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Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing

Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3...

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Autores principales: Komprda, Tomas, Sladek, Zbysek, Sevcikova, Zuzana, Svehlova, Veronika, Wijacki, Jan, Guran, Roman, Do, Tomas, Lackova, Zuzana, Polanska, Hana, Vrlikova, Lucie, Popelkova, Vendula, Michalek, Petr, Zitka, Ondrej, Buchtova, Marcela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672592/
https://www.ncbi.nlm.nih.gov/pubmed/33114430
http://dx.doi.org/10.3390/ijms21217911
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author Komprda, Tomas
Sladek, Zbysek
Sevcikova, Zuzana
Svehlova, Veronika
Wijacki, Jan
Guran, Roman
Do, Tomas
Lackova, Zuzana
Polanska, Hana
Vrlikova, Lucie
Popelkova, Vendula
Michalek, Petr
Zitka, Ondrej
Buchtova, Marcela
author_facet Komprda, Tomas
Sladek, Zbysek
Sevcikova, Zuzana
Svehlova, Veronika
Wijacki, Jan
Guran, Roman
Do, Tomas
Lackova, Zuzana
Polanska, Hana
Vrlikova, Lucie
Popelkova, Vendula
Michalek, Petr
Zitka, Ondrej
Buchtova, Marcela
author_sort Komprda, Tomas
collection PubMed
description Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wound healing in the rat model. Rats were fed the feed mixture with 8% palm oil (P), safflower oil (S), fish oil (F) or Schizochytrium microalga extract (Sch) and compared to the animals fed by control feed mixture (C). Dorsal full-thickness cutaneous excisions were performed after 52 days of feeding and skin was left to heal for an additional 12 days. Histopathological analysis of skin wounds was performed, including immune cells immunolabeling and the determination of hydroxyproline amount as well as gene expression analyses of molecules contributing to different steps of the healing. Matrix-assisted-laser-desorption-ionization mass-spectrometry-imaging (MALDI-MSI) was used to determine the amount of collagen α-1(III) chain fragment in healing samples. Treatment by Schizochytrium extract resulted in decrease in the total wound area, in contrast to the safflower oil group where the size of the wound was larger when comparing to control animals. Diet with Schizochytrium extract and safflower oils displayed a tendency to increase the number of new vessels. The number of MPO-positive cells was diminished following any of oil treatment in comparison to the control, but their highest amount was found in animals with a fish oil diet. On the other hand, the number of CD68-positive macrophages was increased, with the most significant enhancement in the fish oil and safflower oil group. Hydroxyproline concentration was the highest in the safflower oil group but it was also enhanced in all other analyzed treatments in comparison to the control. MALDI-MSI signal intensity of a collagen III fragment decreased in the sequence C > S > Sch > P > F treatment. In conclusion, we observed differences in tissue response during healing between dietary oils, with the activation of inflammation observed following the treatment with oil containing high eicosapentaenoic acid (EPA) level (fish oil) and enhanced healing features were induced by the diet with high content of docosahexaenoic acid (DHA, Schizochytrium extract).
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spelling pubmed-76725922020-11-19 Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing Komprda, Tomas Sladek, Zbysek Sevcikova, Zuzana Svehlova, Veronika Wijacki, Jan Guran, Roman Do, Tomas Lackova, Zuzana Polanska, Hana Vrlikova, Lucie Popelkova, Vendula Michalek, Petr Zitka, Ondrej Buchtova, Marcela Int J Mol Sci Article Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wound healing in the rat model. Rats were fed the feed mixture with 8% palm oil (P), safflower oil (S), fish oil (F) or Schizochytrium microalga extract (Sch) and compared to the animals fed by control feed mixture (C). Dorsal full-thickness cutaneous excisions were performed after 52 days of feeding and skin was left to heal for an additional 12 days. Histopathological analysis of skin wounds was performed, including immune cells immunolabeling and the determination of hydroxyproline amount as well as gene expression analyses of molecules contributing to different steps of the healing. Matrix-assisted-laser-desorption-ionization mass-spectrometry-imaging (MALDI-MSI) was used to determine the amount of collagen α-1(III) chain fragment in healing samples. Treatment by Schizochytrium extract resulted in decrease in the total wound area, in contrast to the safflower oil group where the size of the wound was larger when comparing to control animals. Diet with Schizochytrium extract and safflower oils displayed a tendency to increase the number of new vessels. The number of MPO-positive cells was diminished following any of oil treatment in comparison to the control, but their highest amount was found in animals with a fish oil diet. On the other hand, the number of CD68-positive macrophages was increased, with the most significant enhancement in the fish oil and safflower oil group. Hydroxyproline concentration was the highest in the safflower oil group but it was also enhanced in all other analyzed treatments in comparison to the control. MALDI-MSI signal intensity of a collagen III fragment decreased in the sequence C > S > Sch > P > F treatment. In conclusion, we observed differences in tissue response during healing between dietary oils, with the activation of inflammation observed following the treatment with oil containing high eicosapentaenoic acid (EPA) level (fish oil) and enhanced healing features were induced by the diet with high content of docosahexaenoic acid (DHA, Schizochytrium extract). MDPI 2020-10-24 /pmc/articles/PMC7672592/ /pubmed/33114430 http://dx.doi.org/10.3390/ijms21217911 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Komprda, Tomas
Sladek, Zbysek
Sevcikova, Zuzana
Svehlova, Veronika
Wijacki, Jan
Guran, Roman
Do, Tomas
Lackova, Zuzana
Polanska, Hana
Vrlikova, Lucie
Popelkova, Vendula
Michalek, Petr
Zitka, Ondrej
Buchtova, Marcela
Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing
title Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing
title_full Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing
title_fullStr Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing
title_full_unstemmed Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing
title_short Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing
title_sort comparison of dietary oils with different polyunsaturated fatty acid n-3 and n-6 content in the rat model of cutaneous wound healing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672592/
https://www.ncbi.nlm.nih.gov/pubmed/33114430
http://dx.doi.org/10.3390/ijms21217911
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