The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella

Comparative genomics identified the environmental bacterial genus Shinella as the most likely origin of the class A carbapenemases BKC-1 and GPC-1. Available sequences and PCR analyses of additional Shinella species revealed homologous β-lactamases showing up to 85.4% and 93.3% amino acid identity t...

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Detalles Bibliográficos
Autores principales: Kieffer, Nicolas, Ebmeyer, Stefan, Larsson, D. G. Joakim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Mechanisms of Resistance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674036/
https://www.ncbi.nlm.nih.gov/pubmed/32958716
http://dx.doi.org/10.1128/AAC.01263-20
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author Kieffer, Nicolas
Ebmeyer, Stefan
Larsson, D. G. Joakim
author_facet Kieffer, Nicolas
Ebmeyer, Stefan
Larsson, D. G. Joakim
author_sort Kieffer, Nicolas
collection PubMed
description Comparative genomics identified the environmental bacterial genus Shinella as the most likely origin of the class A carbapenemases BKC-1 and GPC-1. Available sequences and PCR analyses of additional Shinella species revealed homologous β-lactamases showing up to 85.4% and 93.3% amino acid identity to both enzymes, respectively. The genes conferred resistance to β-lactams once expressed in Escherichia coli. bla(BKC-1) likely evolved from a putative ancestral Shinella gene with higher homology through duplication of a gene fragment.
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spelling pubmed-76740362020-12-09 The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella Kieffer, Nicolas Ebmeyer, Stefan Larsson, D. G. Joakim Antimicrob Agents Chemother Mechanisms of Resistance Comparative genomics identified the environmental bacterial genus Shinella as the most likely origin of the class A carbapenemases BKC-1 and GPC-1. Available sequences and PCR analyses of additional Shinella species revealed homologous β-lactamases showing up to 85.4% and 93.3% amino acid identity to both enzymes, respectively. The genes conferred resistance to β-lactams once expressed in Escherichia coli. bla(BKC-1) likely evolved from a putative ancestral Shinella gene with higher homology through duplication of a gene fragment. American Society for Microbiology 2020-11-17 /pmc/articles/PMC7674036/ /pubmed/32958716 http://dx.doi.org/10.1128/AAC.01263-20 Text en Copyright © 2020 Kieffer et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Mechanisms of Resistance
Kieffer, Nicolas
Ebmeyer, Stefan
Larsson, D. G. Joakim
The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella
title The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella
title_full The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella
title_fullStr The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella
title_full_unstemmed The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella
title_short The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella
title_sort class a carbapenemases bkc-1 and gpc-1 both originate from the bacterial genus shinella
topic Mechanisms of Resistance
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674036/
https://www.ncbi.nlm.nih.gov/pubmed/32958716
http://dx.doi.org/10.1128/AAC.01263-20
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