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Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability
The mutation in postoperative plasma (molecular residues) was an independently prognostic factor in colorectal cancer (CRC). The status of postoperative plasma mutation of microsatellite instability (MSI) CRC has not been systematically examined. In this study, we enrolled 30 MSI and 46 microsatelli...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Neoplasia Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674603/ https://www.ncbi.nlm.nih.gov/pubmed/33190041 http://dx.doi.org/10.1016/j.tranon.2020.100945 |
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author | Li, Liren Zhou, Wenhao Li, Qian Li, Pansong Yang, Ling Xia, Xuefeng Yi, Xin Wan, Desen |
author_facet | Li, Liren Zhou, Wenhao Li, Qian Li, Pansong Yang, Ling Xia, Xuefeng Yi, Xin Wan, Desen |
author_sort | Li, Liren |
collection | PubMed |
description | The mutation in postoperative plasma (molecular residues) was an independently prognostic factor in colorectal cancer (CRC). The status of postoperative plasma mutation of microsatellite instability (MSI) CRC has not been systematically examined. In this study, we enrolled 30 MSI and 46 microsatellite stability (MSS) CRCs, and performed next generation sequencing on surgical tissues, postoperative plasma, and plasma during follow-up. Compared with MSS, MSI tumors had dissimilar genomic profiles, higher tumor mutation burden (TMB), and more frameshift mutations. In the postoperative plasma, more MSI CRCs were detected with tumor-derived mutations (77% in MSI vs 33% in MSS, p < 0.001). The numbers of postoperative mutations were proportional to MSI tissues (Spearman r = 0.47, p = 0.023), while not for MSS. More proportion of postoperative plasma samples of MSI CRCs harbored frameshift mutations than MSS (p = 0.007). For the follow-up plasma, 93% (14 out of 15) MSI CRCs harbored tumor-derived mutations; 33% (4/12) MSS were mutation-positive, lower than MSI (p = 0.003). Thus, considering that MSI CRC had extremely distinct mutational characteristics in tumor and postoperative plasma compared with MSS CRC, we propose that the prognostic value of molecular residue identification in postoperative plasma needs to be independently evaluated in MSI and MSS CRCs. |
format | Online Article Text |
id | pubmed-7674603 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Neoplasia Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-76746032020-12-07 Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability Li, Liren Zhou, Wenhao Li, Qian Li, Pansong Yang, Ling Xia, Xuefeng Yi, Xin Wan, Desen Transl Oncol Original article The mutation in postoperative plasma (molecular residues) was an independently prognostic factor in colorectal cancer (CRC). The status of postoperative plasma mutation of microsatellite instability (MSI) CRC has not been systematically examined. In this study, we enrolled 30 MSI and 46 microsatellite stability (MSS) CRCs, and performed next generation sequencing on surgical tissues, postoperative plasma, and plasma during follow-up. Compared with MSS, MSI tumors had dissimilar genomic profiles, higher tumor mutation burden (TMB), and more frameshift mutations. In the postoperative plasma, more MSI CRCs were detected with tumor-derived mutations (77% in MSI vs 33% in MSS, p < 0.001). The numbers of postoperative mutations were proportional to MSI tissues (Spearman r = 0.47, p = 0.023), while not for MSS. More proportion of postoperative plasma samples of MSI CRCs harbored frameshift mutations than MSS (p = 0.007). For the follow-up plasma, 93% (14 out of 15) MSI CRCs harbored tumor-derived mutations; 33% (4/12) MSS were mutation-positive, lower than MSI (p = 0.003). Thus, considering that MSI CRC had extremely distinct mutational characteristics in tumor and postoperative plasma compared with MSS CRC, we propose that the prognostic value of molecular residue identification in postoperative plasma needs to be independently evaluated in MSI and MSS CRCs. Neoplasia Press 2020-11-12 /pmc/articles/PMC7674603/ /pubmed/33190041 http://dx.doi.org/10.1016/j.tranon.2020.100945 Text en © 2020 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original article Li, Liren Zhou, Wenhao Li, Qian Li, Pansong Yang, Ling Xia, Xuefeng Yi, Xin Wan, Desen Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability |
title | Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability |
title_full | Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability |
title_fullStr | Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability |
title_full_unstemmed | Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability |
title_short | Tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability |
title_sort | tumor-derived mutations in postoperative plasma of colorectal cancer with microsatellite instability |
topic | Original article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674603/ https://www.ncbi.nlm.nih.gov/pubmed/33190041 http://dx.doi.org/10.1016/j.tranon.2020.100945 |
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