Cargando…
A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome
Rejection-associated gene expression has been characterized in renal allograft biopsies for cause. The aim is to evaluate rejection gene expression in subclinical rejection and in biopsies with borderline changes or interstitial fibrosis and tubular atrophy (IFTA). We included 96 biopsies. Most diff...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672640/ https://www.ncbi.nlm.nih.gov/pubmed/33153205 http://dx.doi.org/10.3390/ijms21218237 |
_version_ | 1783611178177527808 |
---|---|
author | Chamoun, Betty Caraben, Anna Torres, Irina B. Sellares, Joana Jiménez, Raquel Toapanta, Néstor Cidraque, Ignacio Gabaldon, Alejandra Perelló, Manel Gonzalo, Ricardo O’Valle, Francisco Moreso, Francesc Serón, Daniel |
author_facet | Chamoun, Betty Caraben, Anna Torres, Irina B. Sellares, Joana Jiménez, Raquel Toapanta, Néstor Cidraque, Ignacio Gabaldon, Alejandra Perelló, Manel Gonzalo, Ricardo O’Valle, Francisco Moreso, Francesc Serón, Daniel |
author_sort | Chamoun, Betty |
collection | PubMed |
description | Rejection-associated gene expression has been characterized in renal allograft biopsies for cause. The aim is to evaluate rejection gene expression in subclinical rejection and in biopsies with borderline changes or interstitial fibrosis and tubular atrophy (IFTA). We included 96 biopsies. Most differentially expressed genes between normal surveillance biopsies (n = 17) and clinical rejection (n = 12) were obtained. A rejection-associated gene (RAG) score was defined as its geometric mean. The following groups were considered: (a) subclinical rejection (REJ-S, n = 6); (b) borderline changes in biopsies for cause (BL-C, n = 13); (c) borderline changes in surveillance biopsies (BL-S, n = 12); (d) IFTA in biopsies for cause (IFTA-C, n = 20); and (e) IFTA in surveillance biopsies (IFTA-S, n = 16). The outcome variable was death-censored graft loss or glomerular filtration rate decline ≥ 30 % at 2 years. A RAG score containing 109 genes derived from normal and clinical rejection (area under the curve, AUC = 1) was employed to classify the study groups. A positive RAG score was observed in 83% REJ-S, 38% BL-C, 17% BL-S, 25% IFTA-C, and 5% IFTA-S. A positive RAG score was an independent predictor of graft outcome from histological diagnosis (hazard ratio: 3.5 and 95% confidence interval: 1.1–10.9; p = 0.031). A positive RAG score predicts graft outcome in surveillance and for cause biopsies with a less severe phenotype than clinical rejection. |
format | Online Article Text |
id | pubmed-7672640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76726402020-11-19 A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome Chamoun, Betty Caraben, Anna Torres, Irina B. Sellares, Joana Jiménez, Raquel Toapanta, Néstor Cidraque, Ignacio Gabaldon, Alejandra Perelló, Manel Gonzalo, Ricardo O’Valle, Francisco Moreso, Francesc Serón, Daniel Int J Mol Sci Article Rejection-associated gene expression has been characterized in renal allograft biopsies for cause. The aim is to evaluate rejection gene expression in subclinical rejection and in biopsies with borderline changes or interstitial fibrosis and tubular atrophy (IFTA). We included 96 biopsies. Most differentially expressed genes between normal surveillance biopsies (n = 17) and clinical rejection (n = 12) were obtained. A rejection-associated gene (RAG) score was defined as its geometric mean. The following groups were considered: (a) subclinical rejection (REJ-S, n = 6); (b) borderline changes in biopsies for cause (BL-C, n = 13); (c) borderline changes in surveillance biopsies (BL-S, n = 12); (d) IFTA in biopsies for cause (IFTA-C, n = 20); and (e) IFTA in surveillance biopsies (IFTA-S, n = 16). The outcome variable was death-censored graft loss or glomerular filtration rate decline ≥ 30 % at 2 years. A RAG score containing 109 genes derived from normal and clinical rejection (area under the curve, AUC = 1) was employed to classify the study groups. A positive RAG score was observed in 83% REJ-S, 38% BL-C, 17% BL-S, 25% IFTA-C, and 5% IFTA-S. A positive RAG score was an independent predictor of graft outcome from histological diagnosis (hazard ratio: 3.5 and 95% confidence interval: 1.1–10.9; p = 0.031). A positive RAG score predicts graft outcome in surveillance and for cause biopsies with a less severe phenotype than clinical rejection. MDPI 2020-11-03 /pmc/articles/PMC7672640/ /pubmed/33153205 http://dx.doi.org/10.3390/ijms21218237 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chamoun, Betty Caraben, Anna Torres, Irina B. Sellares, Joana Jiménez, Raquel Toapanta, Néstor Cidraque, Ignacio Gabaldon, Alejandra Perelló, Manel Gonzalo, Ricardo O’Valle, Francisco Moreso, Francesc Serón, Daniel A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome |
title | A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome |
title_full | A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome |
title_fullStr | A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome |
title_full_unstemmed | A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome |
title_short | A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome |
title_sort | rejection gene expression score in indication and surveillance biopsies is associated with graft outcome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672640/ https://www.ncbi.nlm.nih.gov/pubmed/33153205 http://dx.doi.org/10.3390/ijms21218237 |
work_keys_str_mv | AT chamounbetty arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT carabenanna arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT torresirinab arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT sellaresjoana arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT jimenezraquel arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT toapantanestor arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT cidraqueignacio arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT gabaldonalejandra arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT perellomanel arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT gonzaloricardo arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT ovallefrancisco arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT moresofrancesc arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT serondaniel arejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT chamounbetty rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT carabenanna rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT torresirinab rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT sellaresjoana rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT jimenezraquel rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT toapantanestor rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT cidraqueignacio rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT gabaldonalejandra rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT perellomanel rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT gonzaloricardo rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT ovallefrancisco rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT moresofrancesc rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome AT serondaniel rejectiongeneexpressionscoreinindicationandsurveillancebiopsiesisassociatedwithgraftoutcome |