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Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability

The mutation in postoperative plasma (molecular residues) was an independently prognostic factor in colorectal cancer (CRC). The status of postoperative plasma mutation of microsatellite instability (MSI) CRC has not been systematically examined. In this study, we enrolled 30 MSI and 46 microsatelli...

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Autores principales: Li, Liren, Zhou, Wenhao, Li, Qian, Li, Pansong, Yang, Ling, Xia, Xuefeng, Yi, Xin, Wan, Desen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674603/
https://www.ncbi.nlm.nih.gov/pubmed/33190041
http://dx.doi.org/10.1016/j.tranon.2020.100945
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author Li, Liren
Zhou, Wenhao
Li, Qian
Li, Pansong
Yang, Ling
Xia, Xuefeng
Yi, Xin
Wan, Desen
author_facet Li, Liren
Zhou, Wenhao
Li, Qian
Li, Pansong
Yang, Ling
Xia, Xuefeng
Yi, Xin
Wan, Desen
author_sort Li, Liren
collection PubMed
description The mutation in postoperative plasma (molecular residues) was an independently prognostic factor in colorectal cancer (CRC). The status of postoperative plasma mutation of microsatellite instability (MSI) CRC has not been systematically examined. In this study, we enrolled 30 MSI and 46 microsatellite stability (MSS) CRCs, and performed next generation sequencing on surgical tissues, postoperative plasma, and plasma during follow-up. Compared with MSS, MSI tumors had dissimilar genomic profiles, higher tumor mutation burden (TMB), and more frameshift mutations. In the postoperative plasma, more MSI CRCs were detected with tumor-derived mutations (77% in MSI vs 33% in MSS, p < 0.001). The numbers of postoperative mutations were proportional to MSI tissues (Spearman r = 0.47, p = 0.023), while not for MSS. More proportion of postoperative plasma samples of MSI CRCs harbored frameshift mutations than MSS (p = 0.007). For the follow-up plasma, 93% (14 out of 15) MSI CRCs harbored tumor-derived mutations; 33% (4/12) MSS were mutation-positive, lower than MSI (p = 0.003). Thus, considering that MSI CRC had extremely distinct mutational characteristics in tumor and postoperative plasma compared with MSS CRC, we propose that the prognostic value of molecular residue identification in postoperative plasma needs to be independently evaluated in MSI and MSS CRCs.
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spelling pubmed-76746032020-12-07 Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability Li, Liren Zhou, Wenhao Li, Qian Li, Pansong Yang, Ling Xia, Xuefeng Yi, Xin Wan, Desen Transl Oncol Original article The mutation in postoperative plasma (molecular residues) was an independently prognostic factor in colorectal cancer (CRC). The status of postoperative plasma mutation of microsatellite instability (MSI) CRC has not been systematically examined. In this study, we enrolled 30 MSI and 46 microsatellite stability (MSS) CRCs, and performed next generation sequencing on surgical tissues, postoperative plasma, and plasma during follow-up. Compared with MSS, MSI tumors had dissimilar genomic profiles, higher tumor mutation burden (TMB), and more frameshift mutations. In the postoperative plasma, more MSI CRCs were detected with tumor-derived mutations (77% in MSI vs 33% in MSS, p < 0.001). The numbers of postoperative mutations were proportional to MSI tissues (Spearman r = 0.47, p = 0.023), while not for MSS. More proportion of postoperative plasma samples of MSI CRCs harbored frameshift mutations than MSS (p = 0.007). For the follow-up plasma, 93% (14 out of 15) MSI CRCs harbored tumor-derived mutations; 33% (4/12) MSS were mutation-positive, lower than MSI (p = 0.003). Thus, considering that MSI CRC had extremely distinct mutational characteristics in tumor and postoperative plasma compared with MSS CRC, we propose that the prognostic value of molecular residue identification in postoperative plasma needs to be independently evaluated in MSI and MSS CRCs. Neoplasia Press 2020-11-12 /pmc/articles/PMC7674603/ /pubmed/33190041 http://dx.doi.org/10.1016/j.tranon.2020.100945 Text en © 2020 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original article
Li, Liren
Zhou, Wenhao
Li, Qian
Li, Pansong
Yang, Ling
Xia, Xuefeng
Yi, Xin
Wan, Desen
Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability
title Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability
title_full Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability
title_fullStr Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability
title_full_unstemmed Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability
title_short Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability
title_sort tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability
topic Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674603/
https://www.ncbi.nlm.nih.gov/pubmed/33190041
http://dx.doi.org/10.1016/j.tranon.2020.100945
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