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A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome

Rejection-associated gene expression has been characterized in renal allograft biopsies for cause. The aim is to evaluate rejection gene expression in subclinical rejection and in biopsies with borderline changes or interstitial fibrosis and tubular atrophy (IFTA). We included 96 biopsies. Most diff...

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Autores principales: Chamoun, Betty, Caraben, Anna, Torres, Irina B., Sellares, Joana, Jiménez, Raquel, Toapanta, Néstor, Cidraque, Ignacio, Gabaldon, Alejandra, Perelló, Manel, Gonzalo, Ricardo, O’Valle, Francisco, Moreso, Francesc, Serón, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672640/
https://www.ncbi.nlm.nih.gov/pubmed/33153205
http://dx.doi.org/10.3390/ijms21218237
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author Chamoun, Betty
Caraben, Anna
Torres, Irina B.
Sellares, Joana
Jiménez, Raquel
Toapanta, Néstor
Cidraque, Ignacio
Gabaldon, Alejandra
Perelló, Manel
Gonzalo, Ricardo
O’Valle, Francisco
Moreso, Francesc
Serón, Daniel
author_facet Chamoun, Betty
Caraben, Anna
Torres, Irina B.
Sellares, Joana
Jiménez, Raquel
Toapanta, Néstor
Cidraque, Ignacio
Gabaldon, Alejandra
Perelló, Manel
Gonzalo, Ricardo
O’Valle, Francisco
Moreso, Francesc
Serón, Daniel
author_sort Chamoun, Betty
collection PubMed
description Rejection-associated gene expression has been characterized in renal allograft biopsies for cause. The aim is to evaluate rejection gene expression in subclinical rejection and in biopsies with borderline changes or interstitial fibrosis and tubular atrophy (IFTA). We included 96 biopsies. Most differentially expressed genes between normal surveillance biopsies (n = 17) and clinical rejection (n = 12) were obtained. A rejection-associated gene (RAG) score was defined as its geometric mean. The following groups were considered: (a) subclinical rejection (REJ-S, n = 6); (b) borderline changes in biopsies for cause (BL-C, n = 13); (c) borderline changes in surveillance biopsies (BL-S, n = 12); (d) IFTA in biopsies for cause (IFTA-C, n = 20); and (e) IFTA in surveillance biopsies (IFTA-S, n = 16). The outcome variable was death-censored graft loss or glomerular filtration rate decline ≥ 30 % at 2 years. A RAG score containing 109 genes derived from normal and clinical rejection (area under the curve, AUC = 1) was employed to classify the study groups. A positive RAG score was observed in 83% REJ-S, 38% BL-C, 17% BL-S, 25% IFTA-C, and 5% IFTA-S. A positive RAG score was an independent predictor of graft outcome from histological diagnosis (hazard ratio: 3.5 and 95% confidence interval: 1.1–10.9; p = 0.031). A positive RAG score predicts graft outcome in surveillance and for cause biopsies with a less severe phenotype than clinical rejection.
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spelling pubmed-76726402020-11-19 A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome Chamoun, Betty Caraben, Anna Torres, Irina B. Sellares, Joana Jiménez, Raquel Toapanta, Néstor Cidraque, Ignacio Gabaldon, Alejandra Perelló, Manel Gonzalo, Ricardo O’Valle, Francisco Moreso, Francesc Serón, Daniel Int J Mol Sci Article Rejection-associated gene expression has been characterized in renal allograft biopsies for cause. The aim is to evaluate rejection gene expression in subclinical rejection and in biopsies with borderline changes or interstitial fibrosis and tubular atrophy (IFTA). We included 96 biopsies. Most differentially expressed genes between normal surveillance biopsies (n = 17) and clinical rejection (n = 12) were obtained. A rejection-associated gene (RAG) score was defined as its geometric mean. The following groups were considered: (a) subclinical rejection (REJ-S, n = 6); (b) borderline changes in biopsies for cause (BL-C, n = 13); (c) borderline changes in surveillance biopsies (BL-S, n = 12); (d) IFTA in biopsies for cause (IFTA-C, n = 20); and (e) IFTA in surveillance biopsies (IFTA-S, n = 16). The outcome variable was death-censored graft loss or glomerular filtration rate decline ≥ 30 % at 2 years. A RAG score containing 109 genes derived from normal and clinical rejection (area under the curve, AUC = 1) was employed to classify the study groups. A positive RAG score was observed in 83% REJ-S, 38% BL-C, 17% BL-S, 25% IFTA-C, and 5% IFTA-S. A positive RAG score was an independent predictor of graft outcome from histological diagnosis (hazard ratio: 3.5 and 95% confidence interval: 1.1–10.9; p = 0.031). A positive RAG score predicts graft outcome in surveillance and for cause biopsies with a less severe phenotype than clinical rejection. MDPI 2020-11-03 /pmc/articles/PMC7672640/ /pubmed/33153205 http://dx.doi.org/10.3390/ijms21218237 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chamoun, Betty
Caraben, Anna
Torres, Irina B.
Sellares, Joana
Jiménez, Raquel
Toapanta, Néstor
Cidraque, Ignacio
Gabaldon, Alejandra
Perelló, Manel
Gonzalo, Ricardo
O’Valle, Francisco
Moreso, Francesc
Serón, Daniel
A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome
title A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome
title_full A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome
title_fullStr A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome
title_full_unstemmed A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome
title_short A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome
title_sort rejection gene expression score in indication and surveillance biopsies is associated with graft outcome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672640/
https://www.ncbi.nlm.nih.gov/pubmed/33153205
http://dx.doi.org/10.3390/ijms21218237
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