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Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction
OBJECTIVE: To determine genotype frequency of biallelic single nucleotide polymorphisms and its use in detection of informative allele in donor/recipient pairs (sibling pairs) having undergone haematopoietic stem cell transplantation with various haematological disorders using a PCR based method. ME...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Professional Medical Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674877/ https://www.ncbi.nlm.nih.gov/pubmed/33235576 http://dx.doi.org/10.12669/pjms.36.7.2998 |
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author | Nayyar, Ayesha Ahmed, Suhaib |
author_facet | Nayyar, Ayesha Ahmed, Suhaib |
author_sort | Nayyar, Ayesha |
collection | PubMed |
description | OBJECTIVE: To determine genotype frequency of biallelic single nucleotide polymorphisms and its use in detection of informative allele in donor/recipient pairs (sibling pairs) having undergone haematopoietic stem cell transplantation with various haematological disorders using a PCR based method. METHODS: This descriptive study was conducted at GRC Lab Rawalpindi from Jan 2018- Oct 2019.A total of twenty donor/ recipient pairs (sibling pairs) were studied for genotype frequency and informativeness of single nucleotide polymorphisms. Genomic DNA was extracted from the peripheral blood and amplification of single nucleotide polymorphisms was done by PCR based method. The amplified DNA was seen by electrophoresis on 6% polyacrylamide gel. RESULTS: A sharp band of DNA on the polyacrylamide gel indicated a positive reaction. At least two or more informative SNP markers were found in every sibling pair. CONCLUSION: Our results demonstrate that PCR amplification of polyacrylamide gel electrophoresis using single nucleotide polymorphism has allowed the successful screening and detection of informative allele in all the donor/recipient pairs. (Sibling pairs). This PCR based assay using SNPs appears to be a quick, simple, reliable and technically feasible method for a use in a Pakistani setting. |
format | Online Article Text |
id | pubmed-7674877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Professional Medical Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-76748772020-11-23 Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction Nayyar, Ayesha Ahmed, Suhaib Pak J Med Sci Original Article OBJECTIVE: To determine genotype frequency of biallelic single nucleotide polymorphisms and its use in detection of informative allele in donor/recipient pairs (sibling pairs) having undergone haematopoietic stem cell transplantation with various haematological disorders using a PCR based method. METHODS: This descriptive study was conducted at GRC Lab Rawalpindi from Jan 2018- Oct 2019.A total of twenty donor/ recipient pairs (sibling pairs) were studied for genotype frequency and informativeness of single nucleotide polymorphisms. Genomic DNA was extracted from the peripheral blood and amplification of single nucleotide polymorphisms was done by PCR based method. The amplified DNA was seen by electrophoresis on 6% polyacrylamide gel. RESULTS: A sharp band of DNA on the polyacrylamide gel indicated a positive reaction. At least two or more informative SNP markers were found in every sibling pair. CONCLUSION: Our results demonstrate that PCR amplification of polyacrylamide gel electrophoresis using single nucleotide polymorphism has allowed the successful screening and detection of informative allele in all the donor/recipient pairs. (Sibling pairs). This PCR based assay using SNPs appears to be a quick, simple, reliable and technically feasible method for a use in a Pakistani setting. Professional Medical Publications 2020 /pmc/articles/PMC7674877/ /pubmed/33235576 http://dx.doi.org/10.12669/pjms.36.7.2998 Text en Copyright: © Pakistan Journal of Medical Sciences http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nayyar, Ayesha Ahmed, Suhaib Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction |
title | Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction |
title_full | Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction |
title_fullStr | Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction |
title_full_unstemmed | Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction |
title_short | Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction |
title_sort | genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674877/ https://www.ncbi.nlm.nih.gov/pubmed/33235576 http://dx.doi.org/10.12669/pjms.36.7.2998 |
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