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Physiological Mechanisms of Improved Smut Resistance in Sugarcane Through Application of Silicon

Sugarcane smut caused by Sporisorium scitamineum is a severe, global sugarcane disease with severe economic losses and is difficult to prevent. To explore more effective control techniques for smut, the effects and physiological mechanism of silicon (Si) on smut resistance in two smut-susceptible cu...

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Autores principales: Deng, Quanqing, Wu, Jia, Chen, Jianwen, Shen, Wankuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674639/
https://www.ncbi.nlm.nih.gov/pubmed/33224161
http://dx.doi.org/10.3389/fpls.2020.568130
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author Deng, Quanqing
Wu, Jia
Chen, Jianwen
Shen, Wankuan
author_facet Deng, Quanqing
Wu, Jia
Chen, Jianwen
Shen, Wankuan
author_sort Deng, Quanqing
collection PubMed
description Sugarcane smut caused by Sporisorium scitamineum is a severe, global sugarcane disease with severe economic losses and is difficult to prevent. To explore more effective control techniques for smut, the effects and physiological mechanism of silicon (Si) on smut resistance in two smut-susceptible cultivars, ROC22 and Badila, were investigated. The results show that Si application significantly enhances smut resistance in ROC22 and Badila, and the incidence of sugarcane smut decreased by 11.57–22.58% (ROC22) and 27.75–46.67% (Badila). The incidence of smut is negatively correlated with the amount of Si applied and the Si content in sugarcane leaves, stems, and roots (highly significantly negatively correlated with stem Si content). Under S. scitamineum stress, the activities of pathogenesis-related enzymes, chitinase and β-1,3-glucanase, secondary metabolism-related enzymes such as polyphenoloxidase (PPO) and phenylalanine-ammonia-lyase (PAL), and the contents of secondary metabolites, total soluble phenol, and lignin in sugarcane leaves treated with Si were significantly higher than those without Si (CK). The results also demonstrated that the content of malondialdehyde (MDA) and hydrogen peroxide (H(2)O(2)), the superoxide dismutase (SOD) activity of sugarcane leaves treated with Si increased in the seedling and tillering stages, and the peroxidase (POD) activity decreased in the seedling stage, which caused the accumulation of reactive oxygen species (ROS) that in turn triggered defense responses. Moreover, MDA and H(2)O(2) levels decreased, and the activities of SOD and POD increased at the jointing stage, which was beneficial to the removal of excessive ROS. Collectively, these results suggest that Si modulates pathogenesis-related protein activity, secondary metabolism, and active oxygen metabolism of sugarcane that positively regulate resistance to smut. This study is the first to reveal the physiological mechanism of Si in improving smut resistance in sugarcane, and the results provide a theoretical basis for the development of Si fertilizers to control sugarcane smut.
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spelling pubmed-76746392020-11-19 Physiological Mechanisms of Improved Smut Resistance in Sugarcane Through Application of Silicon Deng, Quanqing Wu, Jia Chen, Jianwen Shen, Wankuan Front Plant Sci Plant Science Sugarcane smut caused by Sporisorium scitamineum is a severe, global sugarcane disease with severe economic losses and is difficult to prevent. To explore more effective control techniques for smut, the effects and physiological mechanism of silicon (Si) on smut resistance in two smut-susceptible cultivars, ROC22 and Badila, were investigated. The results show that Si application significantly enhances smut resistance in ROC22 and Badila, and the incidence of sugarcane smut decreased by 11.57–22.58% (ROC22) and 27.75–46.67% (Badila). The incidence of smut is negatively correlated with the amount of Si applied and the Si content in sugarcane leaves, stems, and roots (highly significantly negatively correlated with stem Si content). Under S. scitamineum stress, the activities of pathogenesis-related enzymes, chitinase and β-1,3-glucanase, secondary metabolism-related enzymes such as polyphenoloxidase (PPO) and phenylalanine-ammonia-lyase (PAL), and the contents of secondary metabolites, total soluble phenol, and lignin in sugarcane leaves treated with Si were significantly higher than those without Si (CK). The results also demonstrated that the content of malondialdehyde (MDA) and hydrogen peroxide (H(2)O(2)), the superoxide dismutase (SOD) activity of sugarcane leaves treated with Si increased in the seedling and tillering stages, and the peroxidase (POD) activity decreased in the seedling stage, which caused the accumulation of reactive oxygen species (ROS) that in turn triggered defense responses. Moreover, MDA and H(2)O(2) levels decreased, and the activities of SOD and POD increased at the jointing stage, which was beneficial to the removal of excessive ROS. Collectively, these results suggest that Si modulates pathogenesis-related protein activity, secondary metabolism, and active oxygen metabolism of sugarcane that positively regulate resistance to smut. This study is the first to reveal the physiological mechanism of Si in improving smut resistance in sugarcane, and the results provide a theoretical basis for the development of Si fertilizers to control sugarcane smut. Frontiers Media S.A. 2020-11-05 /pmc/articles/PMC7674639/ /pubmed/33224161 http://dx.doi.org/10.3389/fpls.2020.568130 Text en Copyright © 2020 Deng, Wu, Chen and Shen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Deng, Quanqing
Wu, Jia
Chen, Jianwen
Shen, Wankuan
Physiological Mechanisms of Improved Smut Resistance in Sugarcane Through Application of Silicon
title Physiological Mechanisms of Improved Smut Resistance in Sugarcane Through Application of Silicon
title_full Physiological Mechanisms of Improved Smut Resistance in Sugarcane Through Application of Silicon
title_fullStr Physiological Mechanisms of Improved Smut Resistance in Sugarcane Through Application of Silicon
title_full_unstemmed Physiological Mechanisms of Improved Smut Resistance in Sugarcane Through Application of Silicon
title_short Physiological Mechanisms of Improved Smut Resistance in Sugarcane Through Application of Silicon
title_sort physiological mechanisms of improved smut resistance in sugarcane through application of silicon
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674639/
https://www.ncbi.nlm.nih.gov/pubmed/33224161
http://dx.doi.org/10.3389/fpls.2020.568130
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