Cargando…

Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation

One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and effic...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Rong, Wei, Qiuhui, Wei, Xin, Liu, Yuheng, Zhang, Xiaomin, Chen, Xiabin, Yin, Xiaopu, Xie, Tian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673530/
https://www.ncbi.nlm.nih.gov/pubmed/33206717
http://dx.doi.org/10.1371/journal.pone.0242564
_version_ 1783611337621897216
author Chen, Rong
Wei, Qiuhui
Wei, Xin
Liu, Yuheng
Zhang, Xiaomin
Chen, Xiabin
Yin, Xiaopu
Xie, Tian
author_facet Chen, Rong
Wei, Qiuhui
Wei, Xin
Liu, Yuheng
Zhang, Xiaomin
Chen, Xiabin
Yin, Xiaopu
Xie, Tian
author_sort Chen, Rong
collection PubMed
description One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE.
format Online
Article
Text
id pubmed-7673530
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-76735302020-11-19 Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation Chen, Rong Wei, Qiuhui Wei, Xin Liu, Yuheng Zhang, Xiaomin Chen, Xiabin Yin, Xiaopu Xie, Tian PLoS One Research Article One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE. Public Library of Science 2020-11-18 /pmc/articles/PMC7673530/ /pubmed/33206717 http://dx.doi.org/10.1371/journal.pone.0242564 Text en © 2020 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chen, Rong
Wei, Qiuhui
Wei, Xin
Liu, Yuheng
Zhang, Xiaomin
Chen, Xiabin
Yin, Xiaopu
Xie, Tian
Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
title Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
title_full Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
title_fullStr Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
title_full_unstemmed Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
title_short Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
title_sort stable and efficient immobilization of bi-enzymatic nadph cofactor recycling system under consecutive microwave irradiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673530/
https://www.ncbi.nlm.nih.gov/pubmed/33206717
http://dx.doi.org/10.1371/journal.pone.0242564
work_keys_str_mv AT chenrong stableandefficientimmobilizationofbienzymaticnadphcofactorrecyclingsystemunderconsecutivemicrowaveirradiation
AT weiqiuhui stableandefficientimmobilizationofbienzymaticnadphcofactorrecyclingsystemunderconsecutivemicrowaveirradiation
AT weixin stableandefficientimmobilizationofbienzymaticnadphcofactorrecyclingsystemunderconsecutivemicrowaveirradiation
AT liuyuheng stableandefficientimmobilizationofbienzymaticnadphcofactorrecyclingsystemunderconsecutivemicrowaveirradiation
AT zhangxiaomin stableandefficientimmobilizationofbienzymaticnadphcofactorrecyclingsystemunderconsecutivemicrowaveirradiation
AT chenxiabin stableandefficientimmobilizationofbienzymaticnadphcofactorrecyclingsystemunderconsecutivemicrowaveirradiation
AT yinxiaopu stableandefficientimmobilizationofbienzymaticnadphcofactorrecyclingsystemunderconsecutivemicrowaveirradiation
AT xietian stableandefficientimmobilizationofbienzymaticnadphcofactorrecyclingsystemunderconsecutivemicrowaveirradiation