Solution structure of Gaussia Luciferase with five disulfide bonds and identification of a putative coelenterazine binding cavity by heteronuclear NMR

Gaussia luciferase (GLuc) is a small luciferase (18.2 kDa; 168 residues) and is thus attracting much attention as a reporter protein, but the lack of structural information is hampering further application. Here, we report the first solution structure of a fully active, recombinant GLuc determined b...

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Detalles Bibliográficos
Autores principales: Wu, Nan, Kobayashi, Naohiro, Tsuda, Kengo, Unzai, Satoru, Saotome, Tomonori, Kuroda, Yutaka, Yamazaki, Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674443/
https://www.ncbi.nlm.nih.gov/pubmed/33208800
http://dx.doi.org/10.1038/s41598-020-76486-4

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