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DCX(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus

A pool of different types of neural progenitor cells resides in the adult hippocampus. Apart from doublecortin-expressing (DCX(+)) neuronal progenitor cells (NPCs), the hippocampal parenchyma also contains oligodendrocyte precursor cells (OPCs), which can differentiate into myelinating oligodendrocy...

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Autores principales: Klein, Barbara, Mrowetz, Heike, Kreutzer, Christina, Rotheneichner, Peter, Zaunmair, Pia, Lange, Simona, Coras, Roland, Couillard-Despres, Sebastien, Rivera, Francisco J., Aigner, Ludwig
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674453/
https://www.ncbi.nlm.nih.gov/pubmed/33208869
http://dx.doi.org/10.1038/s41598-020-77115-w
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author Klein, Barbara
Mrowetz, Heike
Kreutzer, Christina
Rotheneichner, Peter
Zaunmair, Pia
Lange, Simona
Coras, Roland
Couillard-Despres, Sebastien
Rivera, Francisco J.
Aigner, Ludwig
author_facet Klein, Barbara
Mrowetz, Heike
Kreutzer, Christina
Rotheneichner, Peter
Zaunmair, Pia
Lange, Simona
Coras, Roland
Couillard-Despres, Sebastien
Rivera, Francisco J.
Aigner, Ludwig
author_sort Klein, Barbara
collection PubMed
description A pool of different types of neural progenitor cells resides in the adult hippocampus. Apart from doublecortin-expressing (DCX(+)) neuronal progenitor cells (NPCs), the hippocampal parenchyma also contains oligodendrocyte precursor cells (OPCs), which can differentiate into myelinating oligodendrocytes. It is not clear yet to what extent the functions of these different progenitor cell types overlap and how plastic these cells are in response to pathological processes. The aim of this study was to investigate whether hippocampal DCX(+) NPCs can generate new oligodendrocytes under conditions in which myelin repair is required. For this, the cell fate of DCX-expressing NPCs was analyzed during cuprizone-induced demyelination and subsequent remyelination in two regions of the hippocampal dentate gyrus of DCX-CreER(T2)/Flox-EGFP transgenic mice. In this DCX reporter model, the number of GFP(+) NPCs co-expressing Olig2 and CC1, a combination of markers typically found in mature oligodendrocytes, was significantly increased in the hippocampal DG during remyelination. In contrast, the numbers of GFP(+)PDGFRα(+) cells, as well as their proliferation, were unaffected by de- or remyelination. During remyelination, a higher portion of newly generated BrdU-labeled cells were GFP(+) NPCs and there was an increase in new oligodendrocytes derived from these proliferating cells (GFP(+)Olig2(+)BrdU(+)). These results suggest that DCX-expressing NPCs were able to contribute to the generation of mature oligodendrocytes during remyelination in the adult hippocampus.
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spelling pubmed-76744532020-11-19 DCX(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus Klein, Barbara Mrowetz, Heike Kreutzer, Christina Rotheneichner, Peter Zaunmair, Pia Lange, Simona Coras, Roland Couillard-Despres, Sebastien Rivera, Francisco J. Aigner, Ludwig Sci Rep Article A pool of different types of neural progenitor cells resides in the adult hippocampus. Apart from doublecortin-expressing (DCX(+)) neuronal progenitor cells (NPCs), the hippocampal parenchyma also contains oligodendrocyte precursor cells (OPCs), which can differentiate into myelinating oligodendrocytes. It is not clear yet to what extent the functions of these different progenitor cell types overlap and how plastic these cells are in response to pathological processes. The aim of this study was to investigate whether hippocampal DCX(+) NPCs can generate new oligodendrocytes under conditions in which myelin repair is required. For this, the cell fate of DCX-expressing NPCs was analyzed during cuprizone-induced demyelination and subsequent remyelination in two regions of the hippocampal dentate gyrus of DCX-CreER(T2)/Flox-EGFP transgenic mice. In this DCX reporter model, the number of GFP(+) NPCs co-expressing Olig2 and CC1, a combination of markers typically found in mature oligodendrocytes, was significantly increased in the hippocampal DG during remyelination. In contrast, the numbers of GFP(+)PDGFRα(+) cells, as well as their proliferation, were unaffected by de- or remyelination. During remyelination, a higher portion of newly generated BrdU-labeled cells were GFP(+) NPCs and there was an increase in new oligodendrocytes derived from these proliferating cells (GFP(+)Olig2(+)BrdU(+)). These results suggest that DCX-expressing NPCs were able to contribute to the generation of mature oligodendrocytes during remyelination in the adult hippocampus. Nature Publishing Group UK 2020-11-18 /pmc/articles/PMC7674453/ /pubmed/33208869 http://dx.doi.org/10.1038/s41598-020-77115-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Klein, Barbara
Mrowetz, Heike
Kreutzer, Christina
Rotheneichner, Peter
Zaunmair, Pia
Lange, Simona
Coras, Roland
Couillard-Despres, Sebastien
Rivera, Francisco J.
Aigner, Ludwig
DCX(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus
title DCX(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus
title_full DCX(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus
title_fullStr DCX(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus
title_full_unstemmed DCX(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus
title_short DCX(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus
title_sort dcx(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674453/
https://www.ncbi.nlm.nih.gov/pubmed/33208869
http://dx.doi.org/10.1038/s41598-020-77115-w
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