose-phosphate pathway. Hence, the metabolism of xylose in the presence of cellobiose by QU25 may have been due to 1) sufficient amounts of proteins encoded by the xylose operon genes for xylose metabolism despite of the slight repression of the operon, and 2) bypassing of the pentose-phosphate pathway without the TalC activity. Accordingly, we have determined the targets of genetic modification in QU25 to metabolize cellobiose, xylose and glucose simultaneously for application of the lactic fermentation from lignocellulosic hydrolysates.
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spelling pubmed-76715442020-11-19 Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose Shiwa, Yuh Fujiwara, Haruko Numaguchi, Mao Abdel-Rahman, Mohamed Ali Nabeta, Keisuke Kanesaki, Yu Tashiro, Yukihiro Zendo, Takeshi Tanaka, Naoto Fujita, Nobuyuki Yoshikawa, Hirofumi Sonomoto, Kenji Shimizu-Kadota, Mariko PLoS One Research Article Enterococcus mundtii QU25, a non-dairy lactic acid bacterium of the phylum Firmicutes, is capable of simultaneously fermenting cellobiose and xylose, and is described as a promising strain for the industrial production of optically pure l-lactic acid (≥ 99.9%) via homo-fermentation of lignocellulosic hydrolysates. Generally, Firmicutes bacteria show preferential consumption of sugar (usually glucose), termed carbon catabolite repression (CCR), while hampering the catabolism of other sugars. In our previous study, QU25 exhibited apparent CCR in a glucose-xylose mixture phenotypically, and transcriptional repression of the xylose operon encoding initial xylose metabolism genes, likely occurred in a CcpA-dependent manner. QU25 did not exhibit CCR phenotypically in a cellobiose-xylose mixture. The aim of the current study is to elucidate the transcriptional change associated with the simultaneous utilization of cellobiose and xylose. To this end, we performed RNA-seq analysis in the exponential growth phase of E. mundtii QU25 cells grown in glucose, cellobiose, and/or xylose as either sole or co-carbon sources. Our transcriptomic data showed that the xylose operon was weakly repressed in cells grown in a cellobiose-xylose mixture compared with that in cells grown in a glucose-xylose mixture. Furthermore, the gene expression of talC, the sole gene encoding transaldolase, is expected to be repressed by CcpA-mediated CCR. QU25 metabolized xylose without using transaldolase, which is necessary for homolactic fermentation from pentoses using the pentose-phosphate pathway. Hence, the metabolism of xylose in the presence of cellobiose by QU25 may have been due to 1) sufficient amounts of proteins encoded by the xylose operon genes for xylose metabolism despite of the slight repression of the operon, and 2) bypassing of the pentose-phosphate pathway without the TalC activity. Accordingly, we have determined the targets of genetic modification in QU25 to metabolize cellobiose, xylose and glucose simultaneously for application of the lactic fermentation from lignocellulosic hydrolysates. Public Library of Science 2020-11-17 /pmc/articles/PMC7671544/ /pubmed/33201910 http://dx.doi.org/10.1371/journal.pone.0242070 Text en © 2020 Shiwa et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shiwa, Yuh
Fujiwara, Haruko
Numaguchi, Mao
Abdel-Rahman, Mohamed Ali
Nabeta, Keisuke
Kanesaki, Yu
Tashiro, Yukihiro
Zendo, Takeshi
Tanaka, Naoto
Fujita, Nobuyuki
Yoshikawa, Hirofumi
Sonomoto, Kenji
Shimizu-Kadota, Mariko
Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose
title Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose
title_full Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose
title_fullStr Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose
title_full_unstemmed Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose
title_short Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose
title_sort transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium enterococcus mundtii qu25 grown in media with combinations of cellobiose, xylose, and glucose
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671544/
https://www.ncbi.nlm.nih.gov/pubmed/33201910
http://dx.doi.org/10.1371/journal.pone.0242070
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