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APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps

Aberrant activation of the WNT/CTNNB1 pathway is notorious in colorectal cancer (CRC). Here, we demonstrate that the expression of specific and crucial WNT signaling pathway genes is linked to disease progression in colonic adenomatous (AP) and hyperplastic (HP) polyps in an Iranian patient populati...

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Autores principales: Rezasoltani, Sama, Hadizadeh, Mahrooyeh, Golmohammadi, Mina, Nazemalhossini-Mojarad, Ehsan, Salari, Sina, Rezvani, Hamid, Asadzadeh-Aghdaei, Hamid, Ladomery, Michael, Young, Chris, Anaraki, Fakhrosadat, Almond, Sarah, Ashrafian Bonab, Maziar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672736/
https://www.ncbi.nlm.nih.gov/pubmed/33239858
http://dx.doi.org/10.1177/1176935120972383
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author Rezasoltani, Sama
Hadizadeh, Mahrooyeh
Golmohammadi, Mina
Nazemalhossini-Mojarad, Ehsan
Salari, Sina
Rezvani, Hamid
Asadzadeh-Aghdaei, Hamid
Ladomery, Michael
Young, Chris
Anaraki, Fakhrosadat
Almond, Sarah
Ashrafian Bonab, Maziar
author_facet Rezasoltani, Sama
Hadizadeh, Mahrooyeh
Golmohammadi, Mina
Nazemalhossini-Mojarad, Ehsan
Salari, Sina
Rezvani, Hamid
Asadzadeh-Aghdaei, Hamid
Ladomery, Michael
Young, Chris
Anaraki, Fakhrosadat
Almond, Sarah
Ashrafian Bonab, Maziar
author_sort Rezasoltani, Sama
collection PubMed
description Aberrant activation of the WNT/CTNNB1 pathway is notorious in colorectal cancer (CRC). Here, we demonstrate that the expression of specific and crucial WNT signaling pathway genes is linked to disease progression in colonic adenomatous (AP) and hyperplastic (HP) polyps in an Iranian patient population. Thus, we highlight potential gene expression profiles as candidate novel biomarkers for the early detection of CRC. From a 12-month study (2016-2017), 44 biopsy samples were collected during colonoscopy from the patients with colorectal polyps and 10 healthy subjects for normalization. Clinical and demographic data were collected in all cases, and mRNA expression of APC, CTNNB1, CDH1, AXIN1, and AXIN2 genes was investigated using real-time polymerase chain reaction (PCR). CTNNB1 and CDH1 expression levels were unaltered in AP and HP subjects, whereas mRNA expression of APC was decreased in AP contrasted with HP subjects, with a significant association between APC downregulation and polyp size. Although AXIN1 showed no changes between AP and HP groups, a significant association between AXIN1 and dysplasia grade was found. Also, significant upregulation of AXIN2 in both AP and HP subjects was detected. In summary, we have shown increased expression of AXIN2 and decreased expression of APC correlating with grade of dysplasia and polyp size. Hence, AXIN2 and APC should be explored as biomarker candidates for early detection of AP and HP polyps in CRC.
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spelling pubmed-76727362020-11-24 APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps Rezasoltani, Sama Hadizadeh, Mahrooyeh Golmohammadi, Mina Nazemalhossini-Mojarad, Ehsan Salari, Sina Rezvani, Hamid Asadzadeh-Aghdaei, Hamid Ladomery, Michael Young, Chris Anaraki, Fakhrosadat Almond, Sarah Ashrafian Bonab, Maziar Cancer Inform Original Research Aberrant activation of the WNT/CTNNB1 pathway is notorious in colorectal cancer (CRC). Here, we demonstrate that the expression of specific and crucial WNT signaling pathway genes is linked to disease progression in colonic adenomatous (AP) and hyperplastic (HP) polyps in an Iranian patient population. Thus, we highlight potential gene expression profiles as candidate novel biomarkers for the early detection of CRC. From a 12-month study (2016-2017), 44 biopsy samples were collected during colonoscopy from the patients with colorectal polyps and 10 healthy subjects for normalization. Clinical and demographic data were collected in all cases, and mRNA expression of APC, CTNNB1, CDH1, AXIN1, and AXIN2 genes was investigated using real-time polymerase chain reaction (PCR). CTNNB1 and CDH1 expression levels were unaltered in AP and HP subjects, whereas mRNA expression of APC was decreased in AP contrasted with HP subjects, with a significant association between APC downregulation and polyp size. Although AXIN1 showed no changes between AP and HP groups, a significant association between AXIN1 and dysplasia grade was found. Also, significant upregulation of AXIN2 in both AP and HP subjects was detected. In summary, we have shown increased expression of AXIN2 and decreased expression of APC correlating with grade of dysplasia and polyp size. Hence, AXIN2 and APC should be explored as biomarker candidates for early detection of AP and HP polyps in CRC. SAGE Publications 2020-11-11 /pmc/articles/PMC7672736/ /pubmed/33239858 http://dx.doi.org/10.1177/1176935120972383 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Rezasoltani, Sama
Hadizadeh, Mahrooyeh
Golmohammadi, Mina
Nazemalhossini-Mojarad, Ehsan
Salari, Sina
Rezvani, Hamid
Asadzadeh-Aghdaei, Hamid
Ladomery, Michael
Young, Chris
Anaraki, Fakhrosadat
Almond, Sarah
Ashrafian Bonab, Maziar
APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps
title APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps
title_full APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps
title_fullStr APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps
title_full_unstemmed APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps
title_short APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps
title_sort apc and axin2 are promising biomarker candidates for the early detection of adenomas and hyperplastic polyps
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672736/
https://www.ncbi.nlm.nih.gov/pubmed/33239858
http://dx.doi.org/10.1177/1176935120972383
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