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Antimicrobial assessment of polyphenolic extracts from onion (Allium cepa L.) skin of fifteen cultivars by sonication-assisted extraction method
Onion skin possesses various health benefits due to its phenolic and antimicrobial components. In this study, sonication-assisted extracts of onion skin of differentially coloured cultivars (dark-red, red, pink and white) were investigated for their antimicrobial activity against six pathogenic bact...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674300/ https://www.ncbi.nlm.nih.gov/pubmed/33241150 http://dx.doi.org/10.1016/j.heliyon.2020.e05478 |
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author | Sagar, Narashans Alok Pareek, Sunil |
author_facet | Sagar, Narashans Alok Pareek, Sunil |
author_sort | Sagar, Narashans Alok |
collection | PubMed |
description | Onion skin possesses various health benefits due to its phenolic and antimicrobial components. In this study, sonication-assisted extracts of onion skin of differentially coloured cultivars (dark-red, red, pink and white) were investigated for their antimicrobial activity against six pathogenic bacteria. Antimicrobial efficacy of fifteen different coloured extracts was analysed by agar well-diffusion assay with principal component analysis (PCA) for comprehensive investigation. Result showed skin extracts of pink cultivars (cv.) significantly (P ≤ 0.05) effective against pathogenic bacteria followed by red and dark red skin. White skin showed least effect on the growth of bacteria. Skin of cv. ‘Phursungi Local’ (pink) and cv. ‘Hissar-3’ (pink) showed best range of inhibition against Staphylococcus aureus, Klebsiella pnuemoniae, Bacillus cereus and Solmonella typhimurium compared to other. Only white skin extracts of cv. ‘Bhima Shubhra’ and ‘Udaipur Local’ inhibited the growth of Pseudomonas aeruginosa up to 4.0 ± 0.0 mm. Minimum inhibitory concentration (MIC) of the effective extracts was also elucidated in the range between 0.09 – 9.0 mg/mL. Skin extracts of cv. ‘Hissar-2’ (red) and ‘Bhima Shubhra’ (white) showed better inhibition at the concentration of 0.45 and 0.72 mg/mL against Streptococcus agalactiae and Pseudomonas aeruginosa, respectively. As per correlation analysis, positive correlation was obtained between total flavonoids and inhibition rate of all the bacteria while a weak correlation (R(2) = 0.3967) was observed against Pseudomonas aeruginosa. The waste skin of the analysed cultivars can be utilised in food and health sector as natural preservative and antimicrobial agent. |
format | Online Article Text |
id | pubmed-7674300 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-76743002020-11-24 Antimicrobial assessment of polyphenolic extracts from onion (Allium cepa L.) skin of fifteen cultivars by sonication-assisted extraction method Sagar, Narashans Alok Pareek, Sunil Heliyon Research Article Onion skin possesses various health benefits due to its phenolic and antimicrobial components. In this study, sonication-assisted extracts of onion skin of differentially coloured cultivars (dark-red, red, pink and white) were investigated for their antimicrobial activity against six pathogenic bacteria. Antimicrobial efficacy of fifteen different coloured extracts was analysed by agar well-diffusion assay with principal component analysis (PCA) for comprehensive investigation. Result showed skin extracts of pink cultivars (cv.) significantly (P ≤ 0.05) effective against pathogenic bacteria followed by red and dark red skin. White skin showed least effect on the growth of bacteria. Skin of cv. ‘Phursungi Local’ (pink) and cv. ‘Hissar-3’ (pink) showed best range of inhibition against Staphylococcus aureus, Klebsiella pnuemoniae, Bacillus cereus and Solmonella typhimurium compared to other. Only white skin extracts of cv. ‘Bhima Shubhra’ and ‘Udaipur Local’ inhibited the growth of Pseudomonas aeruginosa up to 4.0 ± 0.0 mm. Minimum inhibitory concentration (MIC) of the effective extracts was also elucidated in the range between 0.09 – 9.0 mg/mL. Skin extracts of cv. ‘Hissar-2’ (red) and ‘Bhima Shubhra’ (white) showed better inhibition at the concentration of 0.45 and 0.72 mg/mL against Streptococcus agalactiae and Pseudomonas aeruginosa, respectively. As per correlation analysis, positive correlation was obtained between total flavonoids and inhibition rate of all the bacteria while a weak correlation (R(2) = 0.3967) was observed against Pseudomonas aeruginosa. The waste skin of the analysed cultivars can be utilised in food and health sector as natural preservative and antimicrobial agent. Elsevier 2020-11-12 /pmc/articles/PMC7674300/ /pubmed/33241150 http://dx.doi.org/10.1016/j.heliyon.2020.e05478 Text en © 2020 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Sagar, Narashans Alok Pareek, Sunil Antimicrobial assessment of polyphenolic extracts from onion (Allium cepa L.) skin of fifteen cultivars by sonication-assisted extraction method |
title | Antimicrobial assessment of polyphenolic extracts from onion (Allium cepa L.) skin of fifteen cultivars by sonication-assisted extraction method |
title_full | Antimicrobial assessment of polyphenolic extracts from onion (Allium cepa L.) skin of fifteen cultivars by sonication-assisted extraction method |
title_fullStr | Antimicrobial assessment of polyphenolic extracts from onion (Allium cepa L.) skin of fifteen cultivars by sonication-assisted extraction method |
title_full_unstemmed | Antimicrobial assessment of polyphenolic extracts from onion (Allium cepa L.) skin of fifteen cultivars by sonication-assisted extraction method |
title_short | Antimicrobial assessment of polyphenolic extracts from onion (Allium cepa L.) skin of fifteen cultivars by sonication-assisted extraction method |
title_sort | antimicrobial assessment of polyphenolic extracts from onion (allium cepa l.) skin of fifteen cultivars by sonication-assisted extraction method |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674300/ https://www.ncbi.nlm.nih.gov/pubmed/33241150 http://dx.doi.org/10.1016/j.heliyon.2020.e05478 |
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