Growth differentiation factor 6, a repressive target of EZH2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells

Mesenchymal stem cells (MSCs) derived from human embryonic stem cells (hESCs) have significant potential for cell-mediated bone regeneration. Our recent study revealed that inhibiting the epigenetic regulator EZH2 plays a key role in promoting the mesodermal differentiation of hESCs. In this study,...

Descripción completa

Detalles Bibliográficos
Autores principales: Deng, Pend, Yu, Yongxin, Hong, Christine, Wang, Cun-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672114/
https://www.ncbi.nlm.nih.gov/pubmed/33298857
http://dx.doi.org/10.1038/s41413-020-00116-y
_version_ 1783611063133011968
author Deng, Pend
Yu, Yongxin
Hong, Christine
Wang, Cun-Yu
author_facet Deng, Pend
Yu, Yongxin
Hong, Christine
Wang, Cun-Yu
author_sort Deng, Pend
collection PubMed
description Mesenchymal stem cells (MSCs) derived from human embryonic stem cells (hESCs) have significant potential for cell-mediated bone regeneration. Our recent study revealed that inhibiting the epigenetic regulator EZH2 plays a key role in promoting the mesodermal differentiation of hESCs. In this study, an epigenome-wide analysis of hESCs and MSCs revealed that growth differentiation factor 6 (GDF6), which is involved in bone formation, was the most upregulated gene associated with MSCs compared to hESCs. Furthermore, we identified GDF6 as a repressive target of EZH2 and found that ectopic GDF6 selectively promoted hESC differentiation towards the mesodermal lineage and enriched the MSC population. Our results provide molecular insights governing the mesenchymal commitment of hESCs and identify an inducing factor that offers strong promise for the future of regenerative medicine.
format Online
Article
Text
id pubmed-7672114
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-76721142020-11-20 Growth differentiation factor 6, a repressive target of EZH2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells Deng, Pend Yu, Yongxin Hong, Christine Wang, Cun-Yu Bone Res Article Mesenchymal stem cells (MSCs) derived from human embryonic stem cells (hESCs) have significant potential for cell-mediated bone regeneration. Our recent study revealed that inhibiting the epigenetic regulator EZH2 plays a key role in promoting the mesodermal differentiation of hESCs. In this study, an epigenome-wide analysis of hESCs and MSCs revealed that growth differentiation factor 6 (GDF6), which is involved in bone formation, was the most upregulated gene associated with MSCs compared to hESCs. Furthermore, we identified GDF6 as a repressive target of EZH2 and found that ectopic GDF6 selectively promoted hESC differentiation towards the mesodermal lineage and enriched the MSC population. Our results provide molecular insights governing the mesenchymal commitment of hESCs and identify an inducing factor that offers strong promise for the future of regenerative medicine. Nature Publishing Group UK 2020-11-17 /pmc/articles/PMC7672114/ /pubmed/33298857 http://dx.doi.org/10.1038/s41413-020-00116-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Deng, Pend
Yu, Yongxin
Hong, Christine
Wang, Cun-Yu
Growth differentiation factor 6, a repressive target of EZH2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells
title Growth differentiation factor 6, a repressive target of EZH2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells
title_full Growth differentiation factor 6, a repressive target of EZH2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells
title_fullStr Growth differentiation factor 6, a repressive target of EZH2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells
title_full_unstemmed Growth differentiation factor 6, a repressive target of EZH2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells
title_short Growth differentiation factor 6, a repressive target of EZH2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells
title_sort growth differentiation factor 6, a repressive target of ezh2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672114/
https://www.ncbi.nlm.nih.gov/pubmed/33298857
http://dx.doi.org/10.1038/s41413-020-00116-y
work_keys_str_mv AT dengpend growthdifferentiationfactor6arepressivetargetofezh2promotesthecommitmentofhumanembryonicstemcellstomesenchymalstemcells
AT yuyongxin growthdifferentiationfactor6arepressivetargetofezh2promotesthecommitmentofhumanembryonicstemcellstomesenchymalstemcells
AT hongchristine growthdifferentiationfactor6arepressivetargetofezh2promotesthecommitmentofhumanembryonicstemcellstomesenchymalstemcells
AT wangcunyu growthdifferentiationfactor6arepressivetargetofezh2promotesthecommitmentofhumanembryonicstemcellstomesenchymalstemcells

Ejemplares similares