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Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders
Currently, considering cryopreservation of bull semen, there is no clear consensus over the comparability of cryoprotective efficacy of extenders with soybean lecithin and those based on egg yolk. The objective of this study was to prove the use of Low Density Lipoprotein (LDL) extracted from hen-eg...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Reprodução Animal - CBRA
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673600/ https://www.ncbi.nlm.nih.gov/pubmed/33224286 http://dx.doi.org/10.21451/1984-3143-AR2018-0107 |
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author | Ondřej, Šimoník Jiří, Šichtař Jan, Beran Pavla, Maňásková-Postlerová Lucie, Tůmová Doležalová, Martina Petra, Folková Luděk, Stádník Radko, Rajmon |
author_facet | Ondřej, Šimoník Jiří, Šichtař Jan, Beran Pavla, Maňásková-Postlerová Lucie, Tůmová Doležalová, Martina Petra, Folková Luděk, Stádník Radko, Rajmon |
author_sort | Ondřej, Šimoník |
collection | PubMed |
description | Currently, considering cryopreservation of bull semen, there is no clear consensus over the comparability of cryoprotective efficacy of extenders with soybean lecithin and those based on egg yolk. The objective of this study was to prove the use of Low Density Lipoprotein (LDL) extracted from hen-egg yolk as an enhancing factor for soybean lecithin-based extenders. In total, 35 ejaculates of (seven bulls x five ejaculates per bull) were collected and cryopreserved at a commercial insemination centre. The effect of the LDL addition to the extenders AndroMed(®) and Bioxcell(®) was tested in a 6% (v/v) concentration on spermatozoa after thawing. Modified extender composition effects were assessed on sperm functional parameters motility, plasma membrane, mitochondrial membrane potential and acrosomal integrity after thawing by CASA, flow cytometry and fluorescent microscopy, respectively. Based on kinematic parameters determined from CASA, k-means cluster analysis was used to classify individual spermatozoon into specific subpopulations (fast, medium fast and slow). A subpopulation of fast spermatozoa was increased in the presence of LDL in both selected extenders (P < 0.05). Moreover, the positive effect of LDL on sperm motility was confirmed by decreasing the percentage of sperm in slow subpopulation (P < 0.05). The effect of LDL addition on the incidence of spermatozoa with intact plasma membrane was not demonstrated in any case of extender used (P > 0.05). The percentage of sperm with intact acrosome was improved when LDL was added to Bioxcell(®) extender (P < 0.05). On the other hand, addition of LDL to AndroMed(®) extender improved mitochondrial intactness after thawing (P < 0.05). In conclusion, our results showed that adding LDL to selected soybean lecithin-based extenders considerably ameliorated the functional parameters of spermatozoa after thawing and thus this lipoprotein could represent an improving agent for soybean lecithin-based extender for bull semen cryopreservation. |
format | Online Article Text |
id | pubmed-7673600 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Colégio Brasileiro de Reprodução Animal - CBRA |
record_format | MEDLINE/PubMed |
spelling | pubmed-76736002020-11-20 Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders Ondřej, Šimoník Jiří, Šichtař Jan, Beran Pavla, Maňásková-Postlerová Lucie, Tůmová Doležalová, Martina Petra, Folková Luděk, Stádník Radko, Rajmon Anim Reprod Original Article Currently, considering cryopreservation of bull semen, there is no clear consensus over the comparability of cryoprotective efficacy of extenders with soybean lecithin and those based on egg yolk. The objective of this study was to prove the use of Low Density Lipoprotein (LDL) extracted from hen-egg yolk as an enhancing factor for soybean lecithin-based extenders. In total, 35 ejaculates of (seven bulls x five ejaculates per bull) were collected and cryopreserved at a commercial insemination centre. The effect of the LDL addition to the extenders AndroMed(®) and Bioxcell(®) was tested in a 6% (v/v) concentration on spermatozoa after thawing. Modified extender composition effects were assessed on sperm functional parameters motility, plasma membrane, mitochondrial membrane potential and acrosomal integrity after thawing by CASA, flow cytometry and fluorescent microscopy, respectively. Based on kinematic parameters determined from CASA, k-means cluster analysis was used to classify individual spermatozoon into specific subpopulations (fast, medium fast and slow). A subpopulation of fast spermatozoa was increased in the presence of LDL in both selected extenders (P < 0.05). Moreover, the positive effect of LDL on sperm motility was confirmed by decreasing the percentage of sperm in slow subpopulation (P < 0.05). The effect of LDL addition on the incidence of spermatozoa with intact plasma membrane was not demonstrated in any case of extender used (P > 0.05). The percentage of sperm with intact acrosome was improved when LDL was added to Bioxcell(®) extender (P < 0.05). On the other hand, addition of LDL to AndroMed(®) extender improved mitochondrial intactness after thawing (P < 0.05). In conclusion, our results showed that adding LDL to selected soybean lecithin-based extenders considerably ameliorated the functional parameters of spermatozoa after thawing and thus this lipoprotein could represent an improving agent for soybean lecithin-based extender for bull semen cryopreservation. Colégio Brasileiro de Reprodução Animal - CBRA 2019-10-23 /pmc/articles/PMC7673600/ /pubmed/33224286 http://dx.doi.org/10.21451/1984-3143-AR2018-0107 Text en Copyright © The Author(s). Published by CBRA. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article under the Creative Commons Attribution License (CC BY 4.0 license) |
spellingShingle | Original Article Ondřej, Šimoník Jiří, Šichtař Jan, Beran Pavla, Maňásková-Postlerová Lucie, Tůmová Doležalová, Martina Petra, Folková Luděk, Stádník Radko, Rajmon Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders |
title | Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders |
title_full | Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders |
title_fullStr | Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders |
title_full_unstemmed | Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders |
title_short | Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders |
title_sort | low density lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673600/ https://www.ncbi.nlm.nih.gov/pubmed/33224286 http://dx.doi.org/10.21451/1984-3143-AR2018-0107 |
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