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Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation
Phosphate (Pi) is a pivotal nutrient that constraints plant development and productivity in natural ecosystems. Land colonization by plants, more than 470 million years ago, evolved adaptive mechanisms to conquer Pi-scarce environments. However, little is known about the molecular basis underlying s...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672586/ https://www.ncbi.nlm.nih.gov/pubmed/33171770 http://dx.doi.org/10.3390/ijms21218354 |
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author | Rico-Reséndiz, Félix Cervantes-Pérez, Sergio Alan Espinal-Centeno, Annie Dipp-Álvarez, Melissa Oropeza-Aburto, Araceli Hurtado-Bautista, Enrique Cruz-Hernández, Andrés Bowman, John L. Ishizaki, Kimitsune Arteaga-Vázquez, Mario A. Herrera-Estrella, Luis Cruz-Ramírez, Alfredo |
author_facet | Rico-Reséndiz, Félix Cervantes-Pérez, Sergio Alan Espinal-Centeno, Annie Dipp-Álvarez, Melissa Oropeza-Aburto, Araceli Hurtado-Bautista, Enrique Cruz-Hernández, Andrés Bowman, John L. Ishizaki, Kimitsune Arteaga-Vázquez, Mario A. Herrera-Estrella, Luis Cruz-Ramírez, Alfredo |
author_sort | Rico-Reséndiz, Félix |
collection | PubMed |
description | Phosphate (Pi) is a pivotal nutrient that constraints plant development and productivity in natural ecosystems. Land colonization by plants, more than 470 million years ago, evolved adaptive mechanisms to conquer Pi-scarce environments. However, little is known about the molecular basis underlying such adaptations at early branches of plant phylogeny. To shed light on how early divergent plants respond to Pi limitation, we analyzed the morpho-physiological and transcriptional dynamics of Marchantia polymorpha upon Pi starvation. Our phylogenomic analysis highlights some gene networks present since the Chlorophytes and others established in the Streptophytes (e.g., PHR1–SPX1 and STOP1–ALMT1, respectively). At the morpho-physiological level, the response is characterized by the induction of phosphatase activity, media acidification, accumulation of auronidins, reduction of internal Pi concentration, and developmental modifications of rhizoids. The transcriptional response involves the induction of MpPHR1, Pi transporters, lipid turnover enzymes, and MpMYB14, which is an essential transcription factor for auronidins biosynthesis. MpSTOP2 up-regulation correlates with expression changes in genes related to organic acid biosynthesis and transport, suggesting a preference for citrate exudation. An analysis of MpPHR1 binding sequences (P1BS) shows an enrichment of this cis regulatory element in differentially expressed genes. Our study unravels the strategies, at diverse levels of organization, exerted by M. polymorpha to cope with low Pi availability. |
format | Online Article Text |
id | pubmed-7672586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76725862020-11-19 Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation Rico-Reséndiz, Félix Cervantes-Pérez, Sergio Alan Espinal-Centeno, Annie Dipp-Álvarez, Melissa Oropeza-Aburto, Araceli Hurtado-Bautista, Enrique Cruz-Hernández, Andrés Bowman, John L. Ishizaki, Kimitsune Arteaga-Vázquez, Mario A. Herrera-Estrella, Luis Cruz-Ramírez, Alfredo Int J Mol Sci Article Phosphate (Pi) is a pivotal nutrient that constraints plant development and productivity in natural ecosystems. Land colonization by plants, more than 470 million years ago, evolved adaptive mechanisms to conquer Pi-scarce environments. However, little is known about the molecular basis underlying such adaptations at early branches of plant phylogeny. To shed light on how early divergent plants respond to Pi limitation, we analyzed the morpho-physiological and transcriptional dynamics of Marchantia polymorpha upon Pi starvation. Our phylogenomic analysis highlights some gene networks present since the Chlorophytes and others established in the Streptophytes (e.g., PHR1–SPX1 and STOP1–ALMT1, respectively). At the morpho-physiological level, the response is characterized by the induction of phosphatase activity, media acidification, accumulation of auronidins, reduction of internal Pi concentration, and developmental modifications of rhizoids. The transcriptional response involves the induction of MpPHR1, Pi transporters, lipid turnover enzymes, and MpMYB14, which is an essential transcription factor for auronidins biosynthesis. MpSTOP2 up-regulation correlates with expression changes in genes related to organic acid biosynthesis and transport, suggesting a preference for citrate exudation. An analysis of MpPHR1 binding sequences (P1BS) shows an enrichment of this cis regulatory element in differentially expressed genes. Our study unravels the strategies, at diverse levels of organization, exerted by M. polymorpha to cope with low Pi availability. MDPI 2020-11-07 /pmc/articles/PMC7672586/ /pubmed/33171770 http://dx.doi.org/10.3390/ijms21218354 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rico-Reséndiz, Félix Cervantes-Pérez, Sergio Alan Espinal-Centeno, Annie Dipp-Álvarez, Melissa Oropeza-Aburto, Araceli Hurtado-Bautista, Enrique Cruz-Hernández, Andrés Bowman, John L. Ishizaki, Kimitsune Arteaga-Vázquez, Mario A. Herrera-Estrella, Luis Cruz-Ramírez, Alfredo Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation |
title | Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation |
title_full | Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation |
title_fullStr | Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation |
title_full_unstemmed | Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation |
title_short | Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation |
title_sort | transcriptional and morpho-physiological responses of marchantia polymorpha upon phosphate starvation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672586/ https://www.ncbi.nlm.nih.gov/pubmed/33171770 http://dx.doi.org/10.3390/ijms21218354 |
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