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Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells

Meningioma (MEN) is a common central nervous system disease. Accumulating evidence indicated that long non-coding RNA maternally expressed gene 3 (MEG3) participated in the progression of MEN. However, the potential mechanisms of MEG3 in altering the aggressive phenotypes of MEN need further explora...

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Autores principales: Ding, Chenyu, Yi, Xuehan, Xu, Jiaheng, Huang, Zhenhua, Bu, Xingyao, Wang, Desheng, Ge, Hongliang, Zhang, Gaoqi, Gu, Jianjun, Kang, Dezhi, Wu, Xiyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672212/
https://www.ncbi.nlm.nih.gov/pubmed/33251130
http://dx.doi.org/10.3389/fonc.2020.537763
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author Ding, Chenyu
Yi, Xuehan
Xu, Jiaheng
Huang, Zhenhua
Bu, Xingyao
Wang, Desheng
Ge, Hongliang
Zhang, Gaoqi
Gu, Jianjun
Kang, Dezhi
Wu, Xiyue
author_facet Ding, Chenyu
Yi, Xuehan
Xu, Jiaheng
Huang, Zhenhua
Bu, Xingyao
Wang, Desheng
Ge, Hongliang
Zhang, Gaoqi
Gu, Jianjun
Kang, Dezhi
Wu, Xiyue
author_sort Ding, Chenyu
collection PubMed
description Meningioma (MEN) is a common central nervous system disease. Accumulating evidence indicated that long non-coding RNA maternally expressed gene 3 (MEG3) participated in the progression of MEN. However, the potential mechanisms of MEG3 in altering the aggressive phenotypes of MEN need further exploration. Levels of MEG3, microRNA (miR)-29c, and A-kinase anchor protein 12 (AKAP12) were determined using quantitative real-time Polymerase Chain Reaction (qRT-PCR) assay. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were performed to verify the relationship between miR-29c and MEG3 or AKAP12. The protein level of AKAP12 was detected by western blot. Moreover, cell-cycle arrest, migration, invasion, and proliferation were assessed by flow cytometry, wound healing, transwell assays, and CCK-8 assay, respectively. Levels of MEG3 and AKAP12 were downregulated, while miR-29c was effectively increased in MEN tissues and cell line. Mechanically, MEG3 was a sponge of miR-29c to regulate the expression of AKAP12. Functionally, increase of MEG3 diminished cell-cycle, migration, invasion, and proliferation in MEN cells, and reintroduction of miR-29c could eliminate these effects. In addition, AKAP12 depletion overturned the inhibitory effects of miR-29c absence on cell-cycle, migration, invasion, and proliferation in vitro. Also, AKAP12 was co-regulated by MEG3/miR-29c axis. MEG3 mediated the aggressive behaviors of MEN cells via miR-29c/AKAP12 axis, supporting that MEG3 served as a promising biomarker for the diagnosis and treatment of human MEN.
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spelling pubmed-76722122020-11-26 Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells Ding, Chenyu Yi, Xuehan Xu, Jiaheng Huang, Zhenhua Bu, Xingyao Wang, Desheng Ge, Hongliang Zhang, Gaoqi Gu, Jianjun Kang, Dezhi Wu, Xiyue Front Oncol Oncology Meningioma (MEN) is a common central nervous system disease. Accumulating evidence indicated that long non-coding RNA maternally expressed gene 3 (MEG3) participated in the progression of MEN. However, the potential mechanisms of MEG3 in altering the aggressive phenotypes of MEN need further exploration. Levels of MEG3, microRNA (miR)-29c, and A-kinase anchor protein 12 (AKAP12) were determined using quantitative real-time Polymerase Chain Reaction (qRT-PCR) assay. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were performed to verify the relationship between miR-29c and MEG3 or AKAP12. The protein level of AKAP12 was detected by western blot. Moreover, cell-cycle arrest, migration, invasion, and proliferation were assessed by flow cytometry, wound healing, transwell assays, and CCK-8 assay, respectively. Levels of MEG3 and AKAP12 were downregulated, while miR-29c was effectively increased in MEN tissues and cell line. Mechanically, MEG3 was a sponge of miR-29c to regulate the expression of AKAP12. Functionally, increase of MEG3 diminished cell-cycle, migration, invasion, and proliferation in MEN cells, and reintroduction of miR-29c could eliminate these effects. In addition, AKAP12 depletion overturned the inhibitory effects of miR-29c absence on cell-cycle, migration, invasion, and proliferation in vitro. Also, AKAP12 was co-regulated by MEG3/miR-29c axis. MEG3 mediated the aggressive behaviors of MEN cells via miR-29c/AKAP12 axis, supporting that MEG3 served as a promising biomarker for the diagnosis and treatment of human MEN. Frontiers Media S.A. 2020-11-04 /pmc/articles/PMC7672212/ /pubmed/33251130 http://dx.doi.org/10.3389/fonc.2020.537763 Text en Copyright © 2020 Ding, Yi, Xu, Huang, Bu, Wang, Ge, Zhang, Gu, Kang and Wu http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Ding, Chenyu
Yi, Xuehan
Xu, Jiaheng
Huang, Zhenhua
Bu, Xingyao
Wang, Desheng
Ge, Hongliang
Zhang, Gaoqi
Gu, Jianjun
Kang, Dezhi
Wu, Xiyue
Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells
title Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells
title_full Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells
title_fullStr Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells
title_full_unstemmed Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells
title_short Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells
title_sort long non-coding rna meg3 modifies cell-cycle, migration, invasion, and proliferation through akap12 by sponging mir-29c in meningioma cells
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672212/
https://www.ncbi.nlm.nih.gov/pubmed/33251130
http://dx.doi.org/10.3389/fonc.2020.537763
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