Recent Progress in the Understanding and Engineering of Coenzyme B(12)-Dependent Glycerol Dehydratase

Coenzyme B(12)-dependent glycerol dehydratase (GDHt) catalyzes the dehydration reaction of glycerol in the presence of adenosylcobalamin to yield 3-hydroxypropanal (3-HPA), which can be converted biologically to versatile platform chemicals such as 1,3-propanediol and 3-hydroxypropionic acid. Owing...

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Autores principales: Nasir, Abdul, Ashok, Somasundar, Shim, Jeung Yeop, Park, Sunghoon, Yoo, Tae Hyeon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674605/
https://www.ncbi.nlm.nih.gov/pubmed/33224925
http://dx.doi.org/10.3389/fbioe.2020.500867
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author Nasir, Abdul
Ashok, Somasundar
Shim, Jeung Yeop
Park, Sunghoon
Yoo, Tae Hyeon
author_facet Nasir, Abdul
Ashok, Somasundar
Shim, Jeung Yeop
Park, Sunghoon
Yoo, Tae Hyeon
author_sort Nasir, Abdul
collection PubMed
description Coenzyme B(12)-dependent glycerol dehydratase (GDHt) catalyzes the dehydration reaction of glycerol in the presence of adenosylcobalamin to yield 3-hydroxypropanal (3-HPA), which can be converted biologically to versatile platform chemicals such as 1,3-propanediol and 3-hydroxypropionic acid. Owing to the increased demand for biofuels, developing biological processes based on glycerol, which is a byproduct of biodiesel production, has attracted considerable attention recently. In this review, we will provide updates on the current understanding of the catalytic mechanism and structure of coenzyme B(12)-dependent GDHt, and then summarize the results of engineering attempts, with perspectives on future directions in its engineering.
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spelling pubmed-76746052020-11-19 Recent Progress in the Understanding and Engineering of Coenzyme B(12)-Dependent Glycerol Dehydratase Nasir, Abdul Ashok, Somasundar Shim, Jeung Yeop Park, Sunghoon Yoo, Tae Hyeon Front Bioeng Biotechnol Bioengineering and Biotechnology Coenzyme B(12)-dependent glycerol dehydratase (GDHt) catalyzes the dehydration reaction of glycerol in the presence of adenosylcobalamin to yield 3-hydroxypropanal (3-HPA), which can be converted biologically to versatile platform chemicals such as 1,3-propanediol and 3-hydroxypropionic acid. Owing to the increased demand for biofuels, developing biological processes based on glycerol, which is a byproduct of biodiesel production, has attracted considerable attention recently. In this review, we will provide updates on the current understanding of the catalytic mechanism and structure of coenzyme B(12)-dependent GDHt, and then summarize the results of engineering attempts, with perspectives on future directions in its engineering. Frontiers Media S.A. 2020-11-05 /pmc/articles/PMC7674605/ /pubmed/33224925 http://dx.doi.org/10.3389/fbioe.2020.500867 Text en Copyright © 2020 Nasir, Ashok, Shim, Park and Yoo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Nasir, Abdul
Ashok, Somasundar
Shim, Jeung Yeop
Park, Sunghoon
Yoo, Tae Hyeon
Recent Progress in the Understanding and Engineering of Coenzyme B(12)-Dependent Glycerol Dehydratase
title Recent Progress in the Understanding and Engineering of Coenzyme B(12)-Dependent Glycerol Dehydratase
title_full Recent Progress in the Understanding and Engineering of Coenzyme B(12)-Dependent Glycerol Dehydratase
title_fullStr Recent Progress in the Understanding and Engineering of Coenzyme B(12)-Dependent Glycerol Dehydratase
title_full_unstemmed Recent Progress in the Understanding and Engineering of Coenzyme B(12)-Dependent Glycerol Dehydratase
title_short Recent Progress in the Understanding and Engineering of Coenzyme B(12)-Dependent Glycerol Dehydratase
title_sort recent progress in the understanding and engineering of coenzyme b(12)-dependent glycerol dehydratase
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674605/
https://www.ncbi.nlm.nih.gov/pubmed/33224925
http://dx.doi.org/10.3389/fbioe.2020.500867
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