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Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and effic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673530/ https://www.ncbi.nlm.nih.gov/pubmed/33206717 http://dx.doi.org/10.1371/journal.pone.0242564 |
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author | Chen, Rong Wei, Qiuhui Wei, Xin Liu, Yuheng Zhang, Xiaomin Chen, Xiabin Yin, Xiaopu Xie, Tian |
author_facet | Chen, Rong Wei, Qiuhui Wei, Xin Liu, Yuheng Zhang, Xiaomin Chen, Xiabin Yin, Xiaopu Xie, Tian |
author_sort | Chen, Rong |
collection | PubMed |
description | One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE. |
format | Online Article Text |
id | pubmed-7673530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76735302020-11-19 Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation Chen, Rong Wei, Qiuhui Wei, Xin Liu, Yuheng Zhang, Xiaomin Chen, Xiabin Yin, Xiaopu Xie, Tian PLoS One Research Article One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE. Public Library of Science 2020-11-18 /pmc/articles/PMC7673530/ /pubmed/33206717 http://dx.doi.org/10.1371/journal.pone.0242564 Text en © 2020 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Chen, Rong Wei, Qiuhui Wei, Xin Liu, Yuheng Zhang, Xiaomin Chen, Xiabin Yin, Xiaopu Xie, Tian Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation |
title | Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation |
title_full | Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation |
title_fullStr | Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation |
title_full_unstemmed | Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation |
title_short | Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation |
title_sort | stable and efficient immobilization of bi-enzymatic nadph cofactor recycling system under consecutive microwave irradiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673530/ https://www.ncbi.nlm.nih.gov/pubmed/33206717 http://dx.doi.org/10.1371/journal.pone.0242564 |
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