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Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative
The early detection and differential diagnosis of respiratory infections increase the chances for successful control of COVID-19 disease. The nucleic acid RT-PCR test is regarded as the current standard for molecular diagnosis. However, the maximal specificity confirmation target ORF1ab gene is cons...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673793/ https://www.ncbi.nlm.nih.gov/pubmed/33206690 http://dx.doi.org/10.1371/journal.pone.0241469 |
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author | Zhou, Yunying Pei, Fengyan Ji, Mingyu Wang, Li Zhao, Huailong Li, Huanjie Yang, Weihua Wang, Qingxi Zhao, Qianqian Wang, Yunshan |
author_facet | Zhou, Yunying Pei, Fengyan Ji, Mingyu Wang, Li Zhao, Huailong Li, Huanjie Yang, Weihua Wang, Qingxi Zhao, Qianqian Wang, Yunshan |
author_sort | Zhou, Yunying |
collection | PubMed |
description | The early detection and differential diagnosis of respiratory infections increase the chances for successful control of COVID-19 disease. The nucleic acid RT-PCR test is regarded as the current standard for molecular diagnosis. However, the maximal specificity confirmation target ORF1ab gene is considered to be less sensitive than other targets in clinical application. In addition, recent evidence indicated that the initial missed diagnosis of asymptomatic patients with SARS-CoV-2 and discharged patients with “re-examination positive” might be due to low viral load, and the ability of rapid mutation of SARS-CoV-2 also increases the rate of false-negative results. Moreover, the mixed sample nucleic acid detection is helpful in seeking out the early community transmission of SARS-CoV-2 rapidly, but the detection kit needs ultra-high detection sensitivity. Herein, the lowest detection concentration of different nucleic acid detection kits was evaluated and compared to provide direct evidence for the selection of kits for mixed sample detection or make recommendations for the selection of validation kit, which is of great significance for the prevention and control of the current epidemic and the discharge criteria of low viral load patients. |
format | Online Article Text |
id | pubmed-7673793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76737932020-11-19 Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative Zhou, Yunying Pei, Fengyan Ji, Mingyu Wang, Li Zhao, Huailong Li, Huanjie Yang, Weihua Wang, Qingxi Zhao, Qianqian Wang, Yunshan PLoS One Research Article The early detection and differential diagnosis of respiratory infections increase the chances for successful control of COVID-19 disease. The nucleic acid RT-PCR test is regarded as the current standard for molecular diagnosis. However, the maximal specificity confirmation target ORF1ab gene is considered to be less sensitive than other targets in clinical application. In addition, recent evidence indicated that the initial missed diagnosis of asymptomatic patients with SARS-CoV-2 and discharged patients with “re-examination positive” might be due to low viral load, and the ability of rapid mutation of SARS-CoV-2 also increases the rate of false-negative results. Moreover, the mixed sample nucleic acid detection is helpful in seeking out the early community transmission of SARS-CoV-2 rapidly, but the detection kit needs ultra-high detection sensitivity. Herein, the lowest detection concentration of different nucleic acid detection kits was evaluated and compared to provide direct evidence for the selection of kits for mixed sample detection or make recommendations for the selection of validation kit, which is of great significance for the prevention and control of the current epidemic and the discharge criteria of low viral load patients. Public Library of Science 2020-11-18 /pmc/articles/PMC7673793/ /pubmed/33206690 http://dx.doi.org/10.1371/journal.pone.0241469 Text en © 2020 Zhou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhou, Yunying Pei, Fengyan Ji, Mingyu Wang, Li Zhao, Huailong Li, Huanjie Yang, Weihua Wang, Qingxi Zhao, Qianqian Wang, Yunshan Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative |
title | Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative |
title_full | Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative |
title_fullStr | Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative |
title_full_unstemmed | Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative |
title_short | Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative |
title_sort | sensitivity evaluation of 2019 novel coronavirus (sars-cov-2) rt-pcr detection kits and strategy to reduce false negative |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673793/ https://www.ncbi.nlm.nih.gov/pubmed/33206690 http://dx.doi.org/10.1371/journal.pone.0241469 |
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