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First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China
BACKGROUND: Virus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China. OBJECTIVE: The leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong provinc...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672823/ https://www.ncbi.nlm.nih.gov/pubmed/33208142 http://dx.doi.org/10.1186/s12985-020-01438-3 |
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author | Yang, Yang Sun, Yifan Li, Qingliang Wu, Yufeng Wang, Deya |
author_facet | Yang, Yang Sun, Yifan Li, Qingliang Wu, Yufeng Wang, Deya |
author_sort | Yang, Yang |
collection | PubMed |
description | BACKGROUND: Virus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China. OBJECTIVE: The leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong province, which the leaves suffered from viral-like symptoms with spotting and crinkle. METHODS: Small RNA-seq combined with reverse transcription PCR (RT-PCR) were performed to detect the potential viruses in these field samples. Phylogenetic tree was constructed using the neighbor joining method in MEGA 5.1 CONCLUSIONS: This is the first report of PBNSPaV infecting grapevine in China, contributing to a better understanding of the epidemiology and host range distribution of this pathogen. |
format | Online Article Text |
id | pubmed-7672823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-76728232020-11-19 First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China Yang, Yang Sun, Yifan Li, Qingliang Wu, Yufeng Wang, Deya Virol J Short Report BACKGROUND: Virus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China. OBJECTIVE: The leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong province, which the leaves suffered from viral-like symptoms with spotting and crinkle. METHODS: Small RNA-seq combined with reverse transcription PCR (RT-PCR) were performed to detect the potential viruses in these field samples. Phylogenetic tree was constructed using the neighbor joining method in MEGA 5.1 CONCLUSIONS: This is the first report of PBNSPaV infecting grapevine in China, contributing to a better understanding of the epidemiology and host range distribution of this pathogen. BioMed Central 2020-11-18 /pmc/articles/PMC7672823/ /pubmed/33208142 http://dx.doi.org/10.1186/s12985-020-01438-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Short Report Yang, Yang Sun, Yifan Li, Qingliang Wu, Yufeng Wang, Deya First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China |
title | First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China |
title_full | First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China |
title_fullStr | First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China |
title_full_unstemmed | First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China |
title_short | First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China |
title_sort | first report of plum bark necrosis stem pitting-associated virus infecting grapevine in china |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672823/ https://www.ncbi.nlm.nih.gov/pubmed/33208142 http://dx.doi.org/10.1186/s12985-020-01438-3 |
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