The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella

Comparative genomics identified the environmental bacterial genus Shinella as the most likely origin of the class A carbapenemases BKC-1 and GPC-1. Available sequences and PCR analyses of additional Shinella species revealed homologous β-lactamases showing up to 85.4% and 93.3% amino acid identity t...

Descripción completa

Detalles Bibliográficos
Autores principales: Kieffer, Nicolas, Ebmeyer, Stefan, Larsson, D. G. Joakim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Mechanisms of Resistance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674036/
https://www.ncbi.nlm.nih.gov/pubmed/32958716
http://dx.doi.org/10.1128/AAC.01263-20
Descripción
Sumario:Comparative genomics identified the environmental bacterial genus Shinella as the most likely origin of the class A carbapenemases BKC-1 and GPC-1. Available sequences and PCR analyses of additional Shinella species revealed homologous β-lactamases showing up to 85.4% and 93.3% amino acid identity to both enzymes, respectively. The genes conferred resistance to β-lactams once expressed in Escherichia coli. bla(BKC-1) likely evolved from a putative ancestral Shinella gene with higher homology through duplication of a gene fragment.

Ejemplares similares