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Monodisperse drops templated by 3D-structured microparticles

The ability to create uniform subnanoliter compartments using microfluidic control has enabled new approaches for analysis of single cells and molecules. However, specialized instruments or expertise has been required, slowing the adoption of these cutting-edge applications. Here, we show that three...

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Autores principales: Wu, Chueh-Yu, Ouyang, Mengxing, Wang, Bao, de Rutte, Joseph, Joo, Alexis, Jacobs, Matthew, Ha, Kyung, Bertozzi, Andrea L., Di Carlo, Dino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673687/
https://www.ncbi.nlm.nih.gov/pubmed/33148643
http://dx.doi.org/10.1126/sciadv.abb9023
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author Wu, Chueh-Yu
Ouyang, Mengxing
Wang, Bao
de Rutte, Joseph
Joo, Alexis
Jacobs, Matthew
Ha, Kyung
Bertozzi, Andrea L.
Di Carlo, Dino
author_facet Wu, Chueh-Yu
Ouyang, Mengxing
Wang, Bao
de Rutte, Joseph
Joo, Alexis
Jacobs, Matthew
Ha, Kyung
Bertozzi, Andrea L.
Di Carlo, Dino
author_sort Wu, Chueh-Yu
collection PubMed
description The ability to create uniform subnanoliter compartments using microfluidic control has enabled new approaches for analysis of single cells and molecules. However, specialized instruments or expertise has been required, slowing the adoption of these cutting-edge applications. Here, we show that three dimensional–structured microparticles with sculpted surface chemistries template uniformly sized aqueous drops when simply mixed with two immiscible fluid phases. In contrast to traditional emulsions, particle-templated drops of a controlled volume occupy a minimum in the interfacial energy of the system, such that a stable monodisperse state results with simple and reproducible formation conditions. We describe techniques to manufacture microscale drop-carrier particles and show that emulsions created with these particles prevent molecular exchange, concentrating reactions within the drops, laying a foundation for sensitive compartmentalized molecular and cell-based assays with minimal instrumentation.
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spelling pubmed-76736872020-11-24 Monodisperse drops templated by 3D-structured microparticles Wu, Chueh-Yu Ouyang, Mengxing Wang, Bao de Rutte, Joseph Joo, Alexis Jacobs, Matthew Ha, Kyung Bertozzi, Andrea L. Di Carlo, Dino Sci Adv Research Articles The ability to create uniform subnanoliter compartments using microfluidic control has enabled new approaches for analysis of single cells and molecules. However, specialized instruments or expertise has been required, slowing the adoption of these cutting-edge applications. Here, we show that three dimensional–structured microparticles with sculpted surface chemistries template uniformly sized aqueous drops when simply mixed with two immiscible fluid phases. In contrast to traditional emulsions, particle-templated drops of a controlled volume occupy a minimum in the interfacial energy of the system, such that a stable monodisperse state results with simple and reproducible formation conditions. We describe techniques to manufacture microscale drop-carrier particles and show that emulsions created with these particles prevent molecular exchange, concentrating reactions within the drops, laying a foundation for sensitive compartmentalized molecular and cell-based assays with minimal instrumentation. American Association for the Advancement of Science 2020-11-04 /pmc/articles/PMC7673687/ /pubmed/33148643 http://dx.doi.org/10.1126/sciadv.abb9023 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Wu, Chueh-Yu
Ouyang, Mengxing
Wang, Bao
de Rutte, Joseph
Joo, Alexis
Jacobs, Matthew
Ha, Kyung
Bertozzi, Andrea L.
Di Carlo, Dino
Monodisperse drops templated by 3D-structured microparticles
title Monodisperse drops templated by 3D-structured microparticles
title_full Monodisperse drops templated by 3D-structured microparticles
title_fullStr Monodisperse drops templated by 3D-structured microparticles
title_full_unstemmed Monodisperse drops templated by 3D-structured microparticles
title_short Monodisperse drops templated by 3D-structured microparticles
title_sort monodisperse drops templated by 3d-structured microparticles
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673687/
https://www.ncbi.nlm.nih.gov/pubmed/33148643
http://dx.doi.org/10.1126/sciadv.abb9023
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