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Effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception

OBJECTIVE: To explore the effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception. METHODS: This is a prospective randomized controlled study in which 60 children with primary intussusception admitted to Hebei Children’s Hospita...

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Autores principales: Li, Yang, Jiao, Han-liang, Bai, Yu-kun, Wang, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Professional Medical Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674862/
https://www.ncbi.nlm.nih.gov/pubmed/33235589
http://dx.doi.org/10.12669/pjms.36.7.3105
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author Li, Yang
Jiao, Han-liang
Bai, Yu-kun
Wang, Ping
author_facet Li, Yang
Jiao, Han-liang
Bai, Yu-kun
Wang, Ping
author_sort Li, Yang
collection PubMed
description OBJECTIVE: To explore the effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception. METHODS: This is a prospective randomized controlled study in which 60 children with primary intussusception admitted to Hebei Children’s Hospital from October 2018 to October 2019 were selected for this study. They were randomly divided into two groups. The 30 patients in the experimental group underwent manipulative reduction and air enema reduction, and 30 patients in the control group underwent only air enema reduction. Pain scores and pressure during enema were recorded and analyzed. Fasting blood of children in the experimental group were drawn to test the serum T lymphocyte subsets CD3+, CD4+, CD8+ levels, B lymphocyte subsets CD19+ level, and NK cell subsets CD56+ levels before reduction. Among them, fasting blood of 28 children with successful reduction were drawn again in the morning after reduction, and the indicators of each immune cell subgroup before and after reduction were analyzed. Two children with unsuccessful reduction were no longer tested for these indicators. RESULTS: Twenty-Eight children in the experimental group had successful reduction, and two children with unsuccessful reduction were changed to open surgery (28/30). Twenty five Children in the control group had successful reduction, and five were changed to open surgery (25/30). There was no significant difference in the success rate of reduction between two groups (p>0.05). Close observation for 12~24h after reduction found that none of the children had signs of peritonitis. The pain score and reduction pressure of the observation group were lower than those of the control group, and the difference was statistically significant (p<0.05). The levels of serum CD3+, CD4+, and CD8+ after reduction in the experimental group were significantly higher than before reduction, and the difference was statistically significant (p<0.05). CD19+ level was significantly lower than before reduction, and the difference was statistically significant (p<0.05). There was no significant difference in changes of other indicators. CONCLUSIONS: Manipulative reduction combined with air enema reduction can relieve pain and air injection pressure during enema, reduce reperfusion injury caused by intestinal ischemia, and protect intestinal mucosal immune function, which is a favored treatment.
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spelling pubmed-76748622020-11-23 Effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception Li, Yang Jiao, Han-liang Bai, Yu-kun Wang, Ping Pak J Med Sci Original Article OBJECTIVE: To explore the effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception. METHODS: This is a prospective randomized controlled study in which 60 children with primary intussusception admitted to Hebei Children’s Hospital from October 2018 to October 2019 were selected for this study. They were randomly divided into two groups. The 30 patients in the experimental group underwent manipulative reduction and air enema reduction, and 30 patients in the control group underwent only air enema reduction. Pain scores and pressure during enema were recorded and analyzed. Fasting blood of children in the experimental group were drawn to test the serum T lymphocyte subsets CD3+, CD4+, CD8+ levels, B lymphocyte subsets CD19+ level, and NK cell subsets CD56+ levels before reduction. Among them, fasting blood of 28 children with successful reduction were drawn again in the morning after reduction, and the indicators of each immune cell subgroup before and after reduction were analyzed. Two children with unsuccessful reduction were no longer tested for these indicators. RESULTS: Twenty-Eight children in the experimental group had successful reduction, and two children with unsuccessful reduction were changed to open surgery (28/30). Twenty five Children in the control group had successful reduction, and five were changed to open surgery (25/30). There was no significant difference in the success rate of reduction between two groups (p>0.05). Close observation for 12~24h after reduction found that none of the children had signs of peritonitis. The pain score and reduction pressure of the observation group were lower than those of the control group, and the difference was statistically significant (p<0.05). The levels of serum CD3+, CD4+, and CD8+ after reduction in the experimental group were significantly higher than before reduction, and the difference was statistically significant (p<0.05). CD19+ level was significantly lower than before reduction, and the difference was statistically significant (p<0.05). There was no significant difference in changes of other indicators. CONCLUSIONS: Manipulative reduction combined with air enema reduction can relieve pain and air injection pressure during enema, reduce reperfusion injury caused by intestinal ischemia, and protect intestinal mucosal immune function, which is a favored treatment. Professional Medical Publications 2020 /pmc/articles/PMC7674862/ /pubmed/33235589 http://dx.doi.org/10.12669/pjms.36.7.3105 Text en Copyright: © Pakistan Journal of Medical Sciences http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Li, Yang
Jiao, Han-liang
Bai, Yu-kun
Wang, Ping
Effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception
title Effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception
title_full Effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception
title_fullStr Effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception
title_full_unstemmed Effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception
title_short Effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception
title_sort effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674862/
https://www.ncbi.nlm.nih.gov/pubmed/33235589
http://dx.doi.org/10.12669/pjms.36.7.3105
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