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Long noncoding RNA HULC in acute ischemic stroke: Association with disease risk, severity, and recurrence‐free survival and relation with IL‐6, ICAM1, miR‐9, and miR‐195
BACKGROUND: This study aimed to evaluate the clinical role of long noncoding RNA (lncRNA) HULC in acute ischemic stroke (AIS). METHODS: LncRNA HULC in plasma samples from 215 first episode AIS patients and 215 age/gender‐matched non‐AIS controls was detected by reverse transcriptional‐quantitative p...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676200/ https://www.ncbi.nlm.nih.gov/pubmed/32815572 http://dx.doi.org/10.1002/jcla.23500 |
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author | Chen, Xia Zhang, Xihui Su, Cong Huang, Shaoming |
author_facet | Chen, Xia Zhang, Xihui Su, Cong Huang, Shaoming |
author_sort | Chen, Xia |
collection | PubMed |
description | BACKGROUND: This study aimed to evaluate the clinical role of long noncoding RNA (lncRNA) HULC in acute ischemic stroke (AIS). METHODS: LncRNA HULC in plasma samples from 215 first episode AIS patients and 215 age/gender‐matched non‐AIS controls was detected by reverse transcriptional‐quantitative polymerase chain reaction (RT‐qPCR). Then, in AIS patients, interleukin‐6 and intercellular adhesion molecule 1 (ICAM1), as well as microRNA (miR) target of lncRNA HUCL (miR‐9 and miR‐195), were detected by enzyme‐linked immunosorbent assay and RT‐qPCR, respectively. Disease severity was assessed by National Institution of Health stroke scale (NIHSS) score. AIS recurrence or death was recorded, and recurrence‐free survival (RFS) was calculated. RESULTS: LncRNA HULC was increased in AIS patients compared to non‐AIS controls (P < .001), and receiver operating characteristic curve showed that it was correlated with increased AIS risk (area under curve: 0.876, 95% confidence interval: 0.843‐0.908). Meanwhile, lncRNA HULC was positively correlated with NIHSS score (P < .001, r = .456), interleukin‐6 (P < .001, r = .275) and ICAM1 (P < .001, r = .383), whereas negatively correlated with miR‐9 (P < .001, r = −.438) but not miR‐195 (P = .205, r = −.087) in AIS patients. Additionally, miR‐9 was negatively correlated with NIHSS score (P < .001, r = −.335), interleukin‐6 (P = .001, r = −.231), and ICAM1 (P < .001, r = −.280), while miR‐195 was only negatively associated with NIHSS score (P = .041, r = −.139) in AIS patients. Moreover, lncRNA HULC high expression predicted worse RFS (P = .013) in AIS patients. CONCLUSION: LncRNA HULC is correlated with higher AIS risk, increased disease severity and worse prognosis in AIS patients. Meanwhile, it associates with higher IL‐6, elevated ICAM1, and lower miR‐9 AIS patients. |
format | Online Article Text |
id | pubmed-7676200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76762002020-11-24 Long noncoding RNA HULC in acute ischemic stroke: Association with disease risk, severity, and recurrence‐free survival and relation with IL‐6, ICAM1, miR‐9, and miR‐195 Chen, Xia Zhang, Xihui Su, Cong Huang, Shaoming J Clin Lab Anal Research Articles BACKGROUND: This study aimed to evaluate the clinical role of long noncoding RNA (lncRNA) HULC in acute ischemic stroke (AIS). METHODS: LncRNA HULC in plasma samples from 215 first episode AIS patients and 215 age/gender‐matched non‐AIS controls was detected by reverse transcriptional‐quantitative polymerase chain reaction (RT‐qPCR). Then, in AIS patients, interleukin‐6 and intercellular adhesion molecule 1 (ICAM1), as well as microRNA (miR) target of lncRNA HUCL (miR‐9 and miR‐195), were detected by enzyme‐linked immunosorbent assay and RT‐qPCR, respectively. Disease severity was assessed by National Institution of Health stroke scale (NIHSS) score. AIS recurrence or death was recorded, and recurrence‐free survival (RFS) was calculated. RESULTS: LncRNA HULC was increased in AIS patients compared to non‐AIS controls (P < .001), and receiver operating characteristic curve showed that it was correlated with increased AIS risk (area under curve: 0.876, 95% confidence interval: 0.843‐0.908). Meanwhile, lncRNA HULC was positively correlated with NIHSS score (P < .001, r = .456), interleukin‐6 (P < .001, r = .275) and ICAM1 (P < .001, r = .383), whereas negatively correlated with miR‐9 (P < .001, r = −.438) but not miR‐195 (P = .205, r = −.087) in AIS patients. Additionally, miR‐9 was negatively correlated with NIHSS score (P < .001, r = −.335), interleukin‐6 (P = .001, r = −.231), and ICAM1 (P < .001, r = −.280), while miR‐195 was only negatively associated with NIHSS score (P = .041, r = −.139) in AIS patients. Moreover, lncRNA HULC high expression predicted worse RFS (P = .013) in AIS patients. CONCLUSION: LncRNA HULC is correlated with higher AIS risk, increased disease severity and worse prognosis in AIS patients. Meanwhile, it associates with higher IL‐6, elevated ICAM1, and lower miR‐9 AIS patients. John Wiley and Sons Inc. 2020-08-20 /pmc/articles/PMC7676200/ /pubmed/32815572 http://dx.doi.org/10.1002/jcla.23500 Text en © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Chen, Xia Zhang, Xihui Su, Cong Huang, Shaoming Long noncoding RNA HULC in acute ischemic stroke: Association with disease risk, severity, and recurrence‐free survival and relation with IL‐6, ICAM1, miR‐9, and miR‐195 |
title | Long noncoding RNA HULC in acute ischemic stroke: Association with disease risk, severity, and recurrence‐free survival and relation with IL‐6, ICAM1, miR‐9, and miR‐195 |
title_full | Long noncoding RNA HULC in acute ischemic stroke: Association with disease risk, severity, and recurrence‐free survival and relation with IL‐6, ICAM1, miR‐9, and miR‐195 |
title_fullStr | Long noncoding RNA HULC in acute ischemic stroke: Association with disease risk, severity, and recurrence‐free survival and relation with IL‐6, ICAM1, miR‐9, and miR‐195 |
title_full_unstemmed | Long noncoding RNA HULC in acute ischemic stroke: Association with disease risk, severity, and recurrence‐free survival and relation with IL‐6, ICAM1, miR‐9, and miR‐195 |
title_short | Long noncoding RNA HULC in acute ischemic stroke: Association with disease risk, severity, and recurrence‐free survival and relation with IL‐6, ICAM1, miR‐9, and miR‐195 |
title_sort | long noncoding rna hulc in acute ischemic stroke: association with disease risk, severity, and recurrence‐free survival and relation with il‐6, icam1, mir‐9, and mir‐195 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676200/ https://www.ncbi.nlm.nih.gov/pubmed/32815572 http://dx.doi.org/10.1002/jcla.23500 |
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