Detection of cellular micromotion by advanced signal processing

Cellular micromotion—a tiny movement of cell membranes on the nm-µm scale—has been proposed as a pathway for inter-cellular signal transduction and as a label-free proxy signal to neural activity. Here we harness several recent approaches of signal processing to detect such micromotion in video reco...

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Detalles Bibliográficos
Autores principales: Rinner, Stephan, Trentino, Alberto, Url, Heike, Burger, Florian, von Lautz, Julian, Wolfrum, Bernhard, Reinhard, Friedemann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675976/
https://www.ncbi.nlm.nih.gov/pubmed/33208817
http://dx.doi.org/10.1038/s41598-020-77015-z
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author Rinner, Stephan
Trentino, Alberto
Url, Heike
Burger, Florian
von Lautz, Julian
Wolfrum, Bernhard
Reinhard, Friedemann
author_facet Rinner, Stephan
Trentino, Alberto
Url, Heike
Burger, Florian
von Lautz, Julian
Wolfrum, Bernhard
Reinhard, Friedemann
author_sort Rinner, Stephan
collection PubMed
description Cellular micromotion—a tiny movement of cell membranes on the nm-µm scale—has been proposed as a pathway for inter-cellular signal transduction and as a label-free proxy signal to neural activity. Here we harness several recent approaches of signal processing to detect such micromotion in video recordings of unlabeled cells. Our survey includes spectral filtering of the video signal, matched filtering, as well as 1D and 3D convolutional neural networks acting on pixel-wise time-domain data and a whole recording respectively.
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spelling pubmed-76759762020-11-19 Detection of cellular micromotion by advanced signal processing Rinner, Stephan Trentino, Alberto Url, Heike Burger, Florian von Lautz, Julian Wolfrum, Bernhard Reinhard, Friedemann Sci Rep Article Cellular micromotion—a tiny movement of cell membranes on the nm-µm scale—has been proposed as a pathway for inter-cellular signal transduction and as a label-free proxy signal to neural activity. Here we harness several recent approaches of signal processing to detect such micromotion in video recordings of unlabeled cells. Our survey includes spectral filtering of the video signal, matched filtering, as well as 1D and 3D convolutional neural networks acting on pixel-wise time-domain data and a whole recording respectively. Nature Publishing Group UK 2020-11-18 /pmc/articles/PMC7675976/ /pubmed/33208817 http://dx.doi.org/10.1038/s41598-020-77015-z Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rinner, Stephan
Trentino, Alberto
Url, Heike
Burger, Florian
von Lautz, Julian
Wolfrum, Bernhard
Reinhard, Friedemann
Detection of cellular micromotion by advanced signal processing
title Detection of cellular micromotion by advanced signal processing
title_full Detection of cellular micromotion by advanced signal processing
title_fullStr Detection of cellular micromotion by advanced signal processing
title_full_unstemmed Detection of cellular micromotion by advanced signal processing
title_short Detection of cellular micromotion by advanced signal processing
title_sort detection of cellular micromotion by advanced signal processing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675976/
https://www.ncbi.nlm.nih.gov/pubmed/33208817
http://dx.doi.org/10.1038/s41598-020-77015-z
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