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Biostimulative effect of laser on growth of mesenchymal stem/stromal cells in vitro
INTRODUCTION: Human adipose tissue-derived mesenchymal stem/stromal cells (hAT-MSCs) are multipotent stromal cells with a high potential application in tissue engineering and regenerative medicine. Laser irradiation of the place where the cells were implanted can stimulate their proliferation, incre...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675089/ https://www.ncbi.nlm.nih.gov/pubmed/33240019 http://dx.doi.org/10.5114/ada.2020.100487 |
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author | Siedlecka, Natalia Mecinska-Jundzill, Kaja Fierek, Ewelina Fekner, Zuzanna Jundzill, Arkadiusz Rasmus, Marta Kloskowski, Tomasz Szeliski, Kamil Bienkowski, Wieslaw Czajkowski, Rafal Drewa, Tomasz Pokrywczynska, Marta |
author_facet | Siedlecka, Natalia Mecinska-Jundzill, Kaja Fierek, Ewelina Fekner, Zuzanna Jundzill, Arkadiusz Rasmus, Marta Kloskowski, Tomasz Szeliski, Kamil Bienkowski, Wieslaw Czajkowski, Rafal Drewa, Tomasz Pokrywczynska, Marta |
author_sort | Siedlecka, Natalia |
collection | PubMed |
description | INTRODUCTION: Human adipose tissue-derived mesenchymal stem/stromal cells (hAT-MSCs) are multipotent stromal cells with a high potential application in tissue engineering and regenerative medicine. Laser irradiation of the place where the cells were implanted can stimulate their proliferation, increase the secretion of growth factors and thus increase the therapeutic effect. AIM: To evaluate the influence of two lasers: Er:YAG and diode on the growth of hAT-MSCs in vitro. MATERIAL AND METHODS: hAT-MSCs were isolated from human subcutaneous adipose tissue. Immunophenotype of hAT-MSCs was confirmed by flow cytometry. Multipotency of hAT-MSCs was confirmed by differentiation into adipogenic, osteogenic and chondrogenic lineages. hAT-MSCs were irradiated with Er:YAG laser (wavelength 2940 nm, frequency 5, 10 Hz, doses: 0.1–1.2 J/cm(2)) for 2 s and 4 s and diode laser (wavelength 635 nm and doses: 1–8 J/cm(2)) for 5, 10, 20, 30 and 40 s. Cell viability was analysed 24 h after the exposure using MTT assay. RESULTS: Growth stimulation of hAT-MSCs after 5 Hz Er:YAG laser exposure, 0.1 J/cm(2) dose for 4 s and 0.3 J/cm(2) dose for 4 s was shown in comparison with the control group. Significant growth stimulation of hAT-MSCs after diode laser irradiation in doses of 1–4 J/cm(2) was demonstrated compared to the control group. CONCLUSIONS: The presented results indicate that both lasers, Er:YAG and diode can be used to stimulate stem/stromal cell growth in vitro. The biostimulative effect of laser therapy on stromal cells may be used in the future in aesthetic dermatology in combined laser and cell therapy. |
format | Online Article Text |
id | pubmed-7675089 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-76750892020-11-24 Biostimulative effect of laser on growth of mesenchymal stem/stromal cells in vitro Siedlecka, Natalia Mecinska-Jundzill, Kaja Fierek, Ewelina Fekner, Zuzanna Jundzill, Arkadiusz Rasmus, Marta Kloskowski, Tomasz Szeliski, Kamil Bienkowski, Wieslaw Czajkowski, Rafal Drewa, Tomasz Pokrywczynska, Marta Postepy Dermatol Alergol Original Paper INTRODUCTION: Human adipose tissue-derived mesenchymal stem/stromal cells (hAT-MSCs) are multipotent stromal cells with a high potential application in tissue engineering and regenerative medicine. Laser irradiation of the place where the cells were implanted can stimulate their proliferation, increase the secretion of growth factors and thus increase the therapeutic effect. AIM: To evaluate the influence of two lasers: Er:YAG and diode on the growth of hAT-MSCs in vitro. MATERIAL AND METHODS: hAT-MSCs were isolated from human subcutaneous adipose tissue. Immunophenotype of hAT-MSCs was confirmed by flow cytometry. Multipotency of hAT-MSCs was confirmed by differentiation into adipogenic, osteogenic and chondrogenic lineages. hAT-MSCs were irradiated with Er:YAG laser (wavelength 2940 nm, frequency 5, 10 Hz, doses: 0.1–1.2 J/cm(2)) for 2 s and 4 s and diode laser (wavelength 635 nm and doses: 1–8 J/cm(2)) for 5, 10, 20, 30 and 40 s. Cell viability was analysed 24 h after the exposure using MTT assay. RESULTS: Growth stimulation of hAT-MSCs after 5 Hz Er:YAG laser exposure, 0.1 J/cm(2) dose for 4 s and 0.3 J/cm(2) dose for 4 s was shown in comparison with the control group. Significant growth stimulation of hAT-MSCs after diode laser irradiation in doses of 1–4 J/cm(2) was demonstrated compared to the control group. CONCLUSIONS: The presented results indicate that both lasers, Er:YAG and diode can be used to stimulate stem/stromal cell growth in vitro. The biostimulative effect of laser therapy on stromal cells may be used in the future in aesthetic dermatology in combined laser and cell therapy. Termedia Publishing House 2020-11-07 2020-10 /pmc/articles/PMC7675089/ /pubmed/33240019 http://dx.doi.org/10.5114/ada.2020.100487 Text en Copyright © 2020 Termedia http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/) |
spellingShingle | Original Paper Siedlecka, Natalia Mecinska-Jundzill, Kaja Fierek, Ewelina Fekner, Zuzanna Jundzill, Arkadiusz Rasmus, Marta Kloskowski, Tomasz Szeliski, Kamil Bienkowski, Wieslaw Czajkowski, Rafal Drewa, Tomasz Pokrywczynska, Marta Biostimulative effect of laser on growth of mesenchymal stem/stromal cells in vitro |
title | Biostimulative effect of laser on growth of mesenchymal stem/stromal cells in vitro |
title_full | Biostimulative effect of laser on growth of mesenchymal stem/stromal cells in vitro |
title_fullStr | Biostimulative effect of laser on growth of mesenchymal stem/stromal cells in vitro |
title_full_unstemmed | Biostimulative effect of laser on growth of mesenchymal stem/stromal cells in vitro |
title_short | Biostimulative effect of laser on growth of mesenchymal stem/stromal cells in vitro |
title_sort | biostimulative effect of laser on growth of mesenchymal stem/stromal cells in vitro |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675089/ https://www.ncbi.nlm.nih.gov/pubmed/33240019 http://dx.doi.org/10.5114/ada.2020.100487 |
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